Anion-Exchange Displacement Centrifugal Partition Chromatography

Maciuk, Alexandre; Renault, Jean‐Hugues; Margraff, Rodolphe; Trébuchet, Philippe; Zèches-Hanrot, Monique; Nuzillard, Jean‐Marc

doi:10.1021/ac049499w

Cited by 22 publications

(24 citation statements)

References 32 publications

(57 reference statements)

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“…As the concentration of the effluent is driven by the concentration of the displacer, an increase in the sample mass causes a lengthening of the elution time for each component of the mixture, without affecting the transition zones. This observation is clearly supported by computer simulation [8,14] (http://helios.univ-reims.fr/Labos/UPRESA6013/LSD/ JmnSoft/CpcSim/index.html). Therefore, the aim of this work was to set up a pilot-scale isolation of sinalbin from an aqueous white mustard seeds extract, using a 5 L capacity CPC apparatus, and to prove that SIXCPC is a high performance separation technique on an industrial scale.…”

Section: Introductionmentioning

confidence: 56%

“…Strong ion-exchange is a specific displacement chromatography mode that was introduced in SIXCPC to purify ionizable molecules such as hydroxycinnamic acids [8], rosmarinic acid [11], or GSLs [10]. Even though the stationary phase in CPC is a liquid, SIXCPC is quite similar to ion-exchange chromatography on solid resins.…”

Section: The General Methodsmentioning

confidence: 99%

“…Renault and coworkers [8] recently reported an efficient method for the isolation of GSLs from crude plant homogenates that rely upon strong ion-exchange centrifugal partition chromatography (SIXCPC). CPC is a particular kind of liquid countercurrent chromatography in which a biphasic solvent system is used to partition analytes between two liquid phases.…”

Section: Introductionmentioning

confidence: 99%

“…The strong ion-exchange mode we used in this work is a specific displacement chromatographic mode that is used in CPC to isolate ionic analytes [8,10,11]. For this purpose, a lipophilic ion-exchanger is dissolved in the organic stationary phase in order to extract the analytes into this phase through ionic associations during the injection step.…”

Section: Introductionmentioning

confidence: 99%

“…Then, the displacer-containing aqueous mobile phase is pumped through the stationary phase, thus forcing the ionic analytes to be displaced in the aqueous mobile phase and to progress through the column. Previous work has demonstrated that the ionic liquid Aliquat 336m (the exchanger) and an iodide solution (the displacer) were convenient for the purification of GSLs on a laboratory scale [8].…”

Section: Introductionmentioning

confidence: 99%

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Pilot‐scale ion‐exchange centrifugal partition chromatography: Purification of sinalbin from white mustard seeds

Toribio¹,

Pinel²,

Boudesocque³

et al. 2009

J of Separation Science

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Original PaperPilot-scale ion-exchange centrifugal partition chromatography: Purification of sinalbin from white mustard seedsThe purification of p-hydroxybenzylglucosinolate (sinalbin) on a multigram scale from a crude aqueous extract of white mustard seeds (Sinapis alba var. concerta) was successfully achieved by scaling up a strong ion-exchange centrifugal partition chromatography (SIXCPC) laboratory procedure. Thus, the one-step sinalbin purification was performed with 2.35 g of crude extract in l170 min (830 mg/h) up to 70.3 g in l160 min (26.3 g/h) by switching from a 200 mL laboratory scale column to a 5.7 L pilot-scale column. The required biphasic solvent system contained ethyl acetate, nbutanol, and water in 3:2:5 v/v/v proportions, Aliquat 336m (trioctylmethyl ammonium chloride) was added to the organic stationary phase (80 mM) and acted as ionexchanger. Potassium iodide in the aqueous mobile phase (80 mM) was used as sinalbin displacer. The 28.5 mass scale factor arose from the increase in mobile phase flow-rate (from 2 to 50 mL/min), from the higher mass of injected white mustard seed extract (from 12 to 350 g), and from the calculated productivity (from 830 mg to 26.3 g). These results demonstrate that industry scale production of glucosinolates is easily performed by SIXCPC, thus providing pure reference standards for pharmacology studies.

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Section: Introductionmentioning

confidence: 56%

Section: The General Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pilot‐scale ion‐exchange centrifugal partition chromatography: Purification of sinalbin from white mustard seeds

Toribio¹,

Pinel²,

Boudesocque³

et al. 2009

J of Separation Science

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Counter‐Current Chromatography in the Separation of Enantiomers

Pérez

Minguillón

2006

Chiral Separation Techniques

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Countercurrent Chromatography, Scope and Perspectives: Application to Chirotechnology

Minguillón

2011

Chem Eng & Technol

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Countercurrent chromatography (CCC) is a liquid-liquid separation technique particularly adapted to preparative separations. In spite of the broad range of applications possible, CCC is mainly applied to the purification of natural products from complex mixtures, field in which some of the characteristics of CCC make it advantageous over other more common chromatographic modalities. Nevertheless, CCC has much to offer in biotechnological purification and enantioseparation. The general characteristics, advantages and drawbacks of CCC, together with its application in dieverse contexts, in paricular enantioseparations, are discussed. IntroductionThe purity requirements demanded in the production of biopharmaceuticals and fine chemicals for the manufacture of active pharmaceutical ingredients (API) have led to a renewed and widespread interest in preparative chromatography [1,2]. The theoretical understanding of the main factors controlling the process, and therefore the possibility to predict its outcome [3,4], has promoted a change in the industrial mentality from the previous reticence. After the wide realization of the cost effectiveness of this purification approach, chromatography has recently become the preferred method for rapidly accessing products of high added value, such as enantiomers [5,6] and biopharmaceuticals [7,8]. Simulated moving bed (SMB), a continuous liquid-solid technique that permits an improved use of the expensive stationary phase, has played a significant role in bringing chromatography closer to the industrial users. Nevertheless, one of the main disadvantages of SMB is the considerable investment required in equipment.Among the possible alternatives easily adapted to be run in continuous mode, countercurrent chromatography (CCC), a liquid-liquid technique in which the separation of components in a mixture is based on their different partitioning into two immiscible liquids [9][10][11][12], can be considered. Although CCC was described in the 1960s [13] and therefore cannot be contemplated as a new technique, in the recent years the increasing development and adoption of preparative chromatography have had a knock-on effect on CCC. As a consequence, the number of publications and studies dealing with CCC has considerably increased. The particular selectivity, high loading capacity and low solvent demand per amount of substrate processed are the reasons that justify the interest in CCC. CCC versus HPLC: Advantages and DrawbacksThe most characteristic feature of CCC, the absence of a solid support, greatly determines its properties, advantages and limitations. Two immiscible liquids, acting as stationary and mobile phase, respectively, are involved in the chromatographic process. The usual way to maintain one of the liquids stationary while the other is passing through requires the establishment of a centrifugal force in the instrument. The difference in density of the two liquids involved completes the job. Nevertheless, to handle liquids in such a way demands moving parts, rotors, gears...

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Anion-Exchange Displacement Centrifugal Partition Chromatography

Cited by 22 publications

References 32 publications

Pilot‐scale ion‐exchange centrifugal partition chromatography: Purification of sinalbin from white mustard seeds

Pilot‐scale ion‐exchange centrifugal partition chromatography: Purification of sinalbin from white mustard seeds

Counter‐Current Chromatography in the Separation of Enantiomers

Countercurrent Chromatography, Scope and Perspectives: Application to Chirotechnology

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