Counter‐Current Chromatography in the Separation of Enantiomers

Pérez, Eva; Minguillón, Cristina

doi:10.1002/9783527611737.ch11

Cited by 1 publication

(1 citation statement)

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“…Counter-current chromatography (CCC) is a liquid-liquid chromatographic technique, without a solid support in which the stationary and the mobile phases are constituted by two immiscible liquids or solutions [4]. Therefore, it is an excellent alternative since the column requires no solid support and can be used to separate a variety of enantiomers simply by adding a suitable chiral selector to the stationary liquid phase.…”

Section: Introductionmentioning

confidence: 99%

Separation of α-cyclohexylmandelic acid enantiomers using biphasic chiral recognition high-speed counter-current chromatography

Tong¹,

Yan²,

Guan³

et al. 2010

Journal of Chromatography A

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This work concentrates on a novel chiral separation technology named biphasic recognition applied to resolution of α-cyclohexylmandelic acid enantiomers by high-speed counter-current chromatography (HSCCC). The biphasic chiral recognition HSCCC was performed by adding lipophilic (−)-2-ethylhexyl tartrate in the organic stationary phase and hydrophilic hydroxypropyl-β-cyclodextrin in the aqueous mobile phase, which preferentially recognized the (−)-enantiomer and (+)-enantiomer, respectively. The two-phase solvent system composed of n-hexane-methyl tert-butyl ether-water (9:1:10, v/v/v) with the above chiral selectors was selected according to the partition coefficient and separation factor of the target enantiomers. Various parameters involved in the chiral separation were investigated, namely the types of the chiral selector (CS); the concentration of each chiral selector; pH of the mobile phase; and the separation temperature. The mechanism involved in this biphasic recognition chiral separation by HSCCC was discussed. Langmuirian isotherm was employed to estimate the loading limits for each chiral selector. The overall experimental results show that the HSCCC separation of enantiomer based on biphasic recognition is much more efficient than the traditional monophasic recognition chiral separation, since it utilizes the cooperation of both lipophilic and hydrophilic chiral selectors.

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Section: Introductionmentioning

confidence: 99%