This research aims to explore the impact of the joint application of 2D animation and augmented reality technology on the learning motivation and achievement of third-year college students learning traditional culture courses. AR is a technology that augments a live view of reality with computer-generated sound, images, or video. It allows users to interact with virtual objects in a real physical environment. Therefore, the researchers developed a learning system called “OperaAR-E” for the “Chaozhou Opera Learning” unit. This research adopts a quasi-experimental design with a pre-test and post-test control group, and uses ARCS questionnaires, achievement tests, observation and interview methods for data collection and research. The independent variable of this research is the “OperaAR-E” learning system, and the dependent variables are learning motivation and learning achievement. The researchers hope to use this learning system to inspire young people to learn traditional culture and gain an in-depth understanding of Chaozhou opera, a traditional Chinese opera form.