Animal Transgenesis and Cloning

Houdebine, Louis

doi:10.1002/0470867280

Cited by 28 publications

(30 citation statements)

References 250 publications

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“…Bacterial, yeast, and higher eukaryotic cells harboring a foreign DNA fragment are known as recombinant or transformed cells (Houdebine, 2003). Gene constructs may stay episomal or integrate into the genome of somatic and/or germ line cells at one or more sites.…”

Section: Definitionsmentioning

confidence: 99%

“…Cloning is the reproduction of a cell or a whole organism without any modification of its genotype (Houdebine, 2003). The ability to duplicate an organism requires totipotency, which is defined as a cell characteristic in which the potential for forming all the cell types in the adult organism is retained (www.sivb.org/edu_terminology.asp).…”

Section: Cloning Via Nuclear Transfer (Nt)mentioning

confidence: 99%

“…(Piedrahita et al, 1999), which is 100 times less frequent than random integration (Houdebine, 2003). Transfected cells are selected with a selection marker and can be used as donor cells accordingly (Gordon, 2003).…”

Section: Cloning Via Nuclear Transfer (Nt)mentioning

confidence: 99%

“…Rodent models as commonly used organisms for biomedical research provide invaluable data for understanding human diseases (Houdebine, 2003), but they have certain limitations due to their small size, short gestation time, different metabolism and limited life span. Transgenic large animal models provide a highly useful tool for biomedical research, particularly in regard to xenotransplantation, chronic diseases, genetic defects or questions concerning the species-specific biological mechanisms, such as embryo-maternal communication .…”

Section: Disease Modelsmentioning

confidence: 99%

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Evaluation of laser-assisted lentiviral transgenesis in bovine

et al. 2006

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Section: Definitionsmentioning

confidence: 99%

Section: Cloning Via Nuclear Transfer (Nt)mentioning

confidence: 99%

Section: Cloning Via Nuclear Transfer (Nt)mentioning

confidence: 99%

Section: Disease Modelsmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of laser-assisted lentiviral transgenesis in bovine

et al. 2006

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“…This paper is a brief survey of animal transgenesis. A more detailed description of the techniques and applications of transgenesis has been reported in a book [1].…”

Section: Introductionmentioning

confidence: 99%

Relations between animal transgenesis and reproduction

Houdebiné¹

2005

Reprod. Nutr. Dev.

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-Transgenesis has become an essential tool for the study of gene expression mechanisms and functions. Transgenesis is also more and more used for biotechnological applications such as the study of human diseases, the adaptation of pig organs to humans, the production of pharmaceutical proteins in milk and likely in the future for the improvement of animal production. The use of transgenesis relies on the efficiency of gene transfer. New tools have been recently designed to improve gene transfer. The methods of gene transfer are highly dependent on the techniques of animal reproduction. Conversely, the need to improve transgenesis urges researchers to study some of the key steps in reproduction and to find new techniques for gene transfer. This paper summarises the recent data and the perspectives offered by animal transgenesis. transgenesis / animal production / biotechnological applications

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Transgenic Animals

Houdebine¹

2014

Encyclopedia of Life Sciences

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Transgenesis implies that a foreign deoxyribonucleic acid (DNA) fragment is introduced into the genome of a multicellular organism and transmitted to progeny. Transgenesis, therefore, differs from gene transfer into cultured cells (transfection) or into the somatic cells of a patient (gene therapy). The foreign DNA can integrate randomly in host genome leading to gene addition or in a targeted manner making it possible precise endogenous gene inactivation or replacement. Transgenesis allows transferring genes from any source in a single generation. Transgenesis is, therefore, complementary to spontaneous mutations, which take place at each reproduction cycle or which are experimentally induced by mutagenic compounds or irradiation. These conventional approaches must be followed by appropriate selection of animals. Transgenesis has become an essential tool to study gene function especially in the medical and pharmaceutical fields. Improvements of breeding and food are also in course. Key Concepts: Transgenesis is more precise and more diverse than conventional selection. Transgenesis and conventional selection are complementary. Transgenesis is an essential tool to study gene action and control. Cloning is presently intensively used to generate transgenic farm animals. Stem cells are currently used for random and targeted gene integration. Transposons and lentiviral vectors are highly efficient for random gene integration. Targeted gene integration using double‐strand genomic DNA break provides researchers with exceptionally efficient new tools. Random gene integration is still intensively used despite its technical limits. Conventional targeted gene transfer is limited by its low efficiency. SiRNAs are efficient tools to inhibit gene expression at the mRNA level. The available tools to generate transgenic animals offer the possibility to develop more relevant biological models and to prepare safer food. Targeted gene transfer should greatly improve transgene expression. Animal behaviourists must participate in conservation planning to protect the future of biodiversity.

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Animal Transgenesis and Cloning

Cited by 28 publications

References 250 publications

Evaluation of laser-assisted lentiviral transgenesis in bovine

Evaluation of laser-assisted lentiviral transgenesis in bovine

Relations between animal transgenesis and reproduction

Transgenic Animals

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