Animal Models of Alcoholic Liver Disease: Pathogenesis and Clinical Relevance

Gao, Bin; Xu, Ming; Bertola, Adeline; Wang, Hua; Zhou, Zhou; Liangpunsakul, Suthat

doi:10.3727/105221617x695519

Cited by 96 publications

(93 citation statements)

References 106 publications

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“…Elevated WBC count was also observed in the animals subjected to excessive alcohol (Gao, et al, 2017). Likewise, we also found ethanol treatment to induce significant elevation in WBC, whereas Clovinol supplementation was found to restore it back to the normal conditions.…”

Section: Discussionsupporting

confidence: 55%

Hepato-protective Effect of Clove Bud Polyphenols (Syzygium aromaticum L.) (Clovinol®) by Modulating Alcohol Induced Oxidative Stress and Inflammation

Jose¹,

Ratheesh²,

Asha³

et al. 2017

JFR

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The pathogenesis of alcoholic liver diseases progresses from fatty liver to hepatic inflammation and necrosis to fibrosis. Clove buds (Syzygium aromaticum L.) are one of the richest sources of dietary polyphenols with many traditional medicinal uses. Hence, the present work attempted to evaluate the hepatoprotective activity of a standardized polyphenol-rich extract of clove buds (Clovinol). The experiment was conducted on Wistar rats designated into three groups. First group was vehicle control and hepatotoxicity was induced to the second group by the administration of ethanol at the fixed dose of 12.5 g/kg body wt., orally for 30 days. Clovinol (100 mg/kg body wt.) was co-administered with ethanol in the third group. The liver toxicity was assessed by the estimation of liver marker enzymes in addition to which, assays of antioxidant enzymes, inflammatory markers, and liver histopathology studies were also conducted. Ethanol treatment significantly increased (p<0.05) liver function markers (SGOT and SGPT) and reduced (p<0.05) the antioxidant enzymes (SOD, CAT, and GPx) and GSH. It also increased the WBC count, inflammatory markers (nitrite, CRP, COX-2, IL-6 and TNF-α) and lipid peroxidation, significantly (p<0.05). Supplementation of Clovinol showed significant (p<0.05) reversal of all these biochemical and molecular variables indicating the efficacy of Clovinol in the downregulation of alcohol-induced oxidative stress and inflammatory changes, bringing about a significant reduction in the associated liver pathology. To summarize, we found that Clovinol could be a potential functional ingredient for liver health.

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Section: Discussionsupporting

confidence: 55%

Hepato-protective Effect of Clove Bud Polyphenols (Syzygium aromaticum L.) (Clovinol®) by Modulating Alcohol Induced Oxidative Stress and Inflammation

Jose¹,

Ratheesh²,

Asha³

et al. 2017

JFR

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“…It has been reported that female mice are more sensitive to developing AFLD. (13,20) Chronic plus binge alcohol feeding significantly suppressed hepatic Cyp7a1 mRNA expression in female mice but not in male mice compared to respective pair-fed control mice ( Fig. 1, left panel).…”

Section: Deficiency Of Cyp7a1 Aggravated Alcohol-induced Liver Injurymentioning

confidence: 94%

Deficiency of cholesterol 7α‐hydroxylase in bile acid synthesis exacerbates alcohol‐induced liver injury in mice

Donepudi

Ferrell

Boehme

et al. 2017

Hepatology Communications

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Alcoholic fatty liver disease (AFLD) is a major risk factor for cirrhosis‐associated liver diseases. Studies demonstrate that alcohol increases serum bile acids in humans and rodents. AFLD has been linked to cholestasis, although the physiologic relevance of increased bile acids in AFLD and the underlying mechanism of increasing the bile acid pool by alcohol feeding are still unclear. In this study, we used mouse models either deficient of or overexpressing cholesterol 7α‐hydroxylase (Cyp7a1), the rate‐limiting and key regulatory enzyme in bile acid synthesis, to study the effect of alcohol drinking in liver metabolism and inflammation. Mice were challenged with chronic ethanol feeding (10 days) plus a binge dose of alcohol by oral gavage (5 g/kg body weight). Alcohol feeding reduced bile acid synthesis gene expression but increased the bile acid pool size, hepatic triglycerides and cholesterol, and inflammation and injury in wild‐type mice and aggravated liver inflammation and injury in Cyp7a1‐deficient mice. Interestingly, alcohol‐induced hepatic inflammation and injury were ameliorated in Cyp7a1 transgenic mice. Conclusion: Alcohol feeding alters hepatic bile acid and cholesterol metabolism to cause liver inflammation and injury, while maintenance of bile acid and cholesterol homeostasis protect against alcohol‐induced hepatic inflammation and injury. Our findings indicate that CYP7A1 plays a key role in protection against alcohol‐induced steatohepatitis. (Hepatology Communications 2018;2:99–112)

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“…Oxidative stress is one of the important mechanisms leading to liver damage (Ana, Alma, Vázquez, Natalia, & Javier, 2016;Gao et al, 2017). Many investigations strongly suggest that liver damage produced by alcohol is mediated through oxidative stress (Albano, 2008;Ambade & Mandrekar, 2012).…”

Section: Effect Of Lbp and Znso 4 On Serum Transaminase Levels In Lmentioning

confidence: 99%

Study on the synergistic protective effect of Lycium barbarum L. polysaccharides and zinc sulfate on chronic alcoholic liver injury in rats

Yan¹,

Lu³

et al. 2019

Food Science & Nutrition

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Both Lycium barbarum L. polysaccharides (LBP) and zinc have protective effects on liver injuries. In this paper, LBP and ZnSO4 were combined to study the effects on the prevention of alcoholic liver injury. The rats were divided into six groups, the normal group, alcohol group, zinc sulfate group, LBP group, low‐dose group of ZnSO4, and high‐dose group of ZnSO4 and LBP, used to explore the impact of LBP and ZnSO4 complex on liver lipid metabolism of alcohol, alcohol‐metabolizing enzymes, oxidative damage, and inflammation of the liver. The experimental model was established by gavage treatment, observation, and determination of indexes of rats. The results showed that the combination of LBP and ZnSO4 could significantly decrease the levels of triglyceride (TG), total cholesterol (TC), tumor necrosis factor‐α(TNF‐ɑ), malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and the activity of enzyme subtype 2E1 (CYP2E1). It also significantly increased the activities of total superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH‐Px), glutathione peptide (GSH), and alcohol dehydrogenase, effectively improved the liver tissue lesion. What is more, the combination of LBP and ZnSO4 had a synergistic effect on the remission of alcoholic fatty liver, and alleviated chronic alcoholic liver injury by promoting lipid metabolism, inhibiting oxidative stress, controlling inflammatory responses, and regulating the expression and activity of alcohol‐metabolizing enzymes in rats.

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Animal Models of Alcoholic Liver Disease: Pathogenesis and Clinical Relevance

Cited by 96 publications

References 106 publications

Hepato-protective Effect of Clove Bud Polyphenols (Syzygium aromaticum L.) (Clovinol®) by Modulating Alcohol Induced Oxidative Stress and Inflammation

Hepato-protective Effect of Clove Bud Polyphenols (Syzygium aromaticum L.) (Clovinol®) by Modulating Alcohol Induced Oxidative Stress and Inflammation

Deficiency of cholesterol 7α‐hydroxylase in bile acid synthesis exacerbates alcohol‐induced liver injury in mice

Study on the synergistic protective effect of Lycium barbarum L. polysaccharides and zinc sulfate on chronic alcoholic liver injury in rats

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