Animal Models for Dermatomycotic Infections

Cutsem, J. Van

doi:10.1007/978-1-4612-3624-5_1

Cited by 24 publications

(19 citation statements)

References 63 publications

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“…erinacei (hedgehog), Trichophyton verrucosum (cattle, horse), T. rubrum (anthropophilic), Trichophyton tonsurans (anthropophilic), Trichophyton asteroids , Trichophyton violaceum (anthropophilic), Trichophyton concentricum (anthropophilic), M. canis , Microsporum gypseum , Microsporum pesicolor , Candida albicans , and Epidermophyton floccosum . In general, zoophilic dermatophytes are more pathogenic to laboratory animals than anthropophilic strains [63, 64]. Thus, T. mentagrophytes and M. canis were specially used for the study of susceptibility to infection [2, 61, 63].…”

Section: Animal Model Of Tinea Corporismentioning

confidence: 99%

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Animal Model of Dermatophytosis

Shimamura

Kubota

Shibuya

2012

Journal of Biomedicine and Biotechnology

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Dermatophytosis is superficial fungal infection caused by dermatophytes that invade the keratinized tissue of humans and animals. Lesions from dermatophytosis exhibit an inflammatory reaction induced to eliminate the invading fungi by using the host's normal immune function. Many scientists have attempted to establish an experimental animal model to elucidate the pathogenesis of human dermatophytosis and evaluate drug efficacy. However, current animal models have several issues. In the present paper, we surveyed reports about the methodology of the dermatophytosis animal model for tinea corporis, tinea pedis, and tinea unguium and discussed future prospects.

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Section: Animal Model Of Tinea Corporismentioning

confidence: 99%

“…In general, zoophilic dermatophytes are more pathogenic to laboratory animals than anthropophilic strains [63, 64]. Thus, T. mentagrophytes and M. canis were specially used for the study of susceptibility to infection [2, 61, 63]. …”

Section: Animal Model Of Tinea Corporismentioning

confidence: 99%

Animal Model of Dermatophytosis

Shimamura

Kubota

Shibuya

2012

Journal of Biomedicine and Biotechnology

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“…Fourteen specifically pathogen-free, three month-old female guinea pigs of the Hartley strain (B & K Universal, Hull, UK) were cutaneously infected with M. canis strain IHEM 15221 (BCCM TM ; IHEM, Brussels, Belgium), essentially as described by Van Cutsem [15]. Briefly, inoculum consisting of mycelia and conidia collected from a 13-day-old slope culture and suspended in a honey-water (2:1 v:v) mixture was applied on a 15-cm 2 dorsal skin area previously clipped and scarified under general anaesthesia.…”

Section: Animals and Experimental Infectionmentioning

confidence: 99%

Humoral and cellular immune response to aMicrosporumcanisrecombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs

Brouta

Descamps²,

Vermout³

et al. 2003

Med Mycol

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In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis.

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“…Specific pathogen free (SPF), 3-month-old female guinea pigs of the Hartley strain were cutaneously infected with M. canis strain IHEM 10478, essentially as previously described [18]. Briefly, the aerial sporulated mycelium of 13-day-old slope cultures on Sabouraud glucose agar was suspended in a mixture of sterile saline and honey (2:1 v/v).…”

Section: Infection Of Guinea Pigsmentioning

confidence: 99%

Humoral and cellular immune response to a crude exo-antigen and purified keratinase ofMicrosporum canisin experimentally infected guinea pigs

Mignon¹,

Leclipteux²,

Focant³

et al. 1999

Med Mycol

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In order to understand better the host-parasite relationship and to compare with previous observations in Microsporum canis naturally infected cats, the humoral and cellular immune responses to both a crude exo-antigen and a 31·5 kDa purified keratinase were evaluated in 12 M. canis experimentally infected guinea pigs. Humoral and cellular responses were assessed by ELISA from days 0 to 56 postinfection (PI) and by measurement of delayed-type hypersensitivity (DTH) responses on days 14 and 57 PI, respectively. Additionally, immunohistochemical staining was performed and demonstrated that the keratinase was produced in infected guinea pig skin, as previously reported in cats. Despite a marked interindividual variation, all the guinea pigs produced specific IgG to the crude exo-antigen from day 21 PI onwards, but no anti-keratinase IgG was detected. Strongly positive DTH responses to the exo-antigen were observed on both dates, whereas the keratinase elicited no and weak DTH on days 14 and 57 PI, respectively. These results are in agreement with those previously described for naturally infected cats, and indicate that the 31·5 kDa keratinase is not a major antigen in M. canis infection.

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Animal Models for Dermatomycotic Infections

Cited by 24 publications

References 63 publications

Animal Model of Dermatophytosis

Animal Model of Dermatophytosis

Humoral and cellular immune response to aMicrosporumcanisrecombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs

Humoral and cellular immune response to a crude exo-antigen and purified keratinase ofMicrosporum canisin experimentally infected guinea pigs

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