Animal Adhesion Models: Design, Variables, and Relevance

Wiseman, David M.

doi:10.1007/978-1-4612-1194-5_38

Cited by 11 publications

(42 citation statements)

References 135 publications

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“…The impact of the specific animal model of peritoneal injury on experimental outcomes and their significance in humans has been well recognized 15. Each animal model has pros and cons, although these are not always very well defined in the literature.…”

Section: Discussionmentioning

confidence: 99%

Biodegradable polymeric microspheres and nanospheres for drug delivery in the peritoneum

Kohane

Tse

Yeo

et al. 2006

J Biomedical Materials Res

196

164

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Drug delivery to the peritoneum is hampered by rapid clearance, and could be improved by application of controlled release technology. We investigated the suitability for peritoneal use of micro- and nanoparticles of poly(lactic-co-glycolic) acid (PLGA), a biodegradable polymer with generally excellent biocompatibility commonly used for controlled drug release. We injected 90 kDa PLGA microparticles, 5-250 microm in diameter, into the murine peritoneum, in dosages of 10-100 mg (n=3-5 per group). We found a high incidence of polymeric residue and adhesions 2 weeks after injection (e.g., 50 mg of 5-microm microparticles caused adhesions in 83% of animals). Histology revealed chronic inflammation, with foreign body giant cells prominent with particles>5 microm in diameter. Five micrometer microspheres made from 54, 57, and 10 kDa PLGA (gamma irradiated) caused fewer adhesions (16.7%) with a similar incidence of residue. Nanoparticles (265 nm) of 90 kDa PLGA also caused much fewer adhesions (6.3% of animals), possibly because they were cleared from the peritoneum within 2 days, and sequestered in the spleen and liver, where foamy macrophages were noted. The effect of sterilization technique on the incidence of adhesion formation is also studied.

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Section: Discussionmentioning

confidence: 99%

Biodegradable polymeric microspheres and nanospheres for drug delivery in the peritoneum

Kohane

Tse

Yeo

et al. 2006

J Biomedical Materials Res

196

164

View full text Add to dashboard Cite

show abstract

“…Several types of animal models have been used, small (mice, rat and rabbit) and large (sheep, pig, monkey and horse) (12). Models do stimulate adhesionformation in different ways, including colon and side wall abrasion, crushing, desiccation, incision, excision, electrocautery, laser injury, thermal injury, chemical injury, radiation injury, and foreign body-tissue irritation (12,13). However, the usefulness of those models is hampered by the variability of adhesion formation in the positive control animals.…”

Section: Discussionmentioning

confidence: 99%

A Novel Rat Model to Test Intra-Abdominal Anti-adhesive Therapy

et al. 2020

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Background: Adhesion formation after abdominal surgery is considered almost inevitable and a major cause of morbidity. Novel treatments have been proposed, however there is a lack of suitable small animal models for pre-clinical evaluation, mainly due to inconsistency in adhesion formation in positive control animals. Here, we propose a new rat model of abdominal adhesions using Kaolin as the adhesion-inducing agent at an optimized dosage for testing newer agents in respect to their anti-adhesive property.Materials and Methods: Twenty-five adult (8-10 week old) male Wistar albino rats underwent midline laparotomy and caecal abrasion and were randomized to receive topical applications of normal saline or different concentrations and volumes of a Kaolin-based formulation. At day 14 rats were humanely killed, and adhesions graded macroscopically by an investigator blinded to the treatment groups, using pre-determined adhesion scores and microscopically using histopathology.Results: Kaolin at 0.005 g/mL caused consistent adhesions without compromising rat viability. At higher doses significant morbidity and mortality was observed in the animals treated.Conclusions: Kaolin induced adhesion in a rat abdominal surgery model is reliable and can be safely used to test the efficacy of novel anti-adhesive formulations to prevent intra-abdominal adhesions.

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“…It should be emphasized that the double injury rabbit model used in this work is significantly more challenging than models used in other studies to evaluate compounds that show good efficacy in animal studies but relatively poorer performance in the clinic [59]. In this light, the observed ~70% reduction in adhesion area is of potential clinical significance for adhesion prevention.…”

Section: Discussionmentioning

confidence: 99%

Prevention of peritoneal adhesions using polymeric rheological blends

et al. 2014

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The effectiveness of rheological blends of high molecular weight hyaluronic acid (HA) and low molecular weight hydroxypropyl methylcellulose (HPMC) in the prevention of peritoneal adhesions post-surgery is demonstrated. The physical mixture of the two carbohydrates increased the dwell time in the peritoneum while significantly improving the injectability of the polymer compared to hyaluronic acid alone. HA-HPMC treatment decreased the total adhesion area by ~70% relative to a saline control or no treatment in a repeated cecal injury model in the rabbit. No significant cytotoxicity and minimal inflammation was associated with the blend, and no chemical or physical processing was required prior to their use beyond simple mixing.

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Animal Adhesion Models: Design, Variables, and Relevance

Cited by 11 publications

References 135 publications

Biodegradable polymeric microspheres and nanospheres for drug delivery in the peritoneum

Biodegradable polymeric microspheres and nanospheres for drug delivery in the peritoneum

A Novel Rat Model to Test Intra-Abdominal Anti-adhesive Therapy

Prevention of peritoneal adhesions using polymeric rheological blends

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