Angiotensin II type 2 receptor is expressed in human sperm cells and is involved in sperm motility

Citores, Marta Gianzo; Muñoa-Hoyos, Iraia; Urizar-Arenaza, Itziar; Larreategui, Zaloa; Quintana, Fernando; Garrido, Nicolás; Subirán, Nerea; Irazusta, Jon

doi:10.1016/j.fertnstert.2015.11.004

Cited by 37 publications

(39 citation statements)

References 48 publications

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“…Apart from the significant correlation with motility, the present study revealed no significant correlation with sperm concentration or with morphology. Gianzo et al, (), on the other hand, detected a significant positive correlation between AT2R expression and sperm concentration but not with morphology. This difference as regards sperm concentration could be attributed to the heterogeneous nature of their study population as regards fertility status.…”

Section: Discussionsupporting

confidence: 92%

“…Nevertheless, its action was believed to be independent of calcium‐mediated signals (Kohn, Muller, et al, ; Wennemuth et al, ). It was also detected on the equatorial and post‐acrosomal regions of the sperm head in humans, which suggests its involvement in sperm functions essential for reproduction (Gianzo et al, ). In the current study, AT2R was located not only on the sperm head including the acrosomal cap, but also on the midpiece and the tail, though the level of expression differed in the studied groups.…”

Section: Discussionmentioning

confidence: 98%

“…In humans, AT2R expression level was found to be positively correlated with progressive motility grade and negatively correlated with the per cent of immotile sperm. In addition, the per cent of AT2R positive spermatozoon was found to be significantly higher in normozoospermic compared with azoospermic males (Gianzo et al, ). In accordance with those findings, significantly lower AT2R expression scores were detected in the present study in the infertile varicocele patients compared with fertile patients and normal controls.…”

Section: Discussionmentioning

confidence: 99%

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Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status

et al. 2020

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Several theories were proposed to explain the pathophysiology of varicocele‐related infertility seen in some patients. Our aim was to study the levels of angiotensin II in semen and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients in relation to their fertility status and to evaluate the influence of varicocelectomy on their levels in infertile varicocele patients. Thirty fertile and 30 infertile varicocele patients and 30 healthy controls were subjected to measurement of reproductive hormones, semen analysis, measurement of seminal angiotensin II and evaluation of angiotensin II type 2 receptor expression on spermatozoa. Infertile varicocele patients underwent varicocelectomy and were re‐evaluated for the same parameters after the operation. Sperm concentration, morphology, progressive motility, seminal angiotensin II and angiotensin II type 2 receptor expression were significantly lower in infertile varicocele patients compared with the other groups. Post‐operative values showed significant increase in the studied parameters compared with the pre‐operative values but not to other two groups. A significant positive correlation between angiotensin II type 2 receptor expression and progressive motility was detected in all studied groups. In conclusion, dysregulation of angiotensin II and angiotensin II type 2 receptor in varicocele patients may be involved in varicocele‐related infertility.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status

et al. 2020

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“…ATR1 activation increases intracellular concentrations of cyclic adenosine monophosphate (cAMP) and calcium, two key secondary messengers of sperm capacitation (Vedantam, Rani, Garg, & Suresh, 2014). In human sperm, ATR2 controls sperm motility (Gianzo et al, 2016). Whether or not tACE participates in this angiotensin-mediated regulation of sperm function is not clear.…”

Section: Discussionmentioning

confidence: 99%

Testis-specific isoform of angiotensin-converting enzyme (tACE) is involved in the regulation of bovine sperm capacitation

2017

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We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine testes and sperm; and (iii) determine the role of tACE in capacitation. A 110-kDa protein, consistent with the mass of tACE, was detected in sperm extract by our anti-tACE immunoserum. This immunotarget localized at the acrosomal region and principal piece, but was only expressed in testis of mature bulls. When bull sperm were incubated in Sp-TALP (0 and 4 hr) plus 10 µg/ml heparin (capacitation group) or 10 µg/ml heparin + 10 µM captopril (an ACE inhibitor) for 4 hr, the number of acrosome-reacted (40.1 vs. 24.0%, respectively) and hyperactivated (15.0 vs. 9.7%) sperm increased, and tyrosine phosphoprotein content were higher (p < 0.05) for sperm in heparin alone. tACE activity was also higher (0.04 U/ml; p < 0.01) in incubation medium of sperm exposed to heparin compared to 0- and 4-hr incubation controls or heparin + captopril conditions (0, 0.005, and 0.009 U/ml, respectively). Furthermore, capacitation-associated shedding of a portion of tACE into the medium decreased sperm content of the 110-kDa tACE, but concurrently increased the abundance of a 60-kDa tACE variant. Thus, a portion of the extracellular region of tACE (containing its catalytic site) is released from bovine sperm during capacitation, and tACE activity may be required for sperm capacitation.

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“…At the same time, intensity was positively correlated with sperm motility. Angiotensin II type 2 receptor has been investigated using flow cytometry in human spermatozoa (Gianzo et al., ). The expression of this protein was reduced in asthenozoospermic patients.…”

Section: Single‐cell Analysis In Sperm Proteomics: Flow Cytometrymentioning

confidence: 99%

Flow cytometry in Spermatology: A bright future ahead

Ortega-Ferrusola

Gil

Rodriguez‐Martinez

et al. 2017

Reprod Domestic Animals

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Techniques such as mass spectrometry have led to unprecedented knowledge of the proteins that are present in the spermatozoa of humans and other mammals. However, in spite of their high-throughput and fractioning techniques, most of the techniques in use only offer average values for the entire sperm population. Yet, ejaculate is very heterogeneous, and average values may mask relevant biological information.The application of flow cytometry may overcome this disadvantage, allowing proteomic analysis at the single-cell level. Moreover, recent advances in cytometry, allowing multiple analyses within a single cell combined with powerful statistical tools, as an expanding subfield in spermatology, are described. The increased use of advanced flow cytometers in andrology laboratories will allow the rapid development of multiparametric, multicolour flow cytometry in andrology that will expand the clinical applications and research possibilities of flow cytometry-based proteomic approaches, especially in the subfields of clinical andrology and sperm biotechnology.

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Angiotensin II type 2 receptor is expressed in human sperm cells and is involved in sperm motility

Cited by 37 publications

References 48 publications

Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status

Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status

Testis-specific isoform of angiotensin-converting enzyme (tACE) is involved in the regulation of bovine sperm capacitation

Flow cytometry in Spermatology: A bright future ahead

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