Angiotensin II regulates phosphoinositide 3 kinase/Akt cascade via a negative crosstalk between AT1 and AT2 receptors in skin fibroblasts of human hypertrophic scars

Characterization of AT1 and AT2 receptor expression profiles in human skin during fetal life

“…This might provide additional evidence that AT 2 receptor plays a role via mediating differentiation process during skin development since the suprabasal cells display morphological signs of differentiation. This concept is further supported by the finding that AT 2 receptor is found in processes involving growth and differentiation such as in wound healing and scarring after skin injury [8,9]. Taken together, we described that the AT 1 and AT 2 receptors are developmentally regulated in fetal skin, suggesting the possible diverse actions that Ang II might play in development and maturation of skin.…”

“…The primary antibodies used in this study were anti-AT 1 and anti-AT 2 receptor antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Total RNA was extracted from frozen fetal skin samples with TRIzol Reagent (Gibco-BRL) and quantitative real-time polymerase chain reaction (PCR) was performed as described previously [8].…”

Additive Effect of TAK-491, a New Angiotensin Receptor Blocker, and Pioglitazone, in Reducing Myocardial Infarct Size

“…Rats were treated with vehicle and hearts were explanted without being subjected to IR (Sham), or were pretreated with water (control), PIO 2.5 mgkg −1 d −1 (PIO); TAK-491 3.0 mgkg −1 d −1 (TAK); or TAK+PIO and were subjected to IR. * p<0.01 vs. Cont; # p<0.01 vs. TAK+PIO fibroblats by blocking angiotensin II-induced transactivation of epidermal growth factor receptors [31]. Losartan prevents upregulation of ERK 1/2 in endothelial cells [32].…”

Helium-Neon Laser Irradiation Promotes the Proliferation and Migration of Human Epidermal Stem Cells In Vitro: Proposed Mechanism for Enhanced Wound Re-epithelialization

“…21 Immunoblotting was done using anti-extracellular signal-regulated kinase (ERK), anti-phospho-ERK (Santa Cruz Biotechnology, Santa Cruz, CA).…”