Helium-Neon Laser Irradiation Promotes the Proliferation and Migration of Human Epidermal Stem Cells In Vitro: Proposed Mechanism for Enhanced Wound Re-epithelialization

Abstract

Objective: The present study was conducted to investigate the effects of helium-neon (He-Ne) laser irradiation on the proliferation, migration, and differentiation of cultured human epidermal stem cells (ESCs). Background data: A He-Ne laser with a wavelength of 632.8 nm is known to have photobiological effects, and is widely used for accelerating wound healing; however, the cellular mechanisms involved have not been completely understood. Methods: The ESCs were prepared from human foreskin, and irradiated by using He-Ne laser at 632.8 nm with 2 J/cm 2 . The ESC proliferation, migration, and differentiation were examined by using XTT assay, scratch assay, and flow cytometry technology, respectively. The phosphorylation of extracellular signalregulated kinases (ERK) was analyzed by using Western blotting. Results: He-Ne laser irradiation markedly promoted cell proliferation and migration accompanied by an increase in the phosphorylation of ERK, but did not significantly influence cell differentiation. Conclusion: Our data indicated that photostimulation with a HeNe laser resulted in a significant increase in human ESC proliferation and migration in vitro, which might contribute, at least partially, to accelerated wound re-epithelialization by low-level laser therapy.

A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro

“…LLLT is known to stimulate the proliferation and differentiation of several types of cells, including fibroblasts and stem cells [8,9,19]. LLLT is a type of photomodulation that uses photons to modulate biological activity.…”

“…This therapy is widely applied in different branches of regenerative medicine, such as tissue regeneration [4] and dentistry, in which it is used to enhance the healing process [5]. Currently, LLLT is considered to be a reliable tool to enhance the proliferation of various cell lines [6][7][8] and stem cells [9]. Additionally, it has been reported that LLLT can increase skin pigmentation by stimulating melanocyte proliferation [2].…”

Phototherapy With LED Light Modulates Healing Processes in an In Vitro Scratch-Wound Model Using 3 Different Cell Types

“…Several groups showed that helium-neon laser irradiation stimulates the migration and cell proliferation of fibroblasts and keratinocytes with prospects of accelerated wound closure. 5,6 In vivo studies in rat skin wound models demonstrated a significant time reduction in different processes of wound healing, such as the inflammation phase, the reepithelization of wounds, and maturation phase. 7 Low-level laser therapy also showed beneficial effects in the healing of bones, nerves, and tendons.…”

The application of stem cells, visible and infrared light in regenerative medicine. Part 1

“…Из них 24, выполнен-ных на МСККМ [12,; 2 -на цельном кост-ном мозге (КМ) [53,54]; 9 -на МСКЖТ [17,31,[55][56][57][58][59][60][61]; 5 -на гематопоэтических СК (ГСК) [62][63][64][65][66]; 4 -на СК нервной ткани (НСК) [67][68][69][70]; 2 -на ЭСК [71,72]; 7 -на СК пульпы зуба (СКпЗ) [73][74][75][76][77][78] и перидонта [79]; 4 -на МСК пупочного канатика, печени, сердца и эпидермиса [49,[80][81][82] соответственно] и 2 -на необластах планарий Dugesia tigrina [83,84]. В анализ были включены 6 ра-бот, выполненных на фетальных (кальвариальных) клетках свода черепа крысы (ФКК) [85][86][87][88][89][90], счита-ющихся недифференцированными СК [91]; 8 -на сателлитных клетках поперечнополосатых мышц (СатК) [92][93][94]…”

“…при этом в обеих группах были получены как значительная, так и незначи-тельная прибавки скорости пролиферации. Следует отметить, что прибавку пролиферации около 100% можно получить, используя другие диапазоны длин волн, например, 560 нм [93], 940 нм [80], 1064 нм [40]. Из графика МСККМ рисунка видно, что мы можем получить различные значения прибавки пролиферации при одних и тех же значениях того или иного параметра, по-разному варьируя два дру-гих параметра.…”