Angiotensin II-induced depolarizations in hamster submandibular ganglion neurons.

Ikegami, Hideaki; Yamada, Takuya; Suzuki, Takashi

doi:10.2209/tdcpublication.41.29

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…In these neurons, the linearity and reversal potential for the net ANG-II-induced currents indicate involvement of a voltage-independent potassium conductance that contributes to resting membrane potential, often referred to as a leak conductance. Similar potassium-mediated responses to ANG II can be seen in rat bulbospinal (Li and Guyenet 1996) and median preoptic neurons (Bai and Renaud 1998) and hamster submandibular ganglion neurons (Ikegami et al 2000). In rat adrenal glomerulosa cells, ANG II suppresses a potassium conductance FIG.…”

Section: Discussionsupporting

confidence: 53%

Angiotensin AT₁-Receptors Depolarize Neonatal Spinal Motoneurons and Other Ventral Horn Neurons Via Two Different Conductances

Öz

Renaud

2002

Journal of Neurophysiology

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Angiotensin receptors are highly expressed in neonatal spinal cord. To identify their influence on neuronal excitability, we used patch-clamp recordings in spinal cord slices to assess responses of neonatal rat (5-12 days) ventral horn neurons to bath-applied angiotensin II (ANG II; 1 microM). In 14/34 identified motoneurons tested under current clamp, ANG II induced a slowly rising and prolonged membrane depolarization, blockable with Losartan (n = 5) and (Sar(1), Val(5), Ala(8))-ANG II (Saralasin, n = 4) but not PD123319 (1 microM each; n = 4). Under voltage clamp (V(H) -65 mV), 7/22 motoneurons displayed an ANG-II-induced tetrodotoxin-resistant inward current (-128 +/- 31 pA) with a similar time course, an associated reduction in membrane conductance and net current reversal at -98.8 +/- 3.9 mV. Losartan-sensitive ANG II responses were also evoked in 27/78 tested ventral horn "interneurons." By contrast with motoneurons, their ANG-II-induced inward current was smaller (-39.9 +/- 5.2 pA) and analysis of their I-V plots revealed three patterns. In eight cells, membrane conductance decreased with net inward current reversing at -103.8 +/- 4.1 mV. In seven cells, membrane conductance increased with net current reversing at -37.9 +/- 3.6 mV. In 12 cells, I-V lines remained parallel with no reversal within the current range tested. Intracellular dialysis with GTP-gamma-S significantly prolonged the ANG II effect in seven responsive interneurons and GDP-beta-S significantly reduced the ANG II response in four other cells. Peak inward currents were significantly reduced in all 13 responding neurons recorded in slices incubated in pertussis toxin (5 microgram/ml) for 12-18 h or in 12 neurons perfused with N-ethylmaleimide. Of 29 interneurons sensitive to pertussis toxin or N-ethylmaleimide treatment, 9 cells displayed a decrease in membrane conductance that reversed at -101.3 +/- 3.8 mV. In eight cells, membrane conductance increased and reversed at -38.7 +/- 3.4 mV. In 12 cells, the I-V lines remained parallel with no reversal within the current range tested, suggesting that both conductances are modulated by pertussis toxin-sensitive G proteins. These observations reveal a direct, G-protein-mediated depolarizing action of ANG II on neonatal rat ventral horn neurons. They also imply involvement of two distinct conductances that are differentially distributed among different cell types.

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Section: Discussionsupporting

confidence: 53%

Angiotensin AT₁-Receptors Depolarize Neonatal Spinal Motoneurons and Other Ventral Horn Neurons Via Two Different Conductances

Öz

Renaud

2002

Journal of Neurophysiology

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“…Ikegami et al (300) showed that ϳ70% of hamster submandibular ganglion neurons responded to ANG II with persistent depolarization. This effect is mediated by AT 1 receptor subtypes.…”

Section: Peripheral Nervous Systemmentioning

confidence: 99%

Physiology of Local Renin-Angiotensin Systems

Paul

Mehr²,

Kreutz³

2006

Physiological Reviews

1,502

1,371

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Since the first identification of renin by Tigerstedt and Bergmann in 1898, the renin-angiotensin system (RAS) has been extensively studied. The current view of the system is characterized by an increased complexity, as evidenced by the discovery of new functional components and pathways of the RAS. In recent years, the pathophysiological implications of the system have been the main focus of attention, and inhibitors of the RAS such as angiotensin-converting enzyme (ACE) inhibitors and angiotensin (ANG) II receptor blockers have become important clinical tools in the treatment of cardiovascular and renal diseases such as hypertension, heart failure, and diabetic nephropathy. Nevertheless, the tissue RAS also plays an important role in mediating diverse physiological functions. These focus not only on the classical actions of ANG on the cardiovascular system, namely, the maintenance of cardiovascular homeostasis, but also on other functions. Recently, the research efforts studying these noncardiovascular effects of the RAS have intensified, and a large body of data are now available to support the existence of numerous organ-based RAS exerting diverse physiological effects. ANG II has direct effects at the cellular level and can influence, for example, cell growth and differentiation, but also may play a role as a mediator of apoptosis. These universal paracrine and autocrine actions may be important in many organ systems and can mediate important physiological stimuli. Transgenic overexpression and knock-out strategies of RAS genes in animals have also shown a central functional role of the RAS in prenatal development. Taken together, these findings may become increasingly important in the study of organ physiology but also for a fresh look at the implications of these findings for organ pathophysiology.

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“…Some universities use commercialized or standardized tests while others make their own placement tests. Previous research has examined the relationship between placement tests and school grades (Otani et al ,2014;Sato et al ,2016;Ikegami, 2013;Obata, 2014) and documented a weak to moderate correlation between placement tests and grades, and that such correlations became weaker over time. Such research suggests there is some benefit in placing students into different class levels based on placement test results.…”

Section: Roles and Use Of Placement Tests In Japanese Universitiesmentioning

confidence: 99%

The IAFOR International Conference on Education – Hawaii 2024 Official Conference Proceedings

2024

IICE Official Conference Proceedings

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ChatGPT has grown in popularity among educators over the past few months. The way we teach and learn could be completely changed by the implementation of artificial intelligence (AI) technology in schools. Therefore, the main goal of this study is to determine in-depth public perceptions of ChatGPT use in Chinese educational programs, particularly for college students. Moreover, it also aims to examine how using ChatGPT affects writing performances. The foundation of this study is the technology acceptance model and the connectivism learning theory. Mixed methods will be used in questionnaires and in-depth interviews to identify the most prevalent issues with Chat GPT use on the university campus, and to suggest inclusive education initiatives that can support a secure and productive learning environment for WKU students. Besides, 200 respondents, including students, professors, and staff at WKU, will make up the sample. This study is beneficial for professors who teach writing at Wenzhou Kean University because it demonstrates how to use Chat GPT correctly to enhance students' own writing abilities. Furt hermore, the future application of Chat GPT in China's educational system will greatly benefit from the findings of this study.

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Angiotensin II-induced depolarizations in hamster submandibular ganglion neurons.

Cited by 6 publications

References 11 publications

Angiotensin AT₁-Receptors Depolarize Neonatal Spinal Motoneurons and Other Ventral Horn Neurons Via Two Different Conductances

Angiotensin AT₁-Receptors Depolarize Neonatal Spinal Motoneurons and Other Ventral Horn Neurons Via Two Different Conductances

Physiology of Local Renin-Angiotensin Systems

The IAFOR International Conference on Education – Hawaii 2024 Official Conference Proceedings

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Angiotensin II-induced depolarizations in hamster submandibular ganglion neurons.

Cited by 6 publications

References 11 publications

Angiotensin AT1-Receptors Depolarize Neonatal Spinal Motoneurons and Other Ventral Horn Neurons Via Two Different Conductances

Angiotensin AT1-Receptors Depolarize Neonatal Spinal Motoneurons and Other Ventral Horn Neurons Via Two Different Conductances

Physiology of Local Renin-Angiotensin Systems

The IAFOR International Conference on Education – Hawaii 2024 Official Conference Proceedings

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Product

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Angiotensin AT₁-Receptors Depolarize Neonatal Spinal Motoneurons and Other Ventral Horn Neurons Via Two Different Conductances

Angiotensin AT₁-Receptors Depolarize Neonatal Spinal Motoneurons and Other Ventral Horn Neurons Via Two Different Conductances