Angiotensin converting enzyme of Thalassophryne nattereri venom

Marques, Maria Elizabeth da Costa; Tenório, Humberto de Araújo; Santos, Claudio Wilian Victor dos; Santos, Daniel Moreira dos; Lima, Maria Elena de; Pereira, Hugo Juarez Vieira

doi:10.1016/j.ijbiomac.2016.06.051

Cited by 3 publications

(3 citation statements)

References 37 publications

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“…Further proteins identified as potentially involved in defense roles include a putative angiotensin-converting enzyme (ACE) regularly used in the processing of angiotensin II, an active vasoconstrictor. , Given the ACE proteins’ involvement in smooth muscle contraction, the enzyme may be involved in the overall mechanism of the secretion, with the gland itself contracting to release the contents. ACE proteins are also found within the venom of the sea snail Colubraria reticulata (false triton) and the venomous fish Thalassophryne nattereri . The fact that ACE is highly differentially expressed in the slime but angiotensin I and angiotensin II (the substrate and products of ACE) are not found in the slime may indicate that ACE is acting on proteins outside So.…”

Section: Discussionmentioning

confidence: 99%

“…ACE proteins are also found within the venom of the sea snail Colubraria reticulata (false triton) 60 and the venomous fish Thalassophryne nattereri. 61 The fact that ACE is highly differentially expressed in the slime but angiotensin I and angiotensin II (the substrate and products of ACE) are not found in the slime may indicate that ACE is acting on proteins outside So. lineolata.…”

Section: Slime-enriched Bioactive Peptides and Proteinsmentioning

confidence: 99%

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Quantitative Proteomic Analysis of the Slime and Ventral Mantle Glands of the Striped Pyjama Squid (Sepioloidea lineolata)

et al. 2020

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Cephalopods are known to produce an extensive range of secretions including ink, mucus, and venom. Sepiadariidae, a family of small, benthic bobtail squids, are notable for the high volume of viscous slime they emit when stressed. One species, Sepioloidea lineolata (striped pyjama squid), is covered with glands along the perimeter of the ventral mantle, and these structures are hypothesized to be the source of its slime. Using label-free quantitative proteomics, we analyzed five tissue types (dorsal and ventral mantle muscle, dorsal and ventral epithelium, and ventral mantle glands) and the slime from four individuals. In doing so, we were able to determine the relationship between the slime and the tissues as well as highlight proteins that were specifically identified within the slime and ventral mantle glands. A total of 28 proteins were identified to be highly enriched in slime, and these were composed of peptidases and protease inhibitors. Seven of these proteins contained predicted signal peptides, indicating classical secretion, with four proteins having no identifiable domains or similarity to any known proteins. The ventral mantle glands also appear to be the tissue with the closest overall proteomic composition to the slime; therefore, it is likely that the slime originates, at least in part, from these glands.

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Section: Discussionmentioning

confidence: 99%

Section: Slime-enriched Bioactive Peptides and Proteinsmentioning

confidence: 99%

Quantitative Proteomic Analysis of the Slime and Ventral Mantle Glands of the Striped Pyjama Squid (Sepioloidea lineolata)

et al. 2020

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“…The presence of an abundant transcript encoding a domain of unknown function (DM3421) that is shared with a group of venoms termed Natterins is intriguing. The fish Natterins are kininogenases that act on plasma kininogen to release kallidin/bradykinin, causing pain and oedema in the victim [ 37 , 38 ]. These enzymes are also plasminogen activators, causing activation of plasmin that breaks down fibrin clots.…”

Section: Discussionmentioning

confidence: 99%

Microexon gene transcriptional profiles and evolution provide insights into blood processing by the Schistosoma japonicum esophagus

DeMarco

Neves

et al. 2018

PLoS Negl Trop Dis

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BackgroundAdult schistosomes have a well-developed alimentary tract comprising an oral sucker around the mouth, a short esophagus and a blind ending gut. The esophagus is not simply a muscular tube for conducting blood from the mouth to gut but is divided into compartments, surrounded by anterior and posterior glands, where processing of ingested blood is initiated. Self-cure of rhesus macaques from a Schistosoma japonicum infection appears to operate by blocking the secretory functions of these glands so that the worms cease feeding and slowly starve to death. Here we use subtractive RNASeq to characterise the genes encoding the principal secretory products of S. japonicum esophageal glands, preparatory to evaluating their relevance as targets of the self-cure process.Methodology/Principal findingsThe heads and a small portion of the rear end of male and female S. japonicum worms were separately enriched by microdissection, for mRNA isolation and library construction. The sequence reads were then assembled de novo using Trinity and those genes enriched more than eightfold in the head preparation were subjected to detailed bioinformatics analysis. Of the 62 genes selected from the male heads, more than one third comprised MEGs encoding secreted or membrane-anchored proteins. Database searching using conserved motifs revealed that the MEG-4 and MEG-8/9 families had counterparts in the bird schistosome Trichobilharzia regenti, indicating an ancient association with blood processing. A second group of MEGs, including a MEG-26 family, encoded short peptides with amphipathic properties that most likely interact with ingested host cell membranes to destabilise them. A number of lysosomal hydrolases, two protease inhibitors, a secreted VAL and a putative natterin complete the line-up. There was surprisingly little difference between expression patterns in males and females despite the latter processing much more blood.Significance/ConclusionsThe mixture of approximately 40 proteins specifically secreted by the esophageal glands is responsible for initiating blood processing in the adult worm esophagus. They comprise the potential targets for the self-cure process in the rhesus macaque, and thus represent a completely new cohort of secreted proteins that can be investigated as vaccine candidates.

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Production, purification, characterization and application of a new halotolerant and thermostable endoglucanase of Botrytis ricini URM 5627

Silva

Albuquerque

Santos

et al. 2018

Bioresource Technology

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Angiotensin converting enzyme of Thalassophryne nattereri venom

Cited by 3 publications

References 37 publications

Quantitative Proteomic Analysis of the Slime and Ventral Mantle Glands of the Striped Pyjama Squid (Sepioloidea lineolata)

Quantitative Proteomic Analysis of the Slime and Ventral Mantle Glands of the Striped Pyjama Squid (Sepioloidea lineolata)

Microexon gene transcriptional profiles and evolution provide insights into blood processing by the Schistosoma japonicum esophagus

Production, purification, characterization and application of a new halotolerant and thermostable endoglucanase of Botrytis ricini URM 5627

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