Production, purification, characterization and application of a new halotolerant and thermostable endoglucanase of Botrytis ricini URM 5627

Silva, Tatielle P.; Albuquerque, Fabiana Sarmento de; Santos, Cláudio Willian V. dos; Franco, Marcelo; Caetano, Luiz Carlos; Pereira, Hugo Juarez Vieira

doi:10.1016/j.biortech.2018.09.022

Cited by 32 publications

(19 citation statements)

References 39 publications

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“…Thus, the present investigation reports a thermo‐halo‐tolerant endoglucanase. The desired thermo‐ and halotolerance/stability‐related properties of Cel M were discerned to be better than many previously reported proteins characterized as thermo‐halo‐tolerant endoglucanases from Thermobifida halotolerans (Yin et al, 2015), Botrytis ricini (Silva et al, 2018), Bacillus sp. (Aygan and Akiran, 2008), Icelandic hot spring isolate (Zarafeta et al, 2016), A. glaucus (Li et al, 2018), and Thermoanaerobacter tengcongensis (Liang et al, 2011; Table 2).…”

Section: Resultsmentioning

confidence: 73%

Biochemical characterization of a novel thermo‐halo‐tolerant GH5 endoglucanase from a thermal spring metagenome

Joshi

Kaushal

Singh

2021

Biotech & Bioengineering

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A novel endoglucanase gene, celM, was cloned from a thermal spring metagenome. The gene was expressed in Escherichia coli, and the protein was extracted and purified. The protein catalyzed the hydrolysis of amorphous cellulose in a wide range of temperatures, 30–95°C, with optimal activity at 80°C. It was able to tolerate high temperature (80°C) with a half‐life of 8 h. Its activity was eminent in a wide pH range of 3.0–11.0, with the highest activity at pH 6.0. The enzyme was tested for halostability. Any significant loss was not recorded in the activity of CelM after the exposure to salinity (3 M NaCl) for 30 days. Furthermore, CelM displayed a substantial resistance toward metal ions, denaturant, reducing agent, organic solvent, and non‐ionic surfactants. The amorphous cellulose, treated with CelM, was randomly cleaved, generating cello‐oligosaccharides of 2–5 degree of polymerization. Furthermore, CelM was demonstrated to catalyze the hydrolysis of cellulose fraction in the delignified biomass samples, for example, sweet sorghum bagasse, rice straw, and corncob, into cello‐oligosaccharides. Given that CelM is a thermo‐halo‐tolerant GH5 endoglucanase, with resistance to detergents and organic solvent, the biocatalyst could be of potential usefulness for a variety of industrial applications.

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Section: Resultsmentioning

confidence: 73%

Biochemical characterization of a novel thermo‐halo‐tolerant GH5 endoglucanase from a thermal spring metagenome

Joshi

Kaushal

Singh

2021

Biotech & Bioengineering

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“…Contudo, a maioria das endoglucanases apresentam temperaturas ótimas entre 50° a 70°C (Dashtban and Qin, 2009), a exemplo da endoglucanase isolado do Botrytis ricini, que apresentou temperatura ótima de 50°C. (SILVA et al, 2018).…”

Section: Características Das Endoglucanasesunclassified

AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

Nascimento¹,

Guedes²,

Bezerra³

et al. 2020

Pesquisa Científica E Tecnológica Em Microbiologia 3

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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“…Some of the endo- β-1,4-glucanases were directly isolated from microorganisms. For instance, a new thermostable and halotolerant endoglucanase was produced from Botrytis ricini URM 5627 [ 16 ]. In another research, a thermo-halotolerant and alkali stable GH6 endoglucanase from T. halotolerans were discovered that displays high catalytic activity in alkaline pH conditions and the presence of NaCl.…”

Section: Introductionmentioning

confidence: 99%

A novel high performance in-silico screened metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions

et al. 2020

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Background Lignocellulosic biomass, is a great resource for the production of bio-energy and bio-based material since it is largely abundant, inexpensive and renewable. The requirement of new energy sources has led to a wide search for novel effective enzymes to improve the exploitation of lignocellulose, among which the importance of thermostable and halotolerant cellulase enzymes with high pH performance is significant. Results The primary aim of this study was to discover a novel alkali-thermostable endo-β-1,4-glucanase from the sheep rumen metagenome. At first, the multi-step in-silico screening approach was utilized to find primary candidate enzymes with superior properties. Among the computationally selected candidates, PersiCel4 was found and subjected to cloning, expression, and purification followed by functional and structural characterization. The enzymes’ kinetic parameters, including Vmax, Km, and specific activity, were calculated. The PersiCel4 demonstrated its optimum activity at pH 8.5 and a temperature of 85 °C and was able to retain more than 70% of its activity after 150 h of storage at 85 °C. Furthermore, this enzyme was able to maintain its catalytic activity in the presence of different concentrations of NaCl and several metal ions contains Mg2+, Mn2+, Cu2+, Fe2+ and Ca2+. Our results showed that treatment with MnCl2 could enhance the enzyme’s activity by 78%. PersiCel4 was ultimately used for enzymatic hydrolysis of autoclave pretreated rice straw, the most abundant agricultural waste with rich cellulose content. In autoclave treated rice straw, enzymatic hydrolysis with the PersiCel4 increased the release of reducing sugar up to 260% after 72 h in the harsh condition (T = 85 °C, pH = 8.5). Conclusion Considering the urgent demand for stable cellulases that are operational on extreme temperature and pH conditions and due to several proposed distinctive characteristics of PersiCel4, it can be used in the harsh condition for bioconversion of lignocellulosic biomass.

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Production, purification, characterization and application of a new halotolerant and thermostable endoglucanase of Botrytis ricini URM 5627

Cited by 32 publications

References 39 publications

Biochemical characterization of a novel thermo‐halo‐tolerant GH5 endoglucanase from a thermal spring metagenome

Biochemical characterization of a novel thermo‐halo‐tolerant GH5 endoglucanase from a thermal spring metagenome

AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

A novel high performance in-silico screened metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions

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