Angiotensin-converting enzyme insertion/deletion gene polymorphisms and the risk of glioma in an Algerian population

Abstract

Introduction Just recently, it has been established that the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism is linked to the pathogenesis and to the evolution of human cancers. Therefore, the present study was concerned with the investigation of an eventual association between glioma and I/D polymorphism of the ACE gene. Methods The expression of ACE gene was detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis in 36 Algerian patients with glioma and 195 healthy controls. Results In glioma cases, allelic frequencies and genotypes distribution of the ACE I/D polymorphism were different from controls cases. ACE DD genotype were highly presented in glioma cases (63.9%) than controls (33.8%) and conferred 3.64-fold risk for predisposition in glioma cases (vs ID genotype, p<0.001). Recessive model (ACE II + ID genotypes vs DD) was associated with a 72% reduced risk of glioma (OR = 0.28, 95% CI: 0.13-0.60, p <0.001). Per copy D allele frequency was found higher in glioma cases (79.2%) than in controls (63.3 %), OR = 2.20, 95% CI: 1.20 - 4.03, p = 0.009. Conclusion The obtained data showed that the presence of the D allele might be a risk factor for the development of glioma. Further studies considering different ethnic groups with large samples are required to confirm this finding.

Association of Angiotensin I Converting Enzyme Insertion/287 bp Deletion Polymorphisms and Proliferative Prostatic Diseases among Lebanese Men

“…Other studies have found the I/D polymorphism to be associated with various cancers. For example, one study observed an association of the D allele with a 3-fold increased risk for glioma in an Algerian population [17], while a Polish study found the DD genotype to be associated with a 2-fold increased risk for multiple myeloma [18]. e I/D polymorphisms of the ACE gene have also been implicated in prostate cancer and breast cancer (reviewed in [19]).…”

The genetic polymorphisms of angiotensin converting enzyme insertion/deletion and glioma susceptibility: A meta-analysis

“…For the association between ACE I/D polymorphism and glioma, four studies including 1110 cases and 2335 controls were included for the analysis. Overall, the meta-analyses demonstrated no 8 China Chinese 800/800 Healthy subjects Pandith et al 9 India Indian 112/141 Healthy subjects Benenemissi et al 10 Algeria Algerian 36/195 Healthy subjects significant association between ACE I/D polymorphism and glioma risk. As shown in Figure 2(a), compared with ACE ID/II genotype, subjects with DD genotype showed a non-significant risk for glioma (OR 1.96; 95%CI 0.73 to 5.27; p = 0.18).…”

“…Thus, four studies 7 – 10 including 1110 cases and 1335 controls on ACE I/D polymorphism and glioma risk were included in this meta-analysis. All the studies were case-control studies and the distributions of the genotypes in the control populations were consistent with HWE in all the four studies ( Table 1 ).…”

“…Lian et al 8 also found that glioma cases had a significantly higher frequency of DD genotype than controls. Benenemissi et al 10 reported that ACE DD genotype was highly presented in glioma cases than controls and conferred 3.64-fold risk for predisposition in glioma cases compared with ID genotype. However, Li et al 7 reported that the ID genotype was associated with an increased risk of glioma compared with DD genotype.…”

“… 6 In the recent years, ACE I/D genetic polymorphisms has been concerned in the susceptibility of glioma. 7 – 10 However, the previous reports were inconsistent. Therefore, our present study aimed to perform a meta-analysis to assess the association between ACE I/D polymorphisms and glioma susceptibility.…”