Angiotensin-converting enzyme (ACE) inhibitory potential of standardizedMucuna pruriensseed extract

Abstract

Context: Mucuna pruriens Linn. (Fabaceae) is a tropical legume, traditionally used for controlling blood pressure. Inhibition of angiotensin-converting enzyme (ACE) is one of the successful strategies for controlling hypertension.Objective: The present study evaluated the ACE inhibition potential of the standardized extract of M. pruriens seeds. Materials and methods: Standardization of the extract and its fractions were carried out by RP-HPLC method [methanol and 1% v/v acetic acid in water (5:95 v/v)] using levodopa as a marker. The ACE inhibition activity of the extract and fractions was evaluated at different concentrations (20, 40, 60, 80, and 100 mg/mL) using the HPLC-DAD and the UV spectrophotometric method. The liberation of hippuric acid (HA) from hippuryl-L-histidyl-L-leucine (HHL) was estimated in the spectrophotometric method and RP-HPLC assay at 228 nm. Results: Methanol extract and aqueous fraction showed a maximum activity with IC 50 values of 38.44 ± 0.90 and 57.07 ± 2.90 mg/mL (RP-HPLC), and 52.68 ± 2.02 and 67.65 ± 2.40 mg/mL (spectrophotometry), respectively. Discussion: The study revealed that the aqueous extract contains the highest amount of levodopa. Eventually the methanol extract showed highest ACE inhibition activity except levodopa alone. It was further observed that the inhibition was altered with respect to the change in the content of levodopa in the extract. Thus, it can be assumed that levodopa may be responsible for the ACE inhibition activity of M. pruriens seeds. Conclusion: It can be concluded that M. pruriens seed is a potential ACE inhibitor can be explored further as an effective antihypertensive agent.

Purification of angiotensin‐converting enzyme from human plasma and investigation of the effect of some active ingredients isolated from Nigella sativa L. extract on the enzyme activity

“…At the same time, ACE inhibitors may cause hyperkalemia with the cause of aldosterone depletion (Hodsman et al, 1983;Warren & O'Connor, 1980 (Chaudhary, De, Bhadra, & Mukherjee, 2015). At the same time, ACE inhibitors may cause hyperkalemia with the cause of aldosterone depletion (Hodsman et al, 1983;Warren & O'Connor, 1980 (Chaudhary, De, Bhadra, & Mukherjee, 2015).…”

“…For example, the inhibitory effect of methanol extract and aqueous fraction of the Mucuna pruriens Linn. (Fabaceae) on ACE activity was investigated, and IC 50values were 52.68 ± 2.02 and 67.65 ± 2.40 mg/mL, respectively(Chaudhary, De, Bhadra, & Mukherjee, 2015). In another study, Peperomia pellucida L., Nasturtium officinale L., and Sesamum indicum L.showed in vitro inhibitory effect on ACE activity.…”

In Silico Investigation of Traditional Chinese Medicine for Potential Lead Compounds as SPG7 Inhibitors against Coronary Artery Disease

“…According to the information from TCM databases [27], 5-hydroxy- l -tryptophan is extracted from Mucuna pruriens seed, which has been indicated the function of antiproliferative effect [37], neuroprotective effect [38], control blood pressure [39]. In addition, 5-hydroxy- l -tryptophan is also the precursor to biosynthesis of 5-HT.…”

Inhibition of angiotensin converting enzyme by Rhazya stricta, Moringa peregrina and Achillea fragrantissima, used in traditional system of medicine in Arabian Peninsula: Implication in the management of hypertension

“…The anti-ACE activity of the three extracts were demonstrated by different inhibition potential as shown in (Pitt et al, 2000). Recent studies have shown the emergence of a number of plant-derived extracts with potential to provide lead molecules for the synthesis and development of new anti-hypertensive agents (Chaudhary et al, 2015;Gasparotto et al, 2011).…”