Angiotensin-Converting Enzyme (ACE) Inhibitory Activities of Protein Hydrolysates from Indonesian Sea Cucumbers

Dewi, Ariyanti Suhita; Patantis, Gintung; Fawzya, Yusro Nuri; Irianto, Hari Eko; Sa’diah, Siti

doi:10.1007/s10989-020-10035-5

Cited by 19 publications

(18 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dewi et al reported the hydrolysis of three species of under-utilized sea cucumbers from Lampung and Gorontalo provinces, using Alcalase, bromelain, or the combination of both enzymes, at hydrolysis conditions of pH 7, 45 °C, 24 h and enzyme/substrate ratio of 1% [496]. Results revealed that the Alcalase hydrolysates of H. atra contained the most active angiotensin I-converting enzyme inhibition activity with an IC50 value of 0.32 mg/mL [496].…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Use of Alcalase in the production of bioactive peptides: A review

Tacias-Pascacio

Morellon‐Sterling

Siar

et al. 2020

International Journal of Biological Macromolecules

205

114

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Use of Alcalase in the production of bioactive peptides: A review

Tacias-Pascacio

Morellon‐Sterling

Siar

et al. 2020

International Journal of Biological Macromolecules

205

114

View full text Add to dashboard Cite

show abstract

“…(Bordbar, Anwar & Saari, 2011). Various Indonesian sea cucumbers have been explored for their potential bioactivities (Adriansyah & Sulastri, 2014;Dewi, Patantis, Fawzya, Irianto, & Sa'diah, 2020;Khirzin, Sukarno, Yuliana, Fawzya, & Chasanah, 2015;Pangestuti & Arifin, 2018). However, many other local Indonesian sea cucumbers need further exploration of their biological activities related to their health benefit effects and possibilities as new sources of functional constituents.…”

Section: Introductionmentioning

confidence: 99%

Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

Fawzya¹,

Putra²,

Witarto³

et al. 2020

Squalen Bull. Marine Fisheries Postharvest Biotech.

Self Cite

View full text Add to dashboard Cite

Golden sea cucumber or locally known as “teripang emas” is one of Indonesia’s most popular sea cucumber and widely processed as functional food or supplement due to its bioactivities. The sea cucumber is often misidentified due to its morphological similarities with other Stichopus spp. This study aimed to identify the golden sea cucumber obtained from West Nusa Tenggara, Indonesia, by a molecular method and study the antioxidant activities of its collagen hydrolysates. The hydrolysates were produced by hydrolyzing acid collagen extract using neutrase for 30, 60, 120, 180, and 240 mins. The products were then analyzed for their degree of hydrolysis, peptide content, molecular weight distribution and radical scavenging activity by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. Results showed that hydrolysis for 180 mins was optimal in producing the highest peptide content, 12.79 ± 0.44 mg/mL, with a degree of hydrolysis (DH) of 55.2 ± 1.50%. However, the highest antioxidant activity (IC50 of 5.25 ± 0.15 mg/mL) was demonstrated after 60 mins hydrolysis with molecular weight (MW) ranged from less than 14.4 kDa to approximately 25 kDa. The hydrolysate might be categorized as a weak to moderate antioxidant. Based on the molecular identification, the golden sea cucumber had 99% similarities with Stichopus horrens and S. monotuberculatus.

show abstract

“…While the anti‐inflammatory mechanisms of bioactive peptides have not been completely elucidated, they seem to be associated with the modulation of transcription factors and the inhibition of pro‐inflammatory cytokines and chemokines. Sea cucumbers contain ACE‐inhibitory peptides that have anti‐hypertensive activity (Dewi et al., 2020; Ghanbari et al., 2015; Zhao et al., 2007). Their ACE inhibitory effect is mainly attributed to the binding ability of the peptides to active sites of ACE.…”

Section: Resultsmentioning

confidence: 99%

Anti‐inflammatory peptides and metabolomics‐driven biomarkers discovery from sea cucumber protein hydrolysates

Zhang

Wang

et al. 2021

Journal of Food Science

View full text Add to dashboard Cite

The hydrolysates from Apostichopus japonicus sea cucumber are an important source of nitrogen that may be added to foods. We evaluated the effect of A. japonicus hydrolysates on inflammation-associated leukocyte recruitment. The results revealed that leukocyte migration to the site of injury was significantly blocked by AJH-1 (<10 kDa), suggesting a protective effect against CuSO 4 -induced neuromast damage in a zebrafish model. Based on liquid chromatography/time-of-flight/mass spectrometry, and metabolomic analysis, the nine biomarker candidates in AJH-1 were Val, Ala-Pro-Arg, Gly-Lys, Asp propyl ester, Glu methyl ester, His butyl ester, Ile-Ala-Ala-Lys, Tyr-Lys, and Asn-Pro-Gly-Lys. We used molecular docking to predict the binding affinity and docked position of the peptides onto the angiotensin converting enzyme (ACE).All the identified peptides had adequate binding affinity toward ACE, especially peptides Ala-Pro-Arg and Gly-Lys. These peptides may be used in the development of therapeutic foods. Practical Application: The study revealed the anti-inflammatory properties of the fractionated sea cucumber protein hydrolysate (<10 kDa). The characteristic peptides may be used as functional ingredients in nutraceutical foods and beverages.

show abstract

Angiotensin-Converting Enzyme (ACE) Inhibitory Activities of Protein Hydrolysates from Indonesian Sea Cucumbers

Cited by 19 publications

References 29 publications

Use of Alcalase in the production of bioactive peptides: A review

Use of Alcalase in the production of bioactive peptides: A review

Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

Anti‐inflammatory peptides and metabolomics‐driven biomarkers discovery from sea cucumber protein hydrolysates

Contact Info

Product

Resources

About