Angiopoietin-like protein 2 may mediate the inflammation in murine mastitis through the activation of interleukin-6 and tumour necrosis factor-α

Wang, Jiying; Xiao, Hong-Bo; Sun, Zhi-Liang; Zhang, Dasheng

doi:10.1007/s11274-015-1873-7

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…Latest research has found that angiopoietin-like protein 2 (ANGPTL2) is highly expressed in human tissues of the intestine, fat, retina and heart. Furthermore, more research related to the significant high expression of ANGPTL2 in pulmonary cancer tissues, as well as distant metastasis in lung tissues and lymph nodes, has emerged, indicating that ANGPTL2 promotes pulmonary cancer cell proliferation and invasion[5-12]. The study of Teicher[13] also estimated its high expression in soft tissue sarcomas, implicating that ANGPTL2 may accelerate the progression of soft tissue sarcomas.…”

Section: Introductionmentioning

confidence: 99%

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

Sheng¹,

Chen²,

Tu³

et al. 2016

WJG

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AIMTo explore expression of angiopoietin-like protein 2 (ANGPTL2) and its effect on biological behavior such as proliferation and invasiveness in gastric cancer.METHODSWestern blotting was used to detect expression of ANGPTL2 in 60 human normal gastric tissues, 60 human gastric cancer tissues and gastric cell lines including GES-1, N87, SGC7901, BGC823 and PAMC82. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assay were used to detect the proliferation and invasive ability of gastric cancer cells.RESULTSCompared to normal tissues, ANGPTL2 protein levels were significantly upregulated in gastric tissues, and this level was closely correlated with gastric tumor grade, clinical stage and lymph node metastasis. Compared to GES-1 cells, ANGPTL2 mRNA and protein levels were significantly increased in gastric cancer cells including N87, SGC7901, BGC823 and PAMC82. The expression of ANGPTL2 in highly malignant gastric cancer cell lines BGC823 and PAMC82 was significantly higher than in low malignancy gastric cancer cell lines N87 and SGC7901. MTT and Transwell experiments indicated that the proliferation rate and invasive ability of stable overexpressed gastric cancer cells was faster than in cells transfected with Lv-NC and blank control cells, and the invasive ability of stable overexpressed gastric cancer cells was higher than that of cells transfected with Lv-NC and blank control cells.CONCLUSIONANGPTL2 contributed to proliferation and invasion of gastric cancer cells. In clinical treatment, ANGPTL2 may become a new target for treatment of gastric cancer.

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Section: Introductionmentioning

confidence: 99%

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

Sheng¹,

Chen²,

Tu³

et al. 2016

WJG

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“…ANGPTL2 is often associated with markers of inflammation such as CRP [ 64 , 65 ], IL-6 and TNF-α [ 4 , 66 ] and although it is not always clear which comes first, it is acknowledged that ANGPTL2 participates in a pro-inflammatory loop by being sensitive to inflammation and in turn, further promotes inflammatory pathways. In our study, ANGPTL2 methylation at CpG5 is inversely correlated with CRP.…”

Section: Discussionmentioning

confidence: 99%

Lower Methylation of the ANGPTL2 Gene in Leukocytes from Post-Acute Coronary Syndrome Patients

et al. 2016

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DNA methylation is believed to regulate gene expression during adulthood in response to the constant changes in environment. The methylome is therefore proposed to be a biomarker of health through age. ANGPTL2 is a circulating pro-inflammatory protein that increases with age and prematurely in patients with coronary artery diseases; integrating the methylation pattern of the promoter may help differentiate age- vs. disease-related change in its expression. We believe that in a pro-inflammatory environment, ANGPTL2 is differentially methylated, regulating ANGPTL2 expression. To test this hypothesis we investigated the changes in promoter methylation of ANGPTL2 gene in leukocytes from patients suffering from post-acute coronary syndrome (ACS). DNA was extracted from circulating leukocytes of post-ACS patients with cardiovascular risk factors and from healthy young and age-matched controls. Methylation sites (CpGs) found in the ANGPTL2 gene were targeted for specific DNA methylation quantification. The functionality of ANGPTL2 methylation was assessed by an in vitro luciferase assay. In post-ACS patients, C-reactive protein and ANGPTL2 circulating levels increased significantly when compared to healthy controls. Decreased methylation of specific CpGs were found in the promoter of ANGPTL2 and allowed to discriminate age vs. disease associated methylation. In vitro DNA methylation of specific CpG lead to inhibition of ANGPTL2 promoter activity. Reduced leukocyte DNA methylation in the promoter region of ANGPTL2 is associated with the pro-inflammatory environment that characterizes patients with post-ACS differently from age-matched healthy controls. Methylation of different CpGs in ANGPTL2 gene may prove to be a reliable biomarker of coronary disease.

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“…secreted from the infiltrating bone marrow-derived macrophages mediated the expression of pro-inflammatory cytokines, including TNF-α and IL-1β, to aggravate brain injury (Amadatsu et al, 2016). In addition, ANGPTL2 potentiates the inflammatory response in mastitis and paraquat induced acute lung damage in mice (Wang et al, 2015;Yang et al, 2018).…”

Section: Angptl2 In Acute Organ Injuriesmentioning

confidence: 99%

“…For example, elevated Angptl2 is found in a mouse model of ischemia‐reperfusion induced brain injury, while in an Angptl2 ‐/‐ and wildtype bone marrow chimeric mouse model, ANGPTL2 secreted from the infiltrating bone marrow‐derived macrophages mediated the expression of pro‐inflammatory cytokines, including TNF‐α and IL‐1β, to aggravate brain injury (Amadatsu et al, 2016). In addition, ANGPTL2 potentiates the inflammatory response in mastitis and paraquat induced acute lung damage in mice (Wang et al, 2015; Yang et al, 2018).…”

Section: Angptls and Inflammationmentioning

confidence: 99%

“…Second, multiple ANGPTLs are involved in regulating inflammation under physiological and pathological conditions. For example, ANGPTL 2, 3, and 4 participate in metabolic syndromes including atherosclerosis (Aryal et al, 2016; Farhat et al, 2013; Lu, 2020), adiposity, and systemic insulin resistance (Tabata et al, 2009); ANGPTL2 and 4 are pro‐inflammatory regulators in acute organ injuries such as brain/lung injury (Amadatsu et al, 2016; Guo et al, 2015; Li et al, 2015, 2019; Yang et al, 2018), mastitis (Wang et al, 2015; Xiao et al, 2018) and colonic inflammation (Phua et al, 2017). Furthermore, ANGPTLs 2, 3, 4, and 8 are involved in chronic organ/tissue injuries or repair such as abdominal aortic aneurysm (AAA) (Tazume et al, 2012), chronic kidney disease (CKD) (Lee et al, 2013; Morinaga et al, 2016), diabetic nephropathy (DN) (Huang et al, 2019; Yang et al, 2017), diabetic retinopathy (Wang et al, 2018), and in tissue repair following myocardial infarction (Cho et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Emerging roles of angiopoietin‐like proteins in inflammation: Mechanisms and potential as pharmacological targets

Yang

Song

Niu

et al. 2021

Journal Cellular Physiology

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Angiopoietin-like proteins (ANGPTLs), a family of eight secreted glycoproteins termed ANGTPL1-8, are involved in angiogenesis, lipid metabolism, cancer progression, and inflammation. Their roles in regulating lipid metabolism have been intensively studied, as some ANGPTLs are promising pharmacological targets for hypertriglyceridemia and associated cardiovascular disease. Recently, the emerging roles of ANGPTLs in inflammation have attracted great attention. First, elevated levels of multiple circulating ANGPTLs in inflammatory diseases make them potential disease biomarkers. Second, multiple ANGPTLs regulate acute or chronic inflammation via various mechanisms, including triggering inflammatory signaling through their action as ligands for integrin or forming homo-/hetero-oligomers to regulate signal transduction via extra-or intracellular mechanisms. As dysregulation of the inflammatory response is a critical trigger in many diseases, understanding the roles of ANGPTLs in inflammation will aid in drug/therapy development. Here, we summarize the roles, mechanisms, and potential therapeutic values for ANGPTLs in inflammation and inflammatory diseases.

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Angiopoietin-like protein 2 may mediate the inflammation in murine mastitis through the activation of interleukin-6 and tumour necrosis factor-α

Cited by 5 publications

References 19 publications

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

Lower Methylation of the ANGPTL2 Gene in Leukocytes from Post-Acute Coronary Syndrome Patients

Emerging roles of angiopoietin‐like proteins in inflammation: Mechanisms and potential as pharmacological targets

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