Angionorm Complex Herbal Preparation Constituents Identified by High Performance Liquid Chromatography-Mass Spectrometry

Стручков, Петр Андреевич; Мельников, Е. С.; Beloborodov, V. L.; Kolkhir, V. K.; Voskoboinikova, I. V.

doi:10.1007/s11094-019-01937-w

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…We observed a mixture of substances in each sample since the compounds with similar properties could not be completely separated on the TLC plate. We have carried out a preliminary identification of the substances in TLC spot samples using literature data (Fu and Wang, 2015) and the results of our previous studies (Adamov et al ., 2020, Struchkov et al ., 2019). We assumed the presence of apigenin, genistein, luteolin, kaempferol and formononetin in all the studied extracts.…”

Section: Resultsmentioning

confidence: 99%

Exploring the anti‐α‐amylase activity of flavonoid aglycones in fabaceae plant extracts: a combined MALDI‐TOF‐MS and LC–MS/MS approach

Филимонова

Мельников

Kaufmann

et al. 2023

Int J of Food Sci Tech

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A combination of TLC-bioautography, MALDI-TOF-MS and LC-MS/MS methods was used to identify flavonoids with anti-α-amylase activity in extracts of Lathyrus pratensis L. (herb), L. polyphillus L. (fruits), Thermopsis lanceolata R. Br. (herb) and S. japonica L. (buds). After the TLC-autobiography assay, substances with anti-amylase activity were identified by MALDI-TOF-MS followed by confirmation of the result by LC-MS/MS. Results of the study revealed that the flavonoids apigenin, luteolin, formononetin, genistein and kaempferol display marked anti-α-amylase activity. Formononetin showed the largest activity. Compared with LC-MS/MS, MALDI-TOF-MS is a quick and convenient method; results can be obtained within minutes; and only minor sample amounts are required which allows us to analyse mixtures of substances without preliminary separation. However, the inability to distinguish between isomers is the main limitation of the method.

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Section: Resultsmentioning

confidence: 99%

Exploring the anti‐α‐amylase activity of flavonoid aglycones in fabaceae plant extracts: a combined MALDI‐TOF‐MS and LC–MS/MS approach

Филимонова

Мельников

Kaufmann

et al. 2023

Int J of Food Sci Tech

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“…Hawthorn berry, as the important medicinal health food (MHF), has been proved hypoglycemic effect due to the presence of some natural α‐glucosidase inhibitors including quercetin, hyperoside, catechin, rutin and glycoside compounds (Edwards et al., 2012; Natic et al., 2019; Struchkov et al., 2019). However, the exact therapeutic material basis remained ambiguous.…”

Section: Discussionmentioning

confidence: 99%

Hawthorn polyphenols, D‐chiro‐inositol, and epigallocatechin gallate exert a synergistic hypoglycemic effect

et al. 2021

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Hawthorn berry has been proved hypoglycemic effect due to the presence of some α‐glucosidase inhibitors. Herein, screening and identifying of α‐glucosidase inhibitors from hawthorn berry were conducted, and the results showed polyphenols mainly containing quercetin (74.58%) and hyperioside (9.58%) were responsible for its bioactivity. In order to enhance the hypoglycemic effect, the combined glucose‐lowering complex (DEH) consisting of hawthorn polyphenols, D‐chiro‐inositol (DCI), and epigallocatechin gallate (EGCG) was prepared, where three ingredients exerted the synergistic hypoglycemic effect to enhance glucose consumption and glycogen levels and inhibit hepatic gluconeogenesis in IR‐HepG2 cells. In STZ/HFD‐induced mice, DEH effectively improved insulin resistance, reduced fasting blood glucose (FBG) and hepatic gluconeogenesis, and increased hepatic glycogen synthesis and storage via down‐regulation of PI3K/Akt/FOXO1‐mediated PEPCK and G6Pase and up‐regulation of PI3K/Akt/GSK3‐mediated GS activation in the liver. In summary, these findings indicate that DEH is a potential novel strategy for the treatment of type 2 diabetes. Practical applications Glucose‐lowering complex (DEH) with superior hypoglycemic effect, can effectively improve the insulin resistance, reduce the fasting blood glucose, hepatic gluconeogenesis and increase the hepatic glycogen synthesis and storage in mice, which represents a potential novel strategy for the treatment of type 2 diabetes.

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Angionorm Complex Herbal Preparation Constituents Identified by High Performance Liquid Chromatography-Mass Spectrometry

Cited by 2 publications

References 16 publications

Exploring the anti‐α‐amylase activity of flavonoid aglycones in fabaceae plant extracts: a combined MALDI‐TOF‐MS and LC–MS/MS approach

Exploring the anti‐α‐amylase activity of flavonoid aglycones in fabaceae plant extracts: a combined MALDI‐TOF‐MS and LC–MS/MS approach

Hawthorn polyphenols, D‐chiro‐inositol, and epigallocatechin gallate exert a synergistic hypoglycemic effect

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