Angiogenic property of silk fibroin scaffolds with adipose-derived stem cells on chick chorioallantoic membrane

Abstract

Angiogenesis is a crucial step in tissue regeneration and repair. Biomaterials that allow or promote angiogenesis are thus beneficial. In this study, angiogenic properties of salt-leached silk fibroin (SF) scaffolds seeded with human adipose stem cells (hADSCs) were studied using chick chorioallantoic membrane (CAM) as a model. The hADSC-seeded SF scaffolds (SF-hADSC) with the porosity of 77.34 ± 6.96% and the pore diameter of 513.95 ± 4.99 µm were implanted on the CAM of chick embryos that were on an embryonic day 8 (E8) of development. The SF-hADSC scaffolds induced a spoke-wheel pattern of capillary network indicative of angiogenesis, which was evident since E11. Moreover, the ingrowth of blood vessels into the scaffolds was seen in histological sections. The unseeded scaffolds induced the same extent of angiogenesis later on E14. By contrast, the control group could not induce the same extent of angiogenesis. In vitro cytotoxicity tests and in vivo angioirritative study reaffirmed the biocompatibility of the scaffolds. This work highlighted that the biocompatible SF-hADSC scaffolds accelerate angiogenesis, and hence they can be a promising biomaterial for the regeneration of tissues that require angiogenesis.

Silk fibroin scaffolds: A promising candidate for bone regeneration

“…Usually, SF scaffolds used for bone regeneration exhibit high porosity and thus allow the vessel ingrowth, which can be improved by pre-seeded cells before implantations. Watchararot et al (2021) seeded human adipose-derived stem cells (hADSC) onto SF scaffolds and implanted them into chick chorioallantoic membrane with the pore diameter of 513.96 ± 4.99 μm and porosity of 77.34 ± 6.96%. A capillary network of spoke-wheel pattern was induced by the seeded scaffolds 3 weeks earlier than unseeded scaffolds, indicating an early angiogenesis.…”

Frozen bean curd-inspired xenogeneic acellular dermal matrix with triple pretreatment approach of freeze–thaw, laser drilling and ADSCs pre-culture for promoting early vascularization and integration

“…ADSCs served as usual tools for vascularization in tissue engineering because of their convenient management, ability to self-renew and differentiate into various cell types, and secretion of angiogenic factors [ 31 , 32 ]. ADSCs-seeded silk fibroin scaffolds or hydrogels that accelerated angiogenesis were reported [ 33 , 34 ]. Our previous study also reported that ADSCs-seeded-PADM promoted pro-vascular gene expression and increased vascular infiltration [ 35 ].…”

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

“…These factors are released from different stem cell types such as EPCs, MSCs, and peripheral blood mononuclear cells and have been typically used for angiogenesis evaluations in several preclinical and clinical studies [ 262 ]. In response to the gradients of angiogenic factors, EPCs, MSCs, and peripheral blood mononuclear cells are recruited from different tissues, especially bone marrow, and accommodated the injured sites [ 263 ].…”