Angiogenic potential of co-spheroids of neural stem cells and endothelial cells in injectable gelatin-based hydrogel

Han, Hao-Wei; Hou, Yung-Te; Hsu, Shan‐hui

doi:10.1016/j.msec.2019.01.089

Cited by 24 publications

(20 citation statements)

References 46 publications

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“… 143 Similarly, Han and colleagues reported that adult mouse NSCs and bovine carotid artery endothelial cells cultured on chitosan-hyaluronan substrates readily formed co-spheroids. 124 Unlike the previous study, co-spheroids showed an increase in both neurogenesis and gliogenesis compared to NSC-only neurospheres. The source of the discrepancy between the two studies is unclear but likely due to the use of different cell types.…”

Section: Modeling the Interaction Between Neural Stem Cells And Endotcontrasting

confidence: 66%

“…The source of the discrepancy between the two studies is unclear but likely due to the use of different cell types. 124,143 In addition, co-spheroids exhibited the growth of capillary-like structures and enhanced expression of angiogenesis markers when cultured in bFGF-bound gelatin-based hydrogels. 124 These data demonstrated the angiogenic potential of these co-spheroids.…”

Section: Modeling the Interaction Between Neural Stem Cells And Endotmentioning

confidence: 99%

“…Given the similarities of endothelial cell genomes and secretomes, several in vitro studies have investigated the interaction between NSCs/NPCs and nonbrain endothelial cells, including HUVECs, 76 dermal microvascular endothelial cells, 121 human endothelial colony-forming cell-derived endothelial cells (ECFC-ECs), 123 and even bovine carotid artery endothelial cells. 124 Regardless of the origin, endothelial cells will provide paracrine and juxtacrine signals that influence NSC/NPC behavior in in vitro models.…”

Section: Modeling the Interaction Between Neural Stem Cells And Endotmentioning

confidence: 99%

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Bioengineering the neurovascular niche to study the interaction of neural stem cells and endothelial cells

Winkelman

Koppes

et al. 2021

APL Bioengineering

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The ability of mammalian neural stem cells (NSCs) to self-renew and differentiate throughout adulthood has made them ideal to study neurogenesis and attractive candidates for neurodegenerative disease therapies. In the adult mammalian brain, NSCs are maintained in the neurovascular niche (NVN) where they are found near the specialized blood vessels, suggesting that brain endothelial cells (BECs) are prominent orchestrators of NSC fate. However, most of the current knowledge of the mammalian NVN has been deduced from nonhuman studies. To circumvent the challenges of in vivo studies, in vitro models have been developed to better understand the reciprocal cellular mechanisms of human NSCs and BECs. This review will cover the current understanding of mammalian NVN biology, the effects of endothelial cell-derived signals on NSC fate, and the in vitro models developed to study the interactions between NSCs and BECs.

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Section: Modeling the Interaction Between Neural Stem Cells And Endotcontrasting

confidence: 66%

Section: Modeling the Interaction Between Neural Stem Cells And Endotmentioning

confidence: 99%

Section: Modeling the Interaction Between Neural Stem Cells And Endotmentioning

confidence: 99%

See 1 more Smart Citation

Bioengineering the neurovascular niche to study the interaction of neural stem cells and endothelial cells

Winkelman

Koppes

et al. 2021

APL Bioengineering

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“…We previously demonstrated 11 MSCs phenotype is dramatically changed when culture condition shifts from 2D to 3D and also depend on the approaches utilized to generate the spheroids (35), while the differences in PDGR-seems to be related to different to the presence of mineral addictives.…”

Section: Discussionmentioning

confidence: 99%

Culture of porcine Vascular Wall - Mesenchymal Stem Cells on 3D biodegradable highly porous mineral doped Poly (α-hydroxy) acids scaffolds

Forni¹,

Bernardini

Zamparini³

et al. 2019

Preprint

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Background Vascularization is a crucial factor when approaching any engineered tissue. Hybrid biomaterials composed of biodegradable synthetic polymers and inorganic materials showed suitable properties for biomedical application. Being Vascular Wall – Mesenchymal Stem Cells derived from pig thoracic aorta (pVW-MSCs) an excellent in vitro model to study vascular remodelling due to their strong angiogenic attitude, the aim of the present study was to demonstrate the angiogenic potential of experimental highly porous scaffolds based on polylactic acid (PLA) or poly-e-caprolactone (PCL) doped with calcium silicates (CaSi) and dicalcium phosphate dehydrated (DCPD), namely PLA-10CaSi-10DCPD and PCL-10CaSi-10DCPD. Results The results obtained clearly demonstrated that the cells colonized the scaffolds and were metabolically active. Moreover cells, grown in these 3D systems, showed the typical gene expression profile they have in control 2D culture, although with some main quantitative differences. DAPI staining and immunofluorescence assay confirmed cellular presence on both scaffolds, however pVW-MSCs cultured in PLA-10CaSi-10DCPD showed an individual cellular growth, in contrast, in PCL-10CaSi-10DCPD scaffolds pVW-MSCs grew in spherical clusters. Conclusion In conclusion, the property of the experimental scaffolds demonstrated the suitability of the scaffolds to be colonized by vascular stem cells for new vessels formation and a potential application in tissue regeneration for biomedical purpose.

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“…Gelatin is a natural hydrophilic polymer well known for its use in different areas of tissue engineering including skin, neuron, cartilage, and bone [10][11][12][13]. Rising interesting in gelatin-based biomaterials is because of the fact that they are biocompatible, non-antigenic, and biodegradable [14,15].…”

Section: Introductionmentioning

confidence: 99%

Methacrylation increase growth and differentiation of primary human osteoblasts for gelatin hydrogels

et al. 2020

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The role of gelatin methacrylate hydrogels with varying degrees of methacrylation (69% and 84%) was accessed with FTIR, NMR, microCT, and subsequent exposure to human osteoblasts. The cells responded positively to the degree of methacrylation and showed attachment, growth, and proliferated on both hydrogels. The cell reacted differently to the degree of methacrylation with higher proliferation on higher substitution; however, cell differentiation behavior was improved for less substitution. The secretion of late osteogenic markers (osteoprotegerin (OPG), osteopontin (OPN), and osteocalcin (OCN)) and angiogenic factor vascular endothelial growth factor (VEGF) was increased for gelatin methacrylate hydrogels with 69% degree of methacrylation and thus would be the better candidate for future bone regenerative applications amongst the three tested hydrogels.

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Angiogenic potential of co-spheroids of neural stem cells and endothelial cells in injectable gelatin-based hydrogel

Cited by 24 publications

References 46 publications

Bioengineering the neurovascular niche to study the interaction of neural stem cells and endothelial cells

Bioengineering the neurovascular niche to study the interaction of neural stem cells and endothelial cells

Culture of porcine Vascular Wall - Mesenchymal Stem Cells on 3D biodegradable highly porous mineral doped Poly (α-hydroxy) acids scaffolds

Methacrylation increase growth and differentiation of primary human osteoblasts for gelatin hydrogels

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