Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway

Yeh, Tzu Shao; Hsu, Chin‐Lin; Yang, Suh‐Ching; Hsu, Mei Chich; Liu, Jen‐Fang

doi:10.1186/1472-6882-14-144

Cited by 12 publications

(8 citation statements)

References 32 publications

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“…This strongly stained myotubes and nuclei within. To determine the hypertrophy index, myotube diameter was determined according to Yeh et al [21]. Cultured cells were photographed using 10x objective lens and NIH-Image J software was used to measure myotube diameter.…”

Section: Methodsmentioning

confidence: 99%

Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation

Obradović

Krstić

Кукољ

et al. 2016

Mediators of Inflammation

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Interleukin 17 (IL-17) is a cytokine with pleiotropic effects associated with several inflammatory diseases. Although elevated levels of IL-17 have been described in inflammatory myopathies, its role in muscle remodeling and regeneration is still unknown. Excessive extracellular matrix degradation in skeletal muscle is an important pathological consequence of many diseases involving muscle wasting. In this study, the role of IL-17 on the expression of matrix metalloproteinase- (MMP-) 9 in myoblast cells was investigated. The expression of MMP-9 after IL-17 treatment was analyzed in mouse myoblasts C2C12 cell line. The increase in MMP-9 production by IL-17 was concomitant with its capacity to inhibit myogenic differentiation of C2C12 cells. Doxycycline (Doxy) treatment protected the myogenic capacity of myoblasts from IL-17 inhibition and, moreover, increased myotubes hypertrophy. Doxy blocked the capacity of IL-17 to stimulate MMP-9 production by regulating IL-17-induced ERK1/2 MAPK activation. Our results imply that MMP-9 mediates IL-17's capacity to inhibit myoblast differentiation during inflammatory diseases and indicate that Doxy can modulate myoblast response to inflammatory induction by IL-17.

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Section: Methodsmentioning

confidence: 99%

Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation

Obradović

Krstić

Кукољ

et al. 2016

Mediators of Inflammation

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“…After these different treatments, the cells were fixed and stained according to the protocol described in the wound healing assay section. Morphometric parameters of the formed myotubes were determined using a light microscope and a digital camera system (Olympus BX51, Japan) as previously described [23]. Namely, C2C12 cells were cultured in different media in 6-well plates; 10 myotubes per well were photographed and measured, or all the observed myotubes for the given treatment if their number was <10.…”

Section: Myogenic Differentiation and Morphometric Analysismentioning

confidence: 99%

Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation

Stančić

Drvenica

Bugarski

et al. 2020

ARCH BIOL SCI BELGRA

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Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of the SC experimental model; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 ?M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1?) at a concentration of 10 ?M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 451-03-68/2020-14/200015]

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“…It was reported that ferulic acid could inhibit platelet aggregation, increase coronary blood flow, relax or stimulate smooth muscle and possesses anti-arrhythmic effect [17]. More recent study found that phthalides inhibited the formation of reactive oxygen species and lipid peroxidation, and enhanced the cellular resistance to hydrogen peroxide-induced oxidative damage [18].…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Ferulic Acid and Phthalides of Angelica Sinensis Based on UPLC-Q-TOF/MS

Wei

Huang

2015

Molecules

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Abstract:The radix of Angelica sinensis (AS) is one of the most commonly used as a herbal medicine. To investigate the geoherbalism and quality evaluation of AS, an ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF/MS) method was established to analyze and identify ferulic acid and phthalides in AS. The results showed that among samples collected in four regions, the relative contents of ferulic acid and phthalides were highest in samples collected in Gansu, and the samples from the four different regions were apparently classified into four groups. Meanwhile, the relative content in non-fumigated root was higher than after sulfur-fumigation and the sulfur-fumigated and non-fumigated samples were obviously divided into two groups by PCA. The paper establishes a systematic and objective evaluation system to provide a scientific basis for evaluating the quality of AS.

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Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway

Cited by 12 publications

References 32 publications

Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation

Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation

Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation

Simultaneous Determination of Ferulic Acid and Phthalides of Angelica Sinensis Based on UPLC-Q-TOF/MS

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