Anfärbung der inneren Grenzmembran der Netzhaut mit Brilliant Blau

Höing, Anna; Remy, Matthias; Dirisamer, Martin; Priglinger, SG; Cl, Schönfeld; Haritoglou, Christos

doi:10.1055/s-0031-1273351

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…In humans, BBG caused adequate ILM staining in an iso-osmolar solution of 0.25 mg/ml (0.025%) with good clinical results and no signs of toxicity in multifocal electroretinography in most studies [6,11,19,20]. However, we recently demonstrated that subretinal migration of the dye may cause atrophic changes of the retinal pigment epithelium, and therefore, we strongly suggest avoidance of BBG exposure of the RPE during chromovitrectomy [21].…”

Section: Discussionmentioning

confidence: 99%

Staining Properties of Brilliant Blue Depending on Different Incubation Times and Solvents in Humans

Rodrigues¹,

Maia²,

Penha³

et al. 2013

Ophthalmologica

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Purpose: Intravitreous injection of vital dyes, e.g. brilliant blue (BBG), promotes better visualization of the internal limiting membrane (ILM). This paper investigates the staining properties of BBG depending on different incubation times and 2 types of solvents - 5% glucose (GL) or saline solution - in a prospective study in patients. Methods: This paper investigates various aspects of BBG in various methods. An interventional prospective study was conducted in patients to examine the binding properties of the blue dye diluted in either saline or 5% GL to epiretinal membranes (ERMs) and ILMs. Forty-nine consecutive patients older than 18 years scheduled for macular ERM, vitreomacular traction and macular hole surgeries were prospectively recruited. The primary outcomes of this study were the degree of ILM and ERM staining. The secondary outcomes of the study were the need of reinjection of BBG or any other dye, the ability of BBG to stain the vitreous, and frequency of complications. The staining of the ILM and ERM were graded as no staining, little, moderate or strong staining. Results: There was no correlation between age (p = 0.32) or gender (p = 0.33) in the staining affinity of BBG to either the ILM or ERM. BBG may be an appropriate staining agent for the ILM in the majority (82%) of surgeries; however, in approximately half of the cases (45%) surgeons considered BBG not enough for ERM coloring and visualization. There is a tendency of BBG to stain the ILM better when saline solution is used compared to GL 5%; however, this was not statistically significant (p = 0.64). There was no difference in the staining efficacy of BBG to the ERMs by either solution (p = 0.70), despite the low staining affinity. Conclusion: BBG became the state-of-the-art dye for ILM identification. Differences in staining properties may imply that BBG should not be considered as first-line stain for ERM surgery. BBG is effective in ILM staining in over 80% of macular hole surgeries.

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Section: Discussionmentioning

confidence: 99%

Staining Properties of Brilliant Blue Depending on Different Incubation Times and Solvents in Humans

Rodrigues¹,

Maia²,

Penha³

et al. 2013

Ophthalmologica

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“…At the end, they concluded that BBG sufficiently and selectively stained the internal limiting membrane (ILM). Additionally, there was no retinal toxicity or side effects associated with the BBG, and the safety of long-term ingestion should be evaluated in a larger patient series and in a longer follow-up [58]. Based on these preliminary studies and molecular structural similarities among BBG and other Brilliant Blue food dyes, Franke and collaborators investigated purinergic precipitates in microglial activation in the rat nucleus accumbens (NAc) in vivo based on extracellular ATP release after brain injury.…”

Section: Brilliant Blue Dyes In Vivo Assaysmentioning

confidence: 99%

Brilliant Blue Dyes in Daily Food: How Could Purinergic System Be Affected?

Ferreira

Faria

Ferreira

et al. 2016

International Journal of Food Science

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Dyes were first obtained from the extraction of plant sources in the Neolithic period to produce dyed clothes. At the beginning of the 19th century, synthetic dyes were produced to color clothes on a large scale. Other applications for synthetic dyes include the pharmaceutical and food industries, which are important interference factors in our lives and health. Herein, we analyzed the possible implications of some dyes that are already described as antagonists of purinergic receptors, including special Brilliant Blue G and its derivative FD&C Blue No. 1. Purinergic receptor family is widely expressed in the body and is critical to relate to much cellular homeostasis maintenance as well as inflammation and cell death. In this review, we discuss previous studies and show purinergic signaling as an important issue to be aware of in food additives development and their correlations with the physiological functions.

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Investigating retinal toxicity of a lutein-based dye in a model of isolated and perfused bovine retina

Mueller

Krupp

Schnichels

et al. 2019

Graefes Arch Clin Exp Ophthalmol

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Anfärbung der inneren Grenzmembran der Netzhaut mit Brilliant Blau

Abstract: Brilliant blue provides a sufficient and selective staining of the ILM. No retinal toxicity or adverse effects related to the dye were observed in this study. The long-term safety of this dye will have to be evaluated in larger patient series and a longer follow-up.

Cited by 5 publications

References 12 publications

Staining Properties of Brilliant Blue Depending on Different Incubation Times and Solvents in Humans

Staining Properties of Brilliant Blue Depending on Different Incubation Times and Solvents in Humans

Brilliant Blue Dyes in Daily Food: How Could Purinergic System Be Affected?

Investigating retinal toxicity of a lutein-based dye in a model of isolated and perfused bovine retina

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