Brilliant Blue Dyes in Daily Food: How Could Purinergic System Be Affected?

Ferreira, L. G.; Faria, Robson Xavier; Ferreira, Natiele Carla da Silva; Soares‐Bezerra, Rômulo José

doi:10.1155/2016/7548498

Cited by 25 publications

(18 citation statements)

References 93 publications

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“…Importantly, BB-FCF is rapidly excreted from the body and does not seem to display (serious) side-effects either [25]. However, similar to other pharmacological Panx1 inhibitors, the Panx1 specificity of BB-FCF has recently been called into question by studies showing that high concentrations of the dye can directly inhibit P2X receptors [31], and it can thus not be excluded that the in vivo effects of BB-FCF on hemostasis may be partly driven by inhibition of P2X7 receptors expressed in the endothelium. This further emphasizes the need for development of specific Panx1 channel blockers.…”

Section: Blocking Panx1 Channels Delays Hemostasis and Thrombosis In mentioning

confidence: 99%

Selective inhibition of Panx1 channels decreases hemostasis and thrombosis in vivo

Molica

Meens

Pelli

et al. 2019

Thrombosis Research

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Background: Hemostasis is a tightly regulated physiological process to rapidly induce hemostatic plugs at sites of vascular injury. Inappropriate activation of this process may lead to thrombosis, i.e. pathological blood clot formation in uninjured vessels or on atherosclerotic lesions. ATP release through Pannexin1 (Panx1) membrane channels contributes to collagen-induced platelet aggregation in vitro. Objective: To investigate the effects of genetic and pharmacological inhibition of Panx1 on hemostasis and thrombosis in vivo. Results: Bleeding time after tail clipping was increased by 2.5-fold in Panx1 −/− mice compared to wild-type controls, suggesting that Panx1 deficiency impairs primary hemostasis. Wire myography on mesenteric arteries revealed diminished vasoconstriction in response to phenylephrine or U446619 in Panx1 −/− mice. Mice with platelet-specific deletion of Panx1 (Panx1 PDel) displayed 2-fold longer tail bleeding times than Panx1 fl/fl controls. Moreover, venous thromboembolism (VTE) after injection of collagen/epinephrine in the jugular vein was reduced in Panx1 −/− and Panx1 PDel mice. Panx1 PDel mice also showed reduced FeCl 3-induced thrombosis in mesenteric arteries. BrilliantBlue-FCF, a Panx1 channel inhibitor, decreased collagen-induced platelet aggregation in vitro, increased tail bleeding time and reduced VTE in wild-type mice. Furthermore, we developed a specific Panx1 blocking antibody targeting a Panx1 extracellular loop, which reduced ATP release from platelets in vitro. Treating wild-type mice with this antibody increased tail bleeding time and decreased VTE compared to control antibody. Conclusions: Panx1 channel deletion or inhibition diminishes clot formation during hemostasis and thrombosis in vivo. Blocking Panx1 channels may be an attractive strategy for modulating platelet aggregation in thrombotic disease.

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Section: Blocking Panx1 Channels Delays Hemostasis and Thrombosis In mentioning

confidence: 99%

Selective inhibition of Panx1 channels decreases hemostasis and thrombosis in vivo

Molica

Meens

Pelli

et al. 2019

Thrombosis Research

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“…Brilliant blue G, also known as Coomassie brilliant blue, is used as a food additive and in the staining of proteins in biomedical applications [23]. It is a purinergic P2X7 receptor antagonist.…”

Section: Rosmentioning

confidence: 99%

Novel Protective Effects of Brilliant Blue G in Acute Malathion Toxicity

Abdel-Salam

Sleem

Youness

et al. 2018

ROS

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We investigated the effect of brilliant blue G on the development of oxidative stress and tissue damage in rats with acute malathion intoxication. Rats received a single dose of malathion (150 mg/kg) intraperitoneally (ip) and then treated with either saline or brilliant blue G (5, 10, or 20 mg/kg, ip). The control group received saline. Rats were euthanized 4 h after the last injection, and the brain and liver were removed for biochemical studies including determination of the lipid peroxidation malondialdehyde (MDA), nitric oxide, reduced glutathione (GSH), paraoxonase-1 (PON-1), butyrylcholinesterase (BChE), and Na +-K + ATPase activities. Histopathological evaluation of the brain and liver tissue was also performed. Results indicated that rats treated with only malathion exhibited significantly increased MDA and nitric oxide in the brain and liver accompanied with significant decline in tissue levels of GSH. In addition, there was significant decline in brain and liver PON-1 activity and a significantly decreased brain BChE and Na +-K + ATPase activities. Brilliant blue G at doses of 5-20 mg/kg caused a dose-dependent decrease in MDA, and at doses of 10-20 mg/kg decreased nitric oxide, and increased GSH in the brain and liver of malathion-treated rats. It also increased PON-1 activity in the brain (at doses of 10-20 mg/kg) and liver (at a dose of 20 mg/kg). Brilliant blue G at doses of 5-20 mg/kg increased BChE activity and at doses of 10-20 mg/kg increased Na +-K +-ATPase activity in the brain of malathion-treated rats. In conclusion, treatment with brilliant blue G decreased oxidative stress in the brain and liver and restored BChE and Na +-K + ATPase activities in the brain of malathion-intoxicated rats. The dye afforded protection against malathion-induced neuronal and liver cell injury.

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“…1, E133) is a reddish-blue triarylmethane water-soluble dye used as a blue colorant. It has reasonable stability when exposed to light, heat and acidic conditions, but it has overall low oxidative stability [162]. This dye is considered harmless and most of the dye is excreted undigested [163].…”

Section: Diet Coloringmentioning

confidence: 99%

“…There is no evidence that this dye in rats or mice is carcinogenic, or neurotoxic [164]. However, this dye can act as purinergic inhibitor without pharmacological selectivity, thereby modulating some organ and tissue functions [162]. In addition, a recent report has demonstrated that this dye showed significant greater absorption in septic patients with reduced intestinal barrier function [165].…”

Section: Diet Coloringmentioning

confidence: 99%

“…In addition, a recent report has demonstrated that this dye showed significant greater absorption in septic patients with reduced intestinal barrier function [165]. The daily maximum FDA-approved uptake of Brilliant blue for humans is 12.5 mg/kg body weight/day, while the EU scientific committee suggested an acceptable daily intake (ADI) of 10 mg/kg body weight/day [162].…”

Section: Diet Coloringmentioning

confidence: 99%

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All You Can Feed: Some Comments on Production of Mouse Diets Used in Biomedical Research with Special Emphasis on Non-Alcoholic Fatty Liver Disease Research

et al. 2020

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The laboratory mouse is the most common used mammalian research model in biomedical research. Usually these animals are maintained in germ-free, gnotobiotic, or specific-pathogen-free facilities. In these facilities, skilled staff takes care of the animals and scientists usually don’t pay much attention about the formulation and quality of diets the animals receive during normal breeding and keeping. However, mice have specific nutritional requirements that must be met to guarantee their potential to grow, reproduce and to respond to pathogens or diverse environmental stress situations evoked by handling and experimental interventions. Nowadays, mouse diets for research purposes are commercially manufactured in an industrial process, in which the safety of food products is addressed through the analysis and control of all biological and chemical materials used for the different diet formulations. Similar to human food, mouse diets must be prepared under good sanitary conditions and truthfully labeled to provide information of all ingredients. This is mandatory to guarantee reproducibility of animal studies. In this review, we summarize some information on mice research diets and general aspects of mouse nutrition including nutrient requirements of mice, leading manufacturers of diets, origin of nutrient compounds, and processing of feedstuffs for mice including dietary coloring, autoclaving and irradiation. Furthermore, we provide some critical views on the potential pitfalls that might result from faulty comparisons of grain-based diets with purified diets in the research data production resulting from confounding nutritional factors.

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Brilliant Blue Dyes in Daily Food: How Could Purinergic System Be Affected?

Cited by 25 publications

References 93 publications

Selective inhibition of Panx1 channels decreases hemostasis and thrombosis in vivo

Selective inhibition of Panx1 channels decreases hemostasis and thrombosis in vivo

Novel Protective Effects of Brilliant Blue G in Acute Malathion Toxicity

All You Can Feed: Some Comments on Production of Mouse Diets Used in Biomedical Research with Special Emphasis on Non-Alcoholic Fatty Liver Disease Research

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