Aneurinibacillus tyrosinisolvens sp. nov., a tyrosine-dissolving bacterium isolated from organics- and methane-rich seafloor sediment

Abstract

A novel Gram-positive-staining, strictly aerobic and heterotrophic bacterium, designated strain LL-002 T , was isolated from organics-and methane-rich seafloor sediment at a depth of 100 m in Kagoshima Bay, Kagoshima, Japan. Colonies were lustreless and translucent white in colour. The temperature, pH and salt concentration ranges for growth were 10-30 8C, pH 6.0-6.5 and 0-1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain LL-002 T belongs to the genus Aneurinibacillus of the family Paenibacillaceae. 16S rRNA gene sequence similarities between strain LL-002 T and the type strains of species of the genus Aneurinibacillus were 92.8-95.7 %; the highest sequence identity was with the type strain of Aneurinibacillus migulanus. The DNA G+C content of strain LL-002 T was 46.2 mol%. MK-7was the predominant menaquinone. The predominant cellular fatty acids were iso-C 15 : 0 and anteiso-C 15 : 0 , and the cell-wall peptidoglycan contained meso-diaminopimelic acid and glutamic acid, glycine and alanine in addition to muramic acid and glucosamine. The peptidoglycan type was A1c. In DNA-DNA hybridization assays between strain LL-002 T and the type strains of the other species of the genus Aneurinibacillus, the level of hybridization was 6.3-30.1 %. On the basis of its biological features and the 16S rRNA gene sequence comparison presented here, strain LL-002 T is considered to represent a novel species of the genus Aneurinibacillus, for which the name Aneurinibacillus tyrosinisolvens sp. nov. is proposed; the type strain is LL-002 T (5NBRC 110097 T 5CECT 8536 T ).Bacillus aneurinolyticus was described as a thiaminedecomposing bacterium by Aoyama (1952) and the description of the species was revised by Shida et al. (1994). Aneurinibacillus and Brevibacillus were established as new genera arising from the reclassification of the Bacillus aneurinolyticus and Bacillus brevis groups, based on polyphasic taxonomic analyses (Shida et al., 1996). At the time of writing, six species have been described in the genus Aneurinibacillus with validly published names [Aneurinibacillus aneurinilyticus (Aoyama, 1952; Shida et al., 1994Shida et al., , 1996Heyndrickx et al., 1997), A. migulanus (Takagi et al., 1993;Shida et al., 1996;Heyndrickx et al., 1997), A. danicus (Goto et al., 2004), A. thermoaerophilus (Meier-Stauffer et al., 1996Heyndrickx et al., 1997), A. terranovensis (Allan et al., 2005) and A. soli (Lee et al., 2014] and 20 species have been assigned to the genus Brevibacillus. The genus Aneurinibacillus is closely related to the genus Brevibacillus, and it is difficult to distinguish species of the two genera by biochemical and chemotaxonomic assays and molecular analysis such as amplified rRNA gene restriction analysis (Logan et al., 2002). However, the sequence of the 59 hypervariable region of the 16S rRNA gene (nucleotide positions 70-344; Bacillus subtilis numbering) has proven to be a useful marker for

Cryptic fungal diversity revealed in deep-sea sediments associated with whale-fall chemosynthetic ecosystems

“…The aim of this study was to investigate fungal diversity in sediments associated with whale-fall chemosynthetic ecosystems in order to increase our knowledge of fungal communities in deep-sea environments, especially in unique chemosynthetic ecosystems. In this study, sediments of whale-fall chemosynthetic ecosystems from two different sites, one naturally occurring at 4200 m water depth in South Atlantic Ocean (Sumida et al 2016 ) and one artificially immersed at 100 m water depth in Kagoshima Bay, Japan (Fujiwara et al 2007 ; Tsubouchi et al 2015 ) were investigated by Ion Torrent PGM targeting ITS region of ribosomal RNA to reveal fungal community in these unique marine environments.…”

Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain

“…Bacteria were cultured on Tryptic soy agar (Merck, Germany) at 28 ± 1 °C for 24 h. Cellular fatty acids (FAs) were saponified, methylated and extracted according to the protocol of the Sherlock Microbial Identification system (version 6.21; MIDI) and FAs methyl esters profile was determined by gas chromatography by referencing the STBA6 Microbial Identification System (MIS) standard library [10].…”

Phyletic distribution and diversification of the Phage Shock Protein stress response system in bacteria and archaea

“…More than 60% of the 40,000 PSP proteins identified contained either PspA or PspC domains ( Figure 3A), including genomes containing multiple copies of the same PSP domain. For example, we identified eight PspA proteins in the genome of Aneurinibacillus tyrosinisolvens, which was recently isolated from methane-rich seafloor sediments (Tsubouchi et al 2015) (Table S1).…”