Anent the Genomics of Spermatogenesis in Drosophila melanogaster

Lindsley, Dan L.; Roote, John; Kennison, James A.

doi:10.1371/journal.pone.0055915

Cited by 33 publications

(36 citation statements)

References 26 publications

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“…Drosophila strains: For genetic nomenclature, cytology and description of mutations and chromosomes see [39] Phenotypic rescue experiments. The transgenic lines for UAS-DmManf and UAS-HsManf were recombined together with Actin-GAL4 driver, a GAL4 line of ubiquitous expression, on the DmManf Δ115 mutant background.…”

Section: Methodsmentioning

confidence: 99%

A Different Approach for Cellular Oncogene Identification Came from Drosophila Genetics

Magdalena¹,

Savu²

2013

Oncogene and Cancer - From Bench to Clinic

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Section: Methodsmentioning

confidence: 99%

A Different Approach for Cellular Oncogene Identification Came from Drosophila Genetics

Magdalena¹,

Savu²

2013

Oncogene and Cancer - From Bench to Clinic

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“…The flr 3 /TM3 strain contains the wing cell marker allele flare3 (flr 3 , 3 -38.8) and the balancer chromosomes TM. More detailed information on genetic markers and descriptions are given by Lindsley and Zimm [22].…”

Section: Mutagenicity Assaymentioning

confidence: 99%

Genotoxicity of Some Essential Oils Frequently Used in Aromatherapy

Mezzoug¹,

Idaomar²,

Baudoux³

et al. 2016

ABB

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Genotoxic properties of the essential oils extracted from Artemisia dracunculus (tarragon), Ocimum basilicum (basil), Cinnamomum loureirii (cinnamon), Laurus nobilis (laurel), Satureja montana (savory) and Rosmarinus officinallis (rosemary) are studied by Drosophila melanogaster Somatic Mutation and Recombination Test (SMART). The high bioactivation crossed with a high cytochrome P450-dependent bioactivation capacity is used. This assay is principally based on the loss of heterozygosity of the suitable recessive markers' multiple wing hairs (mwh) and flare-3 (flr 3 ) which can lead to the formation of mutant clones of larval cells, and which are then going to be expressed as spots on the wings of adult flies. Third-instar larvae are treated for 48 hr with different concentrations of the essential oils dissolved in Tween-80 at 0.2% or 2%. The wings of the emerging adults are analyzed for the occurrence of different types of mutant spots. No statistically significant differences in spot frequencies between negative controls and treated series are observed. These results suggest that the six essential oils at concentrations tested are not genotoxic towards somatic cells of D. melanogaster.

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“…For the description of the mutant alleles and GAL4 constructs see Lindsley and Zimm (1992) and FlyBase (www. flybase.org).…”

Section: Fly Culturementioning

confidence: 99%

An Unusual Role fordoublesexin Sex Determination in the DipteranSciara

et al. 2015

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The gene doublesex, which is placed at the bottom of the sex-determination gene cascade, plays the ultimate discriminatory role for sex determination in insects. In all insects where this gene has been characterized, the dsx premessenger RNA (premRNA) follows a sex-specific splicing pattern, producing male-and female-specific mRNAs encoding the male-DSXM and female-DSXF proteins, which determine male and female development, respectively. This article reports the isolation and characterization of the gene doublesex of dipteran Sciara insects. The Sciara doublesex gene is constitutively transcribed during development and adult life of males and females. Sciara had no sex-specific doublesex mRNAs but the same transcripts, produced by alternative splicing of its primary transcript, were present in both sexes, although their relative abundance is sex specific. However, only the female DSXF protein, but not the male DSXM protein, was produced at similar amounts in both sexes. An analysis of the expression of female and male Sciara DSX proteins in Drosophila showed that these proteins conserved female and male function, respectively, on the control of Drosophila yolk-protein genes. The molecular evolution of gene doublesex of all insects where this gene has been characterized revealed that Sciara doublesex displays a considerable degree of divergence in its molecular organization and its splicing pattern with respect to the rest of dipterans as suggested by its basal position within the doublesex phylogeny. It is suggested that the doublesex gene is involved in Sciara sex determination although it appears not to play the discriminatory role performed in other insects.KEYWORDS sex determination; molecular evolution; Sciara; gene doublesex; genetics of sex M ALES and females are different at the morphological, physiological, and behavioral levels. This sexual dimorphism results from the integration of two processes: sex determination and sexual differentiation. Sex determination refers to the developmental program that commits the embryo to either the male or the female pathway. The genes underlying this phenomenon are the sex-determination genes. Sexual differentiation refers to the expression of the sex cytodifferentiation genes (which are controlled by the sex-determination genes), the expression of which gives rise to the formation of the sexually dimorphic structures that characterize the male and female adults.The animal kingdom possesses a wealth of mechanisms by which sex is decided (Bull 1983;Beukeboom and Perrin 2014). These mechanisms can be classified into three main categories, depending on the origin of the primary sexdetermination signal, which can be zygotic, maternal, or environmental. These three primary signals occur and are concatenated in the dipteran Sciara (for details see Sánchez 2010). Briefly, in Sciara, sex determination follows the XX/X0 mechanism: XX zygotes will develop as females and X0 zygotes will develop as males. Yet, the final chromosome constitution of the zygote, which start...

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Anent the Genomics of Spermatogenesis in Drosophila melanogaster

Cited by 33 publications

References 26 publications

A Different Approach for Cellular Oncogene Identification Came from Drosophila Genetics

A Different Approach for Cellular Oncogene Identification Came from Drosophila Genetics

Genotoxicity of Some Essential Oils Frequently Used in Aromatherapy

An Unusual Role fordoublesexin Sex Determination in the DipteranSciara

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