Anemia Severity in β-Thalassemia Correlates with Elevated Levels of microRNA-125b in Activated Phagocytic Monocytes

Kuno, Suhaibee; Penglong, Tipparat; Srinoun, Kanitta

doi:10.1080/03630269.2019.1628043

Cited by 8 publications

(8 citation statements)

References 30 publications

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“…the higher miR-125b expression in CD14-positive monocytic cells of β-thalassemia patients was positively correlated to phagocytic activity while negatively correlated to anemia severity (Kuno et al, 2019). In this study, both miR-155 and miR-125b were higher upregulated after treatment with the lower concentration of PMA and that correlated with the increasing of phagocytic activity of macrophage.…”

Section: Discussionsupporting

confidence: 56%

“…Another study found that miR-125b was enriched in macrophages and partly responsible for macrophage activation, at least partially by reducing IFN regulatory factor 4 (IRF4) levels (Chaudhuri et al, 2011). Our previous study found the higher miR-125b expression in CD14-positive monocytic cells of β-thalassemia patients and that correlated with the higher phagocytic activity (Kuno et al, 2019). IRF4 is a member of the IFN response factor family of transcription factors, which has a function to inhibit the inflammatory response (Honma et al, 2005;Negishi et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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The effects of Phorbol 12-myristate 13-acetate concentration on the expression of miR-155 and miR-125b and their macrophage function-related genes in the U937 cell line

2020

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The phorbol 12-myristate 13-acetate (PMA)-induced U937 cell line has been widely used as an in vitro model for studying the functions of human macrophages. However, there are several concentrations of PMA commonly used to drive the differentiation of monocytic cell line to macrophage. Also, the expression of microRNA-155 (miR-155) and miR-125b in PMA-treated human monocytic cell line has not yet been reported. The five usual concentrations of PMA for stimulating macrophage differentiation are 10, 25, 50, 100, and 200 nM. In this study we compared the expression levels of miR-155, miR-125b and their related genes involved in macrophage functions in U937-derived cells after treatment with those five concentrations. The morphological study results showed that the five concentrations of PMA could induce macrophage differentiation in a similar manner. Moreover, cell proliferation and viability were not significantly different among these five conditions excepted the lower cell viability at 200 nM of PMA treatment. The five concentrations of PMA could upregulate the expression of miR-155 and miR-125b and increase the phagocytic activity of U937-derived cells in dose-reversal manner. The upregulation of miR-155 was correlated with increased expression levels of TNFα and decreased expression levels of BACH1 and CEBPβ, while the reduction of IRF4 was correlated with increased expression levels of miR-125b. Our study found that PMA could stimulate macrophage differentiation in a broad range of concentrations, however, the lower concentration could upregulate the higher expression of both miR-155 and miR-125b, and that correlated with the phagocytic functional activity of U937-derived macrophages.

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Section: Discussionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

The effects of Phorbol 12-myristate 13-acetate concentration on the expression of miR-155 and miR-125b and their macrophage function-related genes in the U937 cell line

2020

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“…Additionally, one of the serious complications of thalassemia is thalassemia bone disease ( Wang et al., 2021 ). Therefore, increasing the expression levels of fetal hemoglobin (HbF) or regulating the expression of α-, β- and γ-globin are considered promising and potential treatments against thalassemia bone disease ( Eltaweel et al., 2021 ; Kuno et al., 2019 ). Importantly, in thalassemia bone disease eight miRNAs namely hsa-miR-146b-5p, hsa-miR-146a-5p hsa-miR-148b-3p, hsa-miR-155-5p, hsa-miR-192-5p, hsa-miR-335-5p, hsa-miR-7-5p, hsa-miR-98-5p were found to be upregulated followed by the downregulation of hsa-miR-320a, hsa-miR-92a-3p, hsa-let-7a-5p and hsa-miR-92a-3p ( Das et al., 2021 ).…”

Section: Therapeutic Role Of Mirna In Several Bone Diseasesmentioning

confidence: 99%

“…miR-125b is expressed in higher amounts in monocytic cells of β-thalassemia patients and correlates negatively with abnormal red blood cells (RBCs) and hemoglobin levels. Thus, miR-125b might act as a potential therapeutic agent against thalassemia ( Kuno et al., 2019 ). miR-30a also ameliorates thalassemia by regulating HbF expression by targeting the BCL11A gene ( Gholampour et al., 2020 ).…”

Section: Therapeutic Role Of Mirna In Several Bone Diseasesmentioning

confidence: 99%

Recent advancements of miRNAs in the treatment of bone diseases and their delivery potential

Sharma

Lee

2023

Current Research in Pharmacology and Drug Discovery

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“…Therefore, these miRNAs are considered as potential theragnostic targets for thalassemia [ 157 ]. Kuno et al (2019) [ 158 ] noticed a significant upregulation of miR-125b in activated phagocytic monocytes of patients with β-thalassemia. These expression levels were linked with the phagocytic activity of the monocytes; in addition, miR-125b may possess a noteworthy role as a genetic modifier in anemia severity in patients with β-thalassemia.…”

Section: Other Bone Diseases and Mirnasmentioning

confidence: 99%

The Emerging Role of MicroRNAs in Bone Diseases and Their Therapeutic Potential

et al. 2021

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MicroRNAs (miRNAs) are a class of small (20–24 nucleotides), highly conserved, non-coding RNA molecules whose main function is the post-transcriptional regulation of gene expression through sequence-specific manners, such as mRNA degradation or translational repression. Since these key regulatory molecules are implicated in several biological processes, their altered expression affects the preservation of cellular homeostasis and leads to the development of a wide range of pathologies. Over the last few years, relevant investigations have elucidated that miRNAs participate in different stages of bone growth and development. Moreover, the abnormal expression of these RNA molecules in bone cells and tissues has been significantly associated with the progression of numerous bone diseases, including osteoporosis, osteosarcoma, osteonecrosis and bone metastasis, among others. In fact, miRNAs regulate multiple pathological mechanisms, including altering either osteogenic or osteoblast differentiation, metastasis, osteosarcoma cell proliferation, and bone loss. Therefore, in this present review, aiming to impulse the research arena of the biological implications of miRNA transcriptome in bone diseases and to explore their potentiality as a theragnostic target, we summarize the recent findings associated with the clinical significance of miRNAs in these ailments.

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Anemia Severity in β-Thalassemia Correlates with Elevated Levels of microRNA-125b in Activated Phagocytic Monocytes

Cited by 8 publications

References 30 publications

The effects of Phorbol 12-myristate 13-acetate concentration on the expression of miR-155 and miR-125b and their macrophage function-related genes in the U937 cell line

The effects of Phorbol 12-myristate 13-acetate concentration on the expression of miR-155 and miR-125b and their macrophage function-related genes in the U937 cell line

Recent advancements of miRNAs in the treatment of bone diseases and their delivery potential

The Emerging Role of MicroRNAs in Bone Diseases and Their Therapeutic Potential

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