Androgens regulate expression of the gene coding for a mouse vas deferens protein related to the aldo-keto reductase superfamily in epithelial cell subcultures

Dassouli, Amina; Manin, M.; Veyssière, G.; Jean, C

doi:10.1016/0960-0760(94)90258-5

Cited by 25 publications

(15 citation statements)

References 34 publications

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“…Activation of the MVDP gene by androgens in vas deferens epithelial cells is sensitive to cycloheximide, indicating a need for de novo protein synthesis (Dassouli et al 1994). Thus the stimulation of the MVDP gene is regulated by different pathways depending on cell type.…”

Section: Figurementioning

confidence: 93%

Cyclic AMP regulates expression of the gene coding for a mouse vas deferens protein related to the aldo-keto reductase superfamily in human and murine adrenocortical cells

Aigueperse

Martinez²,

Lefrançois‐Martinez³

et al. 1999

Journal of Endocrinology

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Mouse vas deferens protein (MVDP) is a member of the aldo-keto reductase superfamily. The regulation of MVDP gene expression by activators of the protein kinase A signalling pathway was investigated in human (H295-R) and murine (Y1) adrenocortical carcinoma cells. Immunoblotting with polyclonal antibodies showed that MVDP is expressed in adrenal glands from mouse, rat, rabbit and guinea-pig, probably under the control of ACTH. In both adrenocortical cell lines used, MVDP is constitutively synthesized and its accumulation is increased by treatment with cAMP or forskolin. MVDP mRNA steady-state levels were up-regulated by forskolin in adrenocortical cells by a process that does not require de novo protein synthesis. The results suggest that cAMP is at least one of the key regulators of adrenal MVDP expression and that this effect is direct.

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Section: Figurementioning

confidence: 93%

Cyclic AMP regulates expression of the gene coding for a mouse vas deferens protein related to the aldo-keto reductase superfamily in human and murine adrenocortical cells

Aigueperse

Martinez²,

Lefrançois‐Martinez³

et al. 1999

Journal of Endocrinology

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“…Androgendependent specific protein synthesis has been demonstrated in mouse vas deferens epithelial cells in culture [8]. There was a close relationship between the specific protein m R N A levels and dihydrotestosterone concentrations, suggestive of D H T -m e d i a t e d protein synthesis in target tissue.…”

Section: Discussionmentioning

confidence: 93%

“…The anabolic effects of the androgen testosterone and its matrix stimulatory metabolite DHT on bone and connective tissue have been well documented [7][8][9][10]. The conversion of testosterone to DHT is enhanced in cells from an inflamed source [11,12] and in the gingival overgrowth phenomenon [13].…”

Section: Introductionmentioning

confidence: 99%

The combined effects of TGF-β, IGF and PDGF on 5α-reductase activity on androgen substrates in human gingival tissue

Kasasa

Soory

1998

Inflammopharmacol

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The combined effects of the growth factors, PDGF, TGF-beta and IGF, on the metabolism of two androgen substrates by human gingival tissue were investigated. Having established their wet weight, duplicate incubations were performed in Eagle's MEM using [(14)C]testosterone/[(14)C]4-androstenedione as substrates and growth factors, PDGF, TGF-beta and IGF, alone and in combination. Steroid metabolites were then isolated, separated and quantified, using a radioisotope scanner. With [(14)C]testosterone as substrate, there were 3-5-fold decreases in 5alpha-reductase activity in response to individual growth factors, while the combinations, PDGF+TGF-beta, PDGF+IGF and TGF-beta-IGF, resulted in approximately half the stimulation, or similar to that of one of the growth factors, but still about 2-fold greater than control values. When [(14)C]4-androstenedione was used as substrate, there were approximately 2-6-fold increases in DHT synthesis in response to the growth factors alone. When used in combination, and intermediate response was seen. Growth factor combinations can enhance anabolic activity in the chronically inflamed periodontium.

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“…The physiologically active androgens testosterone and DHT are known to exert anabolic effects on connective tissues and bone in mammals (Dassouli et al. 1994, Colvard et al.…”

mentioning

confidence: 99%

Phenytoin‐mediated androgen metabolism in gingival fibroblasts

Soory¹,

Suchak²

2002

J Clinic Periodontology

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Androgens regulate expression of the gene coding for a mouse vas deferens protein related to the aldo-keto reductase superfamily in epithelial cell subcultures

Cited by 25 publications

References 34 publications

Cyclic AMP regulates expression of the gene coding for a mouse vas deferens protein related to the aldo-keto reductase superfamily in human and murine adrenocortical cells

Cyclic AMP regulates expression of the gene coding for a mouse vas deferens protein related to the aldo-keto reductase superfamily in human and murine adrenocortical cells

The combined effects of TGF-β, IGF and PDGF on 5α-reductase activity on androgen substrates in human gingival tissue

Phenytoin‐mediated androgen metabolism in gingival fibroblasts

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