Androgenic studies in the production of haploids and doubled haploids in Capsicum spp.

Sánchez, Manuel Alejandro; Coronado, Ana Cruz Morillo

doi:10.15446/rfnam.v73n1.76044

Cited by 9 publications

(8 citation statements)

References 56 publications

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“…Capsicum spp. is a horticulture crop with great potential and is supposed to be the fourth most significant vegetable in the earth (Sanchez et al, 2020). It is a leading vegetable crop in our country.…”

Section: Introductionmentioning

confidence: 99%

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Jha¹,

Choudhary²,

Agarwal³

2021

PLANT ARCHIVES

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Capsicum spp. is a horticulture crop with great potential and is supposed to be the fourth most significant vegetable in the earth. Capsicum genus covers several dozens of species which contain vast range of nutritional and medicinal properties. The process of inbreed development for hybrid variety production is fastened by the use of doubled haploid (DH) system. Doubled haploid (DH) system plays an important role in breeding programs and developmental studies. The production of haploid and doubled haploid plants using the anther cultures of the desired genotype is one of opportunities for rapid stabilization of new variants. There is an ongoing research to develop and improve the effectiveness of these methods by looking at the factors involved in different phases like induction and regeneration. Factors predominantly incorporate condition of donor plants, plant genotypes, medium components, cultured gametophytic cells temperature treatments of (cold pre-treatment, heat shock), the developmental period of early on gametophytic cells and use of various segments like Charcoal and plant development regulators. Stress treatment is one of the conditions which are utilized for the incitement of gametophytic cells to modify toward the sporophytic pathway.

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Section: Introductionmentioning

confidence: 99%

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Jha¹,

Choudhary²,

Agarwal³

2021

PLANT ARCHIVES

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“…Although recent advances in in vitro techniques have demonstrated their role in shortening the breeding cycles unequivocally (Barroso et al 2019;Niazian and Shariatpanahi 2020); however, these techniques are still largely underutilized in most pepper breeding programs, which are still reliant on conventional breeding (Sánchez et al 2020). This is particularly prevalent in Bulgaria and across the rest of the Balkans (Nankar et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Creation of pepper doubled haploids and morphological characterization of androgenic plants

2021

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The Balkan pepper breeding program aims to use in vitro embryogenesis (anther culture) to develop doubled haploid pepper lines with valuable traits. We analyzed the effectiveness of microspore embryogenesis on 17 pepper genotypes comprised of varieties, breeding lines, and F1 hybrids of different varietal types (conical, bell shape, and kapia) and characterized the agronomic traits of newly generated doubled haploid lines. The highest androgenic potential was observed in the variety Stryama and breeding line 560/06 with 9.55 and 13.00% reacted anthers, respectively. Of the 186 regenerated plants, 147 were successfully adapted with a survival index of 79.03%. Flow cytometry analysis showed that the haploid:diploid ratio of regenerants was 1.5:1. All diploid plants were confirmed to be androgenic in origin. There were significant differences in terms of quantitative fruit characteristics of length, width, weight, pericarp weight, pericarp thickness, and productivity per plant among the diploid lines. Also, androgenic lines 21, 23, and 74 derived from Stryama and line 55 obtained from Zlaten medal 7 were distinguished with higher values of some fruit traits compared to initial genotypes. Additionally, several androgenesis lines (Stryama lines 21, 23, and 74, and Zlaten medal 7 line 55) scored higher for some fruit traits compared to the initial genotype. These results show that anther culture is a promising tool for the creation of Balkan pepper breeding lines with improved traits.

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“…The stress treatments to anthers cultures are an essential factor in androgenic response. It has been shown that stress pre-treatments applied to anthers and microspores, inducing the sporophytic pathway, inhibit the gametophytic pathway (fertile pollen development), and increase embryogenic potential (Sanchez et al 2020). Several pre-treatments protocols have been reported to induce microspore embryogenesis and plant regeneration in different varieties.…”

Section: Introductionmentioning

confidence: 99%

“…Several pre-treatments protocols have been reported to induce microspore embryogenesis and plant regeneration in different varieties. Pretreatments of anther culture result in an increased androgenic response (Irikova et al, 2011, Perez-Perez 2019, Sanchez et al 2020. Although stress pre-treatments are necessary to initiate the sporophytic development of microspores, these pre-treatments could also cause the cells to lose their viability.…”

Section: Introductionmentioning

confidence: 99%

Effects of Biotin and Ascorbic Acid Applications on Haploid Embryo Induction in Semisolid and Double Layer Nutrient Media in Pepper (Capsicum annuum L.) Anther Culture

Demirkaya

Çömlekçioğlu

2021

International Journal of Agriculture Environment and Food Sciences

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Generation of homozygous double haploid (DH) lines by androgenesis is a promising alternative to selfpollination programs across generations. Despite the routine use of anther culture in peppers, there are still many bottlenecks and improvements in methodology are required. The aim of this study was to determine the effects of the structure of the nutrient medium (semi-solid and double layer) and the addition of biotin and ascorbic acid to the nutrient media on obtaining haploid embryos by anther culture method. MS (Murashige and Skoog 1962) medium containing 4 mg l -1 NAA, 0.1 mg l -1 BAP, 0.25% activated charcoal, 30 g l -1 sucrose, and 10 mg l -1 AgNO3 (silver nitrate) were used as the basal nutrient medium. A total of 8 nutrient media compounds were studied using 0.05 mg l-1 biotin and 0.5 mg l -1 ascorbic acid separately or together in semi-solid and bi-layer (double-phase) nutrient media. Solidification of nutrient media was achieved with 7 g 1 -1 agar. The cultured anthers were subjected to high-temperature pre-treatments at 35 °C in continuous dark conditions for 2 days. Then they were taken to a climate chamber at of 25 °C temperature adjusted to 16/8 hour photoperiod. It has been observed that the success of obtaining embryos of semi-solid medium was higher than double-layer medium. The addition of biotin and ascorbic acid to the nutrient medium provided 8.8 fold increases in embryo regeneration compared to the control medium. In the presence of only one of biotin or ascorbic acid in the nutrient medium, the number of embryos increased compared to the control.

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Androgenic studies in the production of haploids and doubled haploids in Capsicum spp.

Cited by 9 publications

References 56 publications

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Creation of pepper doubled haploids and morphological characterization of androgenic plants

Effects of Biotin and Ascorbic Acid Applications on Haploid Embryo Induction in Semisolid and Double Layer Nutrient Media in Pepper (Capsicum annuum L.) Anther Culture

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