Androgen glucuronides, instead of testosterone, as the new markers of androgenic activity in women

Labrie, Fernand; Bélanger, Alain; Bélanger, Patrick; Bérubé, René; Martel, Céline; Cusan, Lionel; Gómez, José L.; Candas, Bernard; Castiel, Isabelle; Chaussade, V.; Deloche, Claire; Leclaire, Jacques

doi:10.1016/j.jsbmb.2006.02.004

Cited by 270 publications

(251 citation statements)

References 23 publications

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“…Mice under isoflurane anesthesia were exsanguinated by cardiac venipuncture. Blood samples were processed for serum preparation and kept frozen at Ϫ80°C until use for the determination of serum DHT concentrations by gas chromatography mass spectrometry as previously described (23) with the exception of the serum volume used (0.15-0.3 ml in mice instead of 0.75 ml in humans). Serum samples from two mice of the same group were pooled for each determination of DHT levels (n ϭ 6 for DHT groups; n ϭ 12 for the control group).…”

Section: Methodsmentioning

confidence: 99%

Response of the adipose tissue transcriptome to dihydrotestosterone in mice

Zhang

Calvo

Martel

et al. 2008

Physiological Genomics

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Section: Methodsmentioning

confidence: 99%

Response of the adipose tissue transcriptome to dihydrotestosterone in mice

Zhang

Calvo

Martel

et al. 2008

Physiological Genomics

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“…LH, FSH, and SHBG were measured as described previously (3,7). T and E 2 were measured using gas chromatography-mass spectrometry as described before (11,12). The lower limit of quantification of T was 170 pmol/liter, and of E 2 , 2.2 pmol /liter.…”

Section: Hormone Assaysmentioning

confidence: 99%

Effect of Polymorphisms in Selected Genes Involved in Pituitary-Testicular Function on Reproductive Hormones and Phenotype in Aging Men

Huhtaniemi

Pye

Holliday

et al. 2010

The Journal of Clinical Endocrinology & Metabolism

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, and the European Male Aging Study Group* † Context: Polymorphisms in genes involved in regulation, biosynthesis, metabolism, and actions of testicular sex hormones may influence hormone balance and phenotype of aging men. Objective:We investigated the relationships between polymorphisms in genes related to pituitarytesticular endocrine function and health status. Design and Setting:Using cross-sectional baseline data, we conducted a multinational prospective cohort observational study consisting of a population survey of community-dwelling men.Participants: A total of 2748 men, aged 40 -79 (mean Ϯ SD, 60.2 ϩ 11.2) yr, were randomly recruited from eight European centers. Forty-three polymorphisms were genotyped in the following genes: androgen receptor (AR), estrogen receptor-␣ and -␤ (ESR1 and ESR2), steroid 5␣-reductase type II (SRD5A2), 17␣-hydroxylase/17,20-lyase (CYP17A1), aromatase (CYP19A1), sex hormone-binding globulin (SHBG), LH ␤-subunit (LHB), and LH receptor (LHCGR). Main Outcome Measures:We measured the associations between gene polymorphisms and endocrine, metabolic, and phenotypic parameters related to aging and sex hormone action. Results: Several polymorphisms in SHBG, ESR2, AR, CYP19A1, and LHB were significantly associated with circulating levels of SHBG, LH, total, free, and bioavailable testosterone and estradiol, the LH ϫ testosterone product, and indices of insulin sensitivity. Apart from several previously reported associations between genes affecting estrogen levels and heel ultrasound parameters, no associations existed between polymorphisms and nonhormonal variables (anthropometry, blood lipids, blood pressure, hemoglobin, prostate symptoms, prostate-specific antigen, sexual dysfunction, cognition). Conclusion:In aging men, polymorphisms in genes related to the pituitary-testicular endocrine function significantly influence circulating LH, testosterone, and estradiol levels, but the downstream effects may be too small to influence secondary phenotypic parameters. (J Clin Endocrinol Metab 95:

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“…Serum levels of testosterone and E 2 were analyzed at the Laboratory of Molecular Endocrinology and Oncology, Laval University Hospital Research Center, Québec, Canada, as previously described (23,24). Briefly, the validated GC-MS system was used for the analyses of testosterone (limit of detection 0.05 ng/ml, intra-assay CV 2.9%, inter-assay CV 3.4%) …”

Section: Assessment Of Sex Hormones In Serummentioning

confidence: 99%

Serum estradiol is associated with lean mass in elderly Swedish men

Vandenput

Mellström

Karlsson

et al. 2010

European Journal of Endocrinology

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Objective: Association studies in men have shown that androgens are inversely related to fat measures, while the relation between sex steroids and lean mass remains unclear. We, therefore, investigated the associations between serum sex steroid levels and body composition in elderly men with a main focus on lean mass measures. Design and methods: A cross-sectional survey of a population-based cohort of 3014 elderly men, aged 69-80 years (Osteoporotic Fractures in Men study, Sweden). Serum levels of testosterone and estradiol (E 2 ) were measured by mass spectrometry, sex hormone-binding globulin (SHBG) levels were measured by IRMA, and measures of body composition were obtained by dual-energy X-ray absorptiometry. Results: Total as well as free serum testosterone associated independently inversely (P!0.001), while total as well as free serum E 2 associated independently directly (P!0.001) with total body fat mass and trunk fat mass. Serum SHBG associated independently inversely with central fat distribution. Serum E 2 and free E 2 but not serum testosterone or free testosterone levels associated positively with lean mass (P!0.01). Elderly men within the lowest quartile of free E 2 had 0.5 kg less lean mass in the legs than subjects within the highest quartile, while the subjects in the different quartiles of free testosterone did not differ in lean mass. Conclusions: Serum E 2 , but not serum testosterone, is directly associated with lean mass in this large study of elderly Swedish men. In addition, serum SHBG is associated with central fat distribution and we confirmed that serum testosterone is inversely associated with fat mass.

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Androgen glucuronides, instead of testosterone, as the new markers of androgenic activity in women

Cited by 270 publications

References 23 publications

Response of the adipose tissue transcriptome to dihydrotestosterone in mice

Response of the adipose tissue transcriptome to dihydrotestosterone in mice

Effect of Polymorphisms in Selected Genes Involved in Pituitary-Testicular Function on Reproductive Hormones and Phenotype in Aging Men

Serum estradiol is associated with lean mass in elderly Swedish men

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