Response of the adipose tissue transcriptome to dihydrotestosterone in mice

Zhang, Yonghua; Calvo, Ézéquiel; Martel, Céline; Luu‐The, Van; Labrie, Fernand; Tchernof, André

doi:10.1152/physiolgenomics.00257.2007

Cited by 17 publications

(19 citation statements)

References 48 publications

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“…Also adult, 90-day-old rats from the F1:Fin group were heavier then control rats. These observations can be associated with the modulation of adipose tissue metabolism by androgens [58], and therefore could be affected by changes in T/DHT ratio observed in our experiment. Interestingly, also testis weight of the 14, 22, and 90-day-old F1:Fin rats were statistically heavier than that of the similarly aged rats in the F1:Control group, proportionally to the higher body mass of these animals.…”

Section: Discussionsupporting

confidence: 52%

Androgen levels and apoptosis in the testis during postnatal development of finasteride-treated male rat offspring

Kolasa

Misiakiewicz-Has

Baranowska-Bosiacka

et al. 2015

Folia Histochem Cytobiol.

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Introduction. The hormone-dependent events that occur throughout the first wave of spermatogenesis, such as the establishment of the number of Sertoli cells (SCs) and spermatogonial stem cells (SSCs) within the seminiferous cords and the setting up of the blood-testis barrier, are important for adult male fertility. Any changes in the T/DHT ratio can result in male subfertility or even infertility. In this study we aimed to evaluate effects of paternal exposure to 5-alpha reductase type 2 inhibitor, finasteride on litter size, androgen levels and germ cell apoptosis in male offspring during postnatal development. Material and methods. The subjects of the study were 7, 14, 21/22, 28, and 90-day-old Wistar male rats (F1:Fin) born from females fertilized by finasteride-treated rats. Offspring born from untreated parental animals were used as a control group (F1:Control). Animals and the collected testes were weighed, blood and intratesticular levels of T and DHT were measured by ELISA, and the apoptotic index of testicular cells was evaluated by TUNEL technique. Results. We observed difficulties in obtaining male newborns from female rats fertilized by finasteride-treated male rats. In the F1:Fin rats, changes in the body and testes weights occurred, and a lower number of apoptotic cells was found during postnatal maturation of the seminiferous epithelium. Changes in androgen concentrations during the first spermatogenesis wave and adult life were also evident. Conclusion. Finasteride treatment of male adult rats may not only cause a decrease in the fertility of parental rats, but also could lead to incorrect, androgen-sensitive course of spermatogenesis in their offspring.

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Section: Discussionsupporting

confidence: 52%

Androgen levels and apoptosis in the testis during postnatal development of finasteride-treated male rat offspring

Kolasa

Misiakiewicz-Has

Baranowska-Bosiacka

et al. 2015

Folia Histochem Cytobiol.

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“…However, further specifications are needed in the case of female mice, in which the DHT dose considered may result in supraphysiological circulating levels, at least in the first 6 hours after the injection. In fact, as shown by Zhang et al [18], at 3 h, DHT serum concentration should reach 5-6 ng/ml, and then gradually decrease to more physiological levels [19,20], being finally lower than 500 pg/ml (18-24 h). Immediately following the sacrifice, the retroperitoneal fat was dissected (between 09:00 and 12:15 h).…”

Section: Sample Preparationmentioning

confidence: 92%

“…First strand cDNA was synthesized using 5 g of pooled RNA of each experimental group in a reaction containing 200 U of Superscript III Rnase H-RT (Invitrogen Canada Inc.), 300 ng of oligo-dT 18 , 500 M deoxynucleotides triphosphate, 10 mM dithiothreitol and 34 U of human RNase inhibitor (Amersham Pharmacia, Piscataway, NJ) in a final volume of 50 l. The resulting products were then treated with 1 g of Rnase A for 30 min at 37 • C and purified thereafter with Qiaquick PCR purification kits (Qiagen). The cDNA corresponding to 20 ng of total RNA was used to perform fluorescent-based real-time PCR quantification using the LightCycler real-time PCR apparatus (Roche Inc., Nutley, NJ) and the FastStart DNA Master SYBR green kit (Roche Diagnostics).…”

Section: Validation By Quantitative Real-time Pcr (Q Rt-pcr)mentioning

confidence: 99%

A single dose of dihydrotestosterone induced a myogenic transcriptional program in female intra-abdominal adipose tissue

Giorgio¹,

Yoshioka²,

St‐Amand³

2010

The Journal of Steroid Biochemistry and Molecular Biology

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“…In this study, the weights of the epididymides from the F1:Fin rats were higher than the control animals of the same age (apart from PND 28 -similar testis weight [38]), probably because of a correlation with total body weight or the same weight fluctuation differences in the testes [38]. These observations may be associated with the modulation of adipose tissue metabolism by androgens [61], and therefore could be affected by changes in the T/DHT ratio observed in our experiment.…”

Section: Weight Of Epididymidesmentioning

confidence: 44%

Antioxidant enzyme expression of mRNA and protein in the epididymis of finasteride-treated male rat offspring during postnatal development

Kolasa¹,

Tarnowski²,

Baranowska-Bosiacka³

et al. 2019

aoms

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A b s t r a c tIntroduction: We verify whether finasteride had a transgenerational effect on the epididymal expression of antioxidant enzymes, and the correlation between these enzymes and blood androgen concentrations in male offspring (F1:Fin) of females fertilized by finasteride-treated male rats. Material and methods: The expression of CAT, SOD1, GPX5, GR on the mRNA and protein levels was evaluated in the epididymis at postnatal day (PND) 7, 14, 21, 28 and 90. Levels of T and DHT were correlated with mRNA levels of enzymes by Spearman's rank correlation coefficient. Results: A change in the levels of transcripts was noted in F1:Fin rats: CAT decreased at PND 28 (p < 0.01) and increased at PND 90 (p < 0.01); SOD1 increased at PND 7 (p < 0.0001), 21 (p < 0.001), 90 (p < 0.0001) and decreased at 14 PND (p < 0.01); GPX5 increased at PND 14 and 21 (p < 0.0001); GR decreased at PND 21 and 28 (p < 0.0001). Altered immunolocalization of enzymes within the epididymal epithelium was observed. Negative correlations between GPX5 mRNA with androgens (T, p = 0.0002; DHT, p = 0.0009) were visible in the control rats, and positive correlation between DHT and CAT mRNA (p = 0.03), in opposite to F1:Fin group were was negative for both androgens (T, p = 0.044 and DHT, p = 0.02). Conclusions: Finasteride treatment of adult male rats may cause changes in antioxidant defense system in the epididymis of their offspring, leading to improper ROS concentrations that can affect post-testicular sperm maturation.

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Response of the adipose tissue transcriptome to dihydrotestosterone in mice

Abstract: Zhang Y, Calvo E, Martel C, Luu-The V, Labrie F, Tchernof A. Response of the adipose tissue transcriptome to dihydrotestosterone in mice.

Cited by 17 publications

References 48 publications

Androgen levels and apoptosis in the testis during postnatal development of finasteride-treated male rat offspring

Androgen levels and apoptosis in the testis during postnatal development of finasteride-treated male rat offspring

A single dose of dihydrotestosterone induced a myogenic transcriptional program in female intra-abdominal adipose tissue

Antioxidant enzyme expression of mRNA and protein in the epididymis of finasteride-treated male rat offspring during postnatal development

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