AND-gate contrast agents for enhanced fluorescence-guided surgery

Widen, John C.; Tholen, Martina; Yim, Joshua J.; Antaris, Alexander L.; Casey, Kerriann M.; Rogalla, Stephan; Klaassen, Alwin; Sorger, Jonathan M.; Bogyo, Matthew

doi:10.1038/s41551-020-00616-6

Cited by 99 publications

(106 citation statements)

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“…[ 57 ] Furthermore, protease activity has been integrated in synthetic logic circuits, which could further enhance detection specificity of synthetic biomarkers. [ 154–157 ] Overall, the results discussed in this section have demonstrated the potential of synthetic biomarkers for assessing anti‐tumor T cell responses during engineered T cell therapy.…”

Section: Identifying Predictive Biomarkers Of T Cell Responsementioning

confidence: 88%

Interfacing Biomaterials with Synthetic T Cell Immunity

Mac

Sivakumar

et al. 2021

Adv Healthcare Materials

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The clinical success of cancer immunotherapy is providing exciting opportunities for the development of new methods to detect and treat cancer more effectively. A new generation of biomaterials is being developed to interface with molecular and cellular features of immunity and ultimately shape or control anti‐tumor responses. Recent advances that are supporting the advancement of engineered T cells are focused here. This class of cancer therapy has the potential to cure disease in subsets of patients, yet there remain challenges such as the need to improve response rates and safety while lowering costs to expand their use. To provide a focused overview, recent strategies in three areas of biomaterials research are highlighted: low‐cost cell manufacturing to broaden patient access, noninvasive diagnostics for predictive monitoring of immune responses, and strategies for in vivo control that enhance anti‐tumor immunity. These research efforts shed light on some of the challenges associated with T cell immunotherapy and how engineered biomaterials that interface with synthetic immunity are gaining traction to solve these challenges.

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Section: Identifying Predictive Biomarkers Of T Cell Responsementioning

confidence: 88%

Interfacing Biomaterials with Synthetic T Cell Immunity

Mac

Sivakumar

et al. 2021

Adv Healthcare Materials

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“…Recently, Bogyo and co‐workers reported the AND‐gate FLI probes. [ 83 ] The fluorophore was designed in the middle of probe and was functionalized with two responsive peptides that linked quenchers to silence fluorescence emission, which could be cleaved by multiple specific enzymes to activate fluorescence signals in targeted site. Peptides, Lys‐Phe and Lys‐Asp‐Glu‐Val‐Asp‐Gly‐Pro, were chosen to be specifically and orthogonally cleaved by caspase 3 and cysteine cathepsins, which are overexpressed in tumor cells.…”

Section: Peptide‐ and Polypeptide‐based Materials For Biomedical Imagingmentioning

confidence: 99%

Advances and Perspectives of Peptide and Polypeptide‐Based Materials for Biomedical Imaging

Cornel

Fan

et al. 2021

Advanced NanoBiomed Research

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Biomedical imaging is an effective tool to reveal internal structures beneath the skin. Such tools are of great importance for disease diagnosis and treatment. Various small molecules, polymers, and micro‐ or nanomaterials have been applied as imaging agents for contrast enhancement. Peptide‐ and polypeptide‐based materials have been designed and developed for enhanced and multifunctional imaging, due to their excellent biocompatibility and diverse biofunctionality. This review aims to summarize recent advances in peptide‐ and polypeptide‐based materials for X‐ray computed tomography (CT), magnetic resonance imaging (MRI), fluorescence imaging (FLI), positron emission tomography (PET), single‐photon emission computed tomography (SPECT), and multimodal imaging. In addition to biomedical imaging, peptide‐ and polypeptide‐based materials have also been endowed with therapeutic functions for disease theranostics. This review is concluded with a perspective on future peptide‐ and polypeptide‐based materials for biomedical imaging.

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“…Despite the progress made, limited examples have been reported for multiplexable enzymes imaging in a controlled manner. Most recently, the first dual enzymes‐activated “AND‐gate” probe was reported based on engineering of peptides for specific tumor imaging [10e] …”

Section: Figurementioning

confidence: 99%

“…The past two decades have witnessed significant advances in the developments of fluorescence imaging techniques for enzyme detection [8–13] . Several probes for enzymatic activity imaging have been developed for applications in fluorescence‐guided tumor detection [10e,f, 11e] . Generally, enzyme‐cleavable peptides labeled with a dye and a quencher have been widely designed for imaging of enzymatic activity through the cleavage‐induced fluorescence recovery [10] .…”

Section: Figurementioning

confidence: 99%

“…The intratumoral fluorescence signal in the TP–AP group was 2.8‐ and 3.1‐fold higher than that in the TP–nAP and mTP–AP groups, respectively. Notably, TP–AP induced a significantly enhanced tumor‐to‐background ratio compared with the single‐parameter probes (Figure S21 and S22), indicating that the AND‐gate probes are more effective for tumor imaging because the tumor is identified with multiple associated biomarkers [10e] …”

Section: Figurementioning

confidence: 99%

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A Cooperatively Activatable, DNA‐based Fluorescent Reporter for Imaging of Correlated Enzymatic Activities

et al. 2021

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The dynamic variation of the expression profile and spatial landscape of multiple enzymes are crucial factors influencing tumor progression and drug treatment. However, the comprehensive analysis of these events has been hampered by the limitations of existing imaging technologies. Here we report a cooperatively activatable, DNA‐based fluorescent reporter programmed to detect the correlated activity of dual enzymes, telomerase (TE) and apurinic/apyrimidinic endonuclease 1 (APE1), both in vitro and in vivo. The conformational change of the DNA probe can be orthogonally triggered through TE‐induced DNA elongation and APE1‐mediated specific cleavage, producing a fluorescent signal for imaging the activity of the two enzymes in an AND‐gated manner. Furthermore, we demonstrate the capability of the system for specific tumor imaging through “dual lock‐and‐key” strategy, and visualizing the correlated enzymatic activities during drug treatment of cancer.

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AND-gate contrast agents for enhanced fluorescence-guided surgery

Cited by 99 publications

References 50 publications

Interfacing Biomaterials with Synthetic T Cell Immunity

Interfacing Biomaterials with Synthetic T Cell Immunity

Advances and Perspectives of Peptide and Polypeptide‐Based Materials for Biomedical Imaging

A Cooperatively Activatable, DNA‐based Fluorescent Reporter for Imaging of Correlated Enzymatic Activities

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