Ancestry-independent fate specification and plasticity in the developmental timing of a typical<i>Drosophila</i>neuronal lineage

Gaziova, Ivana; Bhat, Krishna Moorthi

doi:10.1242/dev.027854

Cited by 14 publications

(41 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous reports have described the expression of Mid in the embryonic ectoderm, neuroblasts, and cardiomyoblasts (Buescher et al 2004;Gaziova and Bhat 2009;Leal et al 2009;Liu et al 2009;Formaz-Preston et al 2012), but did not note expression in the somatic musculature. To evaluate whether the observed muscle phenotypes correlate with Mid expression, and support the hypothesis that Mid functions in a cell autonomous manner in the musculature, we examined the pattern of Mid from stages 10-15 of myogenesis ( Figure 2).…”

Section: Muscles Form Correct Attachments In Mid Mutantsmentioning

confidence: 91%

“…Analysis of the mid and H15 mutant phenotypes utilized Df (2L)x528/Df(2L)GpdhA (Buescher et al 2004) and mid 1 /Df (2L)BSC810 (Gaziova and Bhat 2009). The breakpoints of Df(2L)BSC810 have been defined molecularly (Cook et al 2012).…”

Section: Fly Stocks and Geneticsmentioning

confidence: 99%

“…Mid and H15, orthologs of vertebrate Tbx20, play partially redundant roles during neuroblast specification (Buescher et al 2006) and axon guidance (Liu et al 2009;Manavalan et al 2013). Interestingly, the role of Mid in selecting muscle fate choice between LT3 and LT4 is analogous to its function in the nervous system, where it mediates neuroblast fate specification (Buescher et al 2006;Gaziova and Bhat 2009). Mid and H15 also promote cell fate specification and morphogenesis in the Drosophila heart (Miskolczi-McCallum et al 2005;Qian et al 2005;, where mid is both a target and a regulator of the Tin transcription factor (Qian et al 2005;Ryu et al 2011;Jin et al 2013).…”

Section: Mid and The T-box Family Of Proteinsmentioning

confidence: 99%

“…We found that there was an enrichment of b-PS-integrin at the poles of the LT muscles in both wild type and mid 1 homozygous mutant embryos (see arrows, Figure S1, C and D), suggesting that these muscles formed correct attachments to the internally located tendons. Taken together, our data are consistent with a cell autonomous role for Mid in the muscles.Mid is expressed in a subset of progenitors, founder cells, and developing muscle fibersPrevious reports have described the expression of Mid in the embryonic ectoderm, neuroblasts, and cardiomyoblasts (Buescher et al 2004;Gaziova and Bhat 2009;Leal et al 2009;Liu et al 2009;Formaz-Preston et al 2012), but did not note expression in the somatic musculature. To evaluate whether the observed muscle phenotypes correlate with Mid expression, and support the hypothesis that Mid functions in a cell autonomous manner in the musculature, we examined the pattern of Mid from stages 10-15 of myogenesis ( Figure 2).…”

mentioning

confidence: 99%

“…It is one of eight Drosophila T-box transcription factors and plays a fundamental role in cell fate choice in several differentiating tissues. In the nervous system, mutations in mid lead to the duplication of specific neurons and concomitant loss of their siblings (Buescher et al 2006;Gaziova and Bhat 2009;Leal et al 2009). Its loss impacts axon guidance (Liu et al 2009) due to misspecification of surrounding neuroblasts (Manavalan et al 2013).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Muscle Cell Fate Choice Requires the T-Box Transcription Factor Midline in Drosophila

et al. 2015

View full text Add to dashboard Cite

Drosophila Midline (Mid) is an ortholog of vertebrate Tbx20, which plays roles in the developing heart, migrating cranial motor neurons, and endothelial cells. Mid functions in cell-fate specification and differentiation of tissues that include the ectoderm, cardioblasts, neuroblasts, and egg chambers; however, a role in the somatic musculature has not been described. We identified mid in genetic and molecular screens for factors contributing to somatic muscle morphogenesis. Mid is expressed in founder cells (FCs) for several muscle fibers, and functions cooperatively with the T-box protein H15 in lateral oblique muscle 1 and the segment border muscle. Mid is particularly important for the specification and development of the lateral transverse (LT) muscles LT3 and LT4, which arise by asymmetric division of a single muscle progenitor. Mid is expressed in this progenitor and its two sibling FCs, but is maintained only in the LT4 FC. Both muscles were frequently missing in mid mutant embryos, and LT4-associated expression of the transcription factor Krüppel (Kr) was lost. When present, LT4 adopted an LT3-like morphology. Coordinately, mid misexpression caused LT3 to adopt an LT4-like morphology and was associated with ectopic Kr expression. From these data, we concluded that mid functions first in the progenitor to direct development of LT3 and LT4, and later in the FCs to influence whichever of these differentiation profiles is selected. Mid is the first T-box factor shown to influence LT3 and LT4 muscle identity and, along with the T-box protein Optomotor-blind-related-gene 1 (Org-1), is representative of a new class of transcription factors in muscle specification. KEYWORDS midline; Tbx20; founder cell; muscle identity; differentiation T HE body wall muscles of the Drosophila larva are composed of a stereotypic pattern that includes 30 unique muscle fibers (each a single myotube) per abdominal hemisegment (Bate 1990). These muscles form during embryogenesis, during which time they take on unique characteristics such as size, shape, pattern of innervation, and epidermal attachment Baylies et al. 1998;Frasch 1999;De Joussineau et al. 2012). The unique identity of each muscle fiber is conferred through overlapping signaling pathways and cell autonomous transcription factors that specify muscle progenitors Baylies et al. 1998;Frasch 1999;Tixier et al. 2010), which then undergo asymmetric cell division to generate founder cells (FCs) (Bate 1990;Carmena et al. 1998). A single FC initiates the formation of multinucleate syncytia by fusing with fusion competent myoblasts (FCMs) (Rochlin et al. 2010;Abmayr and Pavlath 2012). FCs dictate the number of cell fusion events that take place, leading to a characteristic number of nuclei for each muscle in the pattern . These fusing nuclei adopt the transcriptional profile of the original FC nucleus (Bourgouin et al. 1992;D'Alessio and Frasch 1996;Ruiz-Gomez et al. 1997;Jagla et al. 1998;Keller et al. 1998;Nose et al. 1998;Crozatier and Vincent 1999;Knirr et al. 1999;D...

show abstract

Section: Muscles Form Correct Attachments In Mid Mutantsmentioning

confidence: 91%

Section: Fly Stocks and Geneticsmentioning

confidence: 99%

Section: Mid and The T-box Family Of Proteinsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Muscle Cell Fate Choice Requires the T-Box Transcription Factor Midline in Drosophila

et al. 2015

View full text Add to dashboard Cite

show abstract

Axonal commissures in the central nervous system: how to cross the midline?

Nawabi

Castellani

2011

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Organisms with bilateral symmetry elaborate patterns of neuronal projections connecting both sides of the central nervous system at all levels of the neuraxis. During development, these so-called commissural projections navigate across the midline to innervate their contralateral targets. Commissural axon pathfinding has been extensively studied over the past years and turns out to be a highly complex process, implicating modulation of axon responsiveness to the various guidance cues that instruct axon trajectories towards, within and away from the midline. Understanding the molecular mechanisms allowing these switches of response to take place at the appropriate time and place is a major challenge for current research. Recent work characterized several instructive processes controlling the spatial and temporal fine-tuning of the guidance molecular machinery. These findings illustrate the molecular strategies by which commissural axons modulate their sensitivity to guidance cues during midline crossing and show that regulation at both transcriptional and post-transcriptional levels are crucial for commissural axon guidance.

show abstract

Response to the Dorsal Anterior Gradient of EGFR Signaling in Drosophila Oogenesis Is Prepatterned by Earlier Posterior EGFR Activation

et al. 2013

View full text Add to dashboard Cite

Spatially restricted epidermal growth factor receptor (EGFR) activity plays a central role in patterning the follicular epithelium of the Drosophila ovary. In midoogenesis, localized EGFR activation is achieved by the graded dorsal anterior localization of its ligand, Gurken. Graded EGFR activity determines multiple dorsal anterior fates along the dorsal-ventral axis but cannot explain the sharp posterior limit of this domain. Here, we show that posterior follicle cells express the T-box transcription factors Midline and H15, which render cells unable to adopt a dorsal anterior fate in response to EGFR activation. The posterior expression of Midline and H15 is itself induced in early oogenesis by posteriorly localized EGFR signaling, defining a feedback loop in which early induction of Mid and H15 confers a molecular memory that fundamentally alters the outcome of later EGFR signaling. Spatial regulation of the EGFR pathway thus occurs both through localization of the ligand and through localized regulation of the cellular response.

show abstract

Ancestry-independent fate specification and plasticity in the developmental timing of a typicalDrosophilaneuronal lineage

Cited by 14 publications

References 38 publications

Muscle Cell Fate Choice Requires the T-Box Transcription Factor Midline in Drosophila

Muscle Cell Fate Choice Requires the T-Box Transcription Factor Midline in Drosophila

Axonal commissures in the central nervous system: how to cross the midline?

Response to the Dorsal Anterior Gradient of EGFR Signaling in Drosophila Oogenesis Is Prepatterned by Earlier Posterior EGFR Activation

Contact Info

Product

Resources

About