Anatomy of the Mouse Placenta Throughout Gestation

Isaac, Sarah; Langford, Michael B.; Simmons, David G.; Adamson, S. Lee

doi:10.1016/b978-0-12-394445-0.00004-7

Cited by 8 publications

(4 citation statements)

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“…The placenta was removed and soaked in 4% paraformaldehyde (PFA) on a horizontal shaker overnight and embedded with paraffin [ 30 ]. Following this step, the placental section was dyed using hematoxylin and eosin (H&E).…”

Section: Methodsmentioning

confidence: 99%

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Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast

et al. 2022

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Section: Methodsmentioning

confidence: 99%

“…Following this step, the placental section was dyed using hematoxylin and eosin (H&E). According to the structure, the placenta was divided into four zones: decidua (dec), junctional zone (jz), labyrinth (lab), and chorionic plate (cp) [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast

et al. 2022

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“…Microphotographs were taken of areas of the labyrinth free of artefacts at 200× magnification. To assess the fetal vascular channels, immunohistochemistry for CD34 was performed using rat anti-murine CD34 (ab8158; Abcham, Cambridge, UK) and Vector-AB anti-rat kit [ 32 ]. Using ImageJ software, 10 randomly chosen vascular channels lined by CD34 positive endothelial cells were outlined and the pixel number per luminal area recorded.…”

Section: Methodsmentioning

confidence: 99%

Maternal Influenza A Virus Infection Restricts Fetal and Placental Growth and Adversely Affects the Fetal Thymic Transcriptome

Campen

Bishop

Abrahams

et al. 2020

Viruses

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Maternal influenza A viral infections in humans are associated with low birth weight, increased risk of pre-term birth, stillbirth and congenital defects. To examine the effect of maternal influenza virus infection on placental and fetal growth, pregnant C57BL/6 mice were inoculated intranasally with influenza A virus A/CA/07/2009 pandemic H1N1 or phosphate-buffered saline (PBS) at E3.5, E7.5 or E12.5, and the placentae and fetuses collected and weighed at E18.5. Fetal thymuses were pooled from each litter. Placentae were examined histologically, stained by immunohistochemistry (IHC) for CD34 (hematopoietic progenitor cell antigen) and vascular channels quantified. RNA from E7.5 and E12.5 placentae and E7.5 fetal thymuses was subjected to RNA sequencing and pathway analysis. Placental weights were decreased in litters inoculated with influenza at E3.5 and E7.5. Placentae from E7.5 and E12.5 inoculated litters exhibited decreased labyrinth development and the transmembrane protein 150A gene was upregulated in E7.5 placentae. Fetal weights were decreased in litters inoculated at E7.5 and E12.5 compared to controls. RNA sequencing of E7.5 thymuses indicated that 957 genes were downregulated 2-fold including Mal, which is associated with Toll-like receptor signaling and T cell differentiation. There were 28 upregulated genes. It is concluded that maternal influenza A virus infection impairs fetal thymic gene expression as well as restricting placental and fetal growth.

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“…In this scenario, an organ stands out as a key player for fetal development: the placenta. It is composed of distinct layers, each one with a specific function: endocrine and metabolic (junctional zone) or maternalfetal exchanges (labyrinthtrophoblast layer) (Isaac et al, 2014).…”

Section: Low Protein Dietmentioning

confidence: 99%

Intrauterine growth restriction: screening and diagnosis using animal models

et al. 2019

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Intrauterine growth restriction (IUGR) is a serious condition of multifactorial origin, mainly caused by maternal malnutrition, multiple gestation associated with nutrient competition, abuse of nocive substances and infections. The diagnosis of such syndrome is complex, as its own manifestations can mask its occurrence, requiring a thorough assessment of body weight and size. Moreover, it is not responsive to any kind of treatment. There is evidence that IUGR may predispose the individual to several pathologies, such as diabetes, hypertension and metabolic syndrome in adulthood, and it has also been linked to thrifty phenotype hypothesis. Thus, a healthy lifestyle is needed to better prevent those pathologies. Given the world high prevalence and importance of IUGR, mainly in developing countries, this review is focused on discussing how different animal models contribute to the biological screening and diagnosis of this condition.

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Anatomy of the Mouse Placenta Throughout Gestation

Cited by 8 publications

References 0 publications

Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast

Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast

Maternal Influenza A Virus Infection Restricts Fetal and Placental Growth and Adversely Affects the Fetal Thymic Transcriptome

Intrauterine growth restriction: screening and diagnosis using animal models

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