Anatomy and Histology of the Genital Tract, Spermatheca, Spermathecal Duct and Glands of Apis Mellifica Queens (Hymenoptera : Apidae)

Camargo, João Maria Franco de; Mello, Maria Luiza S.

doi:10.1051/apido:19700401

Cited by 18 publications

(18 citation statements)

References 4 publications

(4 reference statements)

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“…Since in A. s. piriventris the spermatheca is not divided into two distinct portions and the epithelium producing the secretion is located internally to the reservoir, the secretion is produced and discharged directly by exocytosis, with vesicles from the apical portion of the cytoplasm of secretory cells being released into the lumen of the spermatheca, without the participation of secretory cells externally to the organ. The same process has already been observed and described for the spermatheca of Rhodnius prolixus [20] . In A. laevigata, A. s. sexdens, A. coronatus and C. muelleri, the external secretory cells form a central collecting canaliculus, in which intracellular canaliculi open and collect the secretion produced by these cells, as well as transport them to the lumen of the reservoir.…”

Section: Histology and Histochemistrysupporting

confidence: 80%

Morpho-physiological Differences of the Spermatheca of Attini Ants (Hymenoptera: Myrmicinae)

Ortiz¹,

Camargo-Ma²

2006

American J. of Agricultural and Biological Sciences

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The insect's spermatheca store the spermatozoa from the time of copulation until the fertilization of the eggs and consists of a sac, usually associated to glands. The ant species Atta laevigata, Atta sexdens sexdens, Atta sexdens piriventris, Acromyrmex coronatus and Cyphomyrmex muelleri are considered pests, as they cause economics losses to several crops. The spermatheca of these species were examined by scanning electron microscopy, histological and histochemical techniques. Their morphology resembled that of a bilobular pouch partially jointed in the central portion. In A. coronatus and C. muelleri it is flatter in shape than those of other species. The external spermathecal gland, groups of secretory cells, was located at the joining point of the two spermathecal lobes of the reservoir. This was observed in A. laevigata, A. s. sexdens and A. coronatus, like a group of cells, while in C. muelleri, like filaments. An external associated gland was not observed in A. s. piriventris, instead secretory cells were arranged in a simple prismatic epithelium internally lining the spermathecal lumen. This epithelium was also observed in A. s. sexdens, although it was restricted to the joining point of the spermathecal lobes. The presence of an internal secretory epithelium in A. s. piriventris and A. s. sexdens suggests that their physiology for the maintenance of spermatozoa in viable conditions for egg fertilization might differ from A. laevigata, A. coronatus and C. muelleri, in which only an external spermathecal gland was observed.

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Section: Histology and Histochemistrysupporting

confidence: 80%

Morpho-physiological Differences of the Spermatheca of Attini Ants (Hymenoptera: Myrmicinae)

Ortiz¹,

Camargo-Ma²

2006

American J. of Agricultural and Biological Sciences

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“…Early-mated and one-year old egg-laying queens (three of each) purchased from local beekeepers at Faria Lemos (Minas Gerais state, Brazil) (20°47' 22.20" S and 42°5' 38.82" W) were also examined for comparative purposes. Although there are reports of honeybee queens living up to five years (Butler 1954), the 1.5-year age used here has been considered to be an indicator of aging processes in queens; indeed, this age is considered the mean longevity for queen honeybees used for beekeeping purposes in Brazil (Warwick E. Kerr, 1968-personal communication, apud Camargo andMello 1970). In tropical American regions, it has been recommended that honeybee colonies be requeened every 6-9 months, as less than 28.1 % out of the inseminated queens have been found to remain in their hives more than 12 months after being introduced into a colony (Guzman-Novoa et al 1998).…”

Section: Methodsmentioning

confidence: 99%

“…The sperm cells stored in the spermatheca are nourished and protected from oxidative stress and activated by a secretion produced in the spermathecal glands (Laidlaw 1944;Camargo and Mello 1970;Verma 1974;Weirich et al 2002;Klenk et al 2004;den Boer et al 2009). The spermathecal glands are a pair of long and convoluted structures, consisting of a single secretory epithelium and an intima layer, and are located over the latero-anterior surface of the spermatheca.…”

Section: Introductionmentioning

confidence: 99%

“…The spermathecal glands are a pair of long and convoluted structures, consisting of a single secretory epithelium and an intima layer, and are located over the latero-anterior surface of the spermatheca. These glands unite into a common duct, which opens on the antero-ventral midline of the organ (Camargo and Mello 1970). The intima nuclei are positioned laterally to each canaliculum, which collects the glandular secretion intracytoplasmically from each secretory epithelial cell and passes it to the glandular lumen (Camargo and Mello 1970).…”

Section: Introductionmentioning

confidence: 99%

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Feulgen-DNA content and chromatin organization in the spermathecal glands of Apis mellifera (Hymenoptera, Apoidea) queens with aging

Maldanis¹,

Falco²,

Aguirra³

et al. 2014

Apidologie

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-The secretions from the spermathecal glands in honeybee queens contribute to maintaining the viability of spermatozoa stored in the spermatheca. This viability is reduced as the queen ages. Here, we investigated whether DNA content changes and chromatin remodeling, that could suggest changes in cellular functions, occur in the spermathecal glands of Apis mellifera queens with aging. Feulgen-stained glands from early-mated and old queens were studied by image analysis. Increased polyploidization, heterogeneously distributed chromatin coarseness, and no cell death signs were verified in the spermathecal glands of aged queens. These results, compared with published data for other cellular systems, are suggestive of alterations in the spermathecal gland cells upon aging, possibly affecting their secretory fluid production. In consequence, sperm cell protection in the spermatheca would be less effective.queen / spermathecal glands / polyploidy / chromatin / aging

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“…Usually osmiophilic and PAS-positive materials appear juxta-nuclearly and these coalesce apically to be subsequently transported to the lumen of the sperrnatheca by means of cuticle lined ductules (Clements and Potter 1967;De Camargo and Mello 1970;Poole 1970). It has been claimed by Lensky and Allimot (1969) that some fractions of the haemolyrnph proteins migrate to the sperrnathecal fluid in honey-bee.…”

Section: Introductionmentioning

confidence: 99%

Electron microscopic study of the spermatheca ofGesonula punctifrons (Acrididae: Orthoptera)

Pal

Ghosh

1982

Proc Ani Sci

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The present transmission electron microscopic study of the spermatheca of a common Indian grasshopper, Gesonula punctifrons, has highlighted the presence of the glandular secretory cells (SaC) and ductule cell (DC) in the spermathecal epithelium and additionally the occurrence of muscle cells, tracheoles and haemocytes. Both the former cell types are secretory in nature and probably their discharges in the lumen of the cuticle-lined sperrnathecal duct or ductule vary in their chemical nature. The ultrastructural evidence gives ample support to a concept of a lysosomal control of the secretory materials prior to their liberation in the lumen. The characteristic features of the plasma membranes of the secretory cells clearly suggest their involvement in the transepithelial transport of ions and smaller molecules across the basement membrane. A neuronal supply to the sperrnathecal wall is yet to be demonstrated to explain the filling in and out of the male gametes by this organ.

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Anatomy and Histology of the Genital Tract, Spermatheca, Spermathecal Duct and Glands of Apis Mellifica Queens (Hymenoptera : Apidae)

Abstract: SUMMARYThe genital chamber, the spermatheca and the spermathecal duct and glands were studied by classical methods of common light and phase microscopy. Some histochemical reactions were also employed. Results were compared with published data.1.

Cited by 18 publications

References 4 publications

Morpho-physiological Differences of the Spermatheca of Attini Ants (Hymenoptera: Myrmicinae)

Morpho-physiological Differences of the Spermatheca of Attini Ants (Hymenoptera: Myrmicinae)

Feulgen-DNA content and chromatin organization in the spermathecal glands of Apis mellifera (Hymenoptera, Apoidea) queens with aging

Electron microscopic study of the spermatheca ofGesonula punctifrons (Acrididae: Orthoptera)

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