“…However, there have been no studies to date on the impact of benzodiazepine on mollusk reproduction, despite freshwater mollusks, and gastropods in particular, being regarded as reference organisms for ecotoxicological studies (Amorim et al, 2019;Tallarico, 2016) due to their widespread presence in continental surface waters, their benthic habitat and primary consumer status. Moreover, freshwater gastropod reproduction has been extensively studied for almost a century (Crabb, 1927), with detailed descriptions extending from the structure and characteristics of the reproductive system (Duncan, 1958;Ohbayashi-Hodoki et al, 2004;Tair-Abbaci et al, 2017), to mating and egglaying behaviors (De Boer et al, 1996;Ter Maat, 1992) and the associated nervous and endocrine regulation (Koene, 2010;Morishita et al, 2010). Moreover, gastropod egg masses are transparent, making it easy to observe and count eggs, facilitating monitoring.…”