Anatomical, histological and immunohistochemical study of testicular development in Columba livia (Aves: Columbiformes)

Olea, Gabriela Beatriz; Aguirre, Marina Laura; Lombardo, Daniel Marcelo

doi:10.1016/j.acthis.2018.05.010

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…Tissues were fixed in Bouin's solution for 12 h and preserved in 10% formalin. The samples were processed for conventional histology, according to Olea et al (2018). The samples were embedded in paraffin to prepare histological blocks.…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemical analysis was performed on one specimen from each species captured in each season (n = 4 per species), following the protocol proposed by Olea et al (2018Olea et al ( , 2019, to detect the presence of the PCNA and S100 proteins. Samples embedded in paraffin were sectioned at 2 μm thick, deparaffinised in two xylene baths, and hydrated with decreasing concentrations of alcohol (100 , 90 I, 90 II, 90 III, 80 , 70 , 50 and distilled water).…”

Section: Immunohistochemistry For Proliferating Cell Nuclear Antigen ...mentioning

confidence: 99%

“…Integument sections of glandular pores from each specimen were fixed in Bouin's solution and preserved in 10% buffered formalin for histological procedures or scanning electron microscope (SEM) analysis; some samples were frozen for lipid detection. The samples were observed and analysed using binocular (Leica DCC-380X) and SEM (SEM-JEOL JSM-5800 LV) microscopes, following the protocol by Olea et al (2018).…”

Section: Macroscopic and Microscopic Analysismentioning

confidence: 99%

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Comparative study of the gular gland of three species of Molossidae bats (Mammalia: Chiroptera) from South America

Rodriguez

Olea

Aguirre

et al. 2023

The Anatomical Record

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The gular gland is a skin gland located in the suprasternal region of adult males of some bat families. Knowledge of the morphology and functional aspects of these gland types is often limited. This study aimed to describe the structure and composition of the gular glands of three molossid species (Eumops patagonicus, Molossus fluminensis and Molossus molossus) with respect to their reproductive activity and to define the mechanism involved in secretion release. Different histological, histochemical and immunohistochemical techniques were used to achieve these goals. The results revealed that the size and composition of this gland are variable and are mostly related to the lipid content during the reproductive season. The results also documented, for the first time, the occurrence of mechanoreceptors associated with the surface of the glandular duct by detecting an S100 protein, indicating that an external stimulus activates secretion. Previous studies on other species have classified the gland using obsolete criteria; hence, we adopted a new classification of adenomeres in this study. Moreover, we investigated the gland secretion mechanism previously proposed. This study defines the implications of this gland in the reproduction of this species. Our preliminary interpretation of the function of the gular gland is that it is a cutaneous exocrine gland activated by mechanoreceptors involved in the reproductive behaviour of the Molossidae family.

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Section: Methodsmentioning

confidence: 99%

Section: Immunohistochemistry For Proliferating Cell Nuclear Antigen ...mentioning

confidence: 99%

Section: Macroscopic and Microscopic Analysismentioning

confidence: 99%

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