Expression of GnRH receptor and 3βHSD during meiosis and foliculogénesis in Columba livia (Aves: Columbiformes): Histological and immunohistochemical analysis

Olea, Gabriela Beatriz; Carou, María Clara; Aguirre, Marina Laura; Lombardo, Daniel Marcelo

doi:10.1016/j.ygcen.2019.113230

Cited by 5 publications

(9 citation statements)

References 28 publications

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“…The antibody was incubated at a working dilution of 1:50, for 120 minutes at An Acad Bras Cienc (2021) 93(2) e20190841 4 | 11 37 °C, and subsequently revealed according to the indirect protocol of "L-streptoavidin-biotin" -HRP (DAKO K0690). PCNA is a phylogenetically conserved protein and said antibody had been tested in many species of anurans, birds, and mammals (Carou et al 2017, Olea et al 2018, 2020, Scaia 2019.…”

Section: Immunohistochemical Studies To Determine the Immunodetection...mentioning

confidence: 99%

Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae): morphological, histological and immunohistochemical analysis

Olea

Cheij

Boccioni

et al. 2021

An. Acad. Bras. Ciênc.

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To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson's trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.

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Section: Immunohistochemical Studies To Determine the Immunodetection...mentioning

confidence: 99%

Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae): morphological, histological and immunohistochemical analysis

Olea

Cheij

Boccioni

et al. 2021

An. Acad. Bras. Ciênc.

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“…We showed that Oct-4Oct4 and N-cadherinpositive cells were detected in the yolk sac membrane outside the blood vessels, on the genital ridges, and the dorsal mesentery, suggesting that PGCs migrate through the yolk sac membrane and the dorsal mesentery. GnRH receptors (GnRHR) have been described in several vertebrate species (Canosa et al, 2007;Molés et al, 2007;Olea et al, 2020). Expression of GnRHR genes varies with sex and reproductive status, due in part to feedback from FSH and LH and steroids, including estradiol, testosterone, and maturation-inducing hormone (Molés et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Expression of GnRHR genes varies with sex and reproductive status, due in part to feedback from FSH and LH and steroids, including estradiol, testosterone, and maturation-inducing hormone (Molés et al, 2007). In previous works, it was possible to show that GnRHR and 3βHSD reach its highest immunodetection at the time when the gonads are fully developed and with a differentiating follicle (Olea et al, 2020)…”

Section: Discussionmentioning

confidence: 99%

Early gonadogenesis in Columba livia (birds: Columbiformes): Migration, colonization, and differentiation of germ cells

Olea,

Aguirre,

Lombardo

2023

Dev Growth Differ

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In birds, primordial germ cells (PGCs) use the bloodstream to travel to a specific region, where the cells undergo extravasation followed by intrastromal migration to the gonadal crest for further colonization. Currently, DDX4, SSEA1 and Oct4 are used to identify germ cells. Other germline cell‐associated molecules are N‐cadherin, GnRHR, and 3βHSD, which have been used in mice and birds during gonadal development; however, its role in early gonadogenesis in birds is poorly described. This study aimed to evaluate the differential immunodetection of N‐cadherin binding molecule, Oct4 pluripotency protein, GnRHR receptor and 3βHSD enzyme in Columba livia embryos during migration colonization of PGCs in the crest. gonadal and early gonadogenesis. These markers were revealed by immunohistochemistry in histological preparations of C. livia corresponding to stages (S) 15 to S40. Immunodetection of N‐cadherin, Oct4, GnRHR and 3βHSD in the germ line of C. livia allowed the identification of PGCs in the yolk sac membrane at the level of the splanchnic mesoderm during migration to the genital crest and its colonization. In the same way, it was possible to characterize and localize PGCs during early gonadogenesis. This study in C. livia demonstrates that Oct4, N‐cadherin, GNRHR, and 3βHSD are immunodetected in PGCs and could be used as potential germline cell markers during cell migration out of blood vessels, colonization in the genital crest and early gonadogenesis. Furthermore, this study could be used as a novel general model to understand the early gonadogenesis in altricial species.This article is protected by copyright. All rights reserved.

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“…HSD-3β is one of the key enzymes in the steroidogenesis pathway, whose expression steadily increases in granulosa cells of growing hierarchical follicles (50,51). Through a series of endocrine processes in the embryonic ovary, HSD-3β and gonadotropins are likely to promote the initiation of germ cell meiosis (52). HSD17B2 is an endoplasmic reticulum enzyme that inactivates hormones.…”

Section: Discussionmentioning

confidence: 99%

Integrated ONT Full-Length Transcriptome and Metabolism Reveal the Mechanism Affecting Ovulation in Muscovy Duck (Cairina moschata)

Xiang

Niu

et al. 2022

Front. Vet. Sci.

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Ovulation is a complicated physiological process that is regulated by a multitude of different pathways. In comparison to mammalian studies, there are few reports of ovulation in Muscovy ducks, and the molecular mechanism of ovarian development remained unclear. In order to identify candidate genes and metabolites related to Muscovy duck follicular ovulation, the study combined Oxford Nanopore Technologies (ONT) full-length transcriptome and metabolomics to analyze the differences in gene expression and metabolite accumulation in the ovaries between pre-ovulation (PO) and consecutive ovulation (CO) Muscovy ducks. 83 differentially accumulated metabolites (DAMs) were identified using metabolomics analysis, 33 of which are related to lipids. Combined with data from previous transcriptomic analyses found that DEGs and DAMs were particularly enriched in processes including the regulation of glycerophospholipid metabolism pathway, arachidonic acid metabolic pathway and the steroid biosynthetic pathway. In summary, the novel potential mechanisms that affect ovulation in Muscovy ducks may be related to lipid metabolism, and the findings provide new insights into the mechanisms of ovulation in waterfowl and will contribute to a better understanding of changes in the waterfowl ovarian development regulatory network.

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Expression of GnRH receptor and 3βHSD during meiosis and foliculogénesis in Columba livia (Aves: Columbiformes): Histological and immunohistochemical analysis

Cited by 5 publications

References 28 publications

Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae): morphological, histological and immunohistochemical analysis

Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae): morphological, histological and immunohistochemical analysis

Early gonadogenesis in Columba livia (birds: Columbiformes): Migration, colonization, and differentiation of germ cells

Integrated ONT Full-Length Transcriptome and Metabolism Reveal the Mechanism Affecting Ovulation in Muscovy Duck (Cairina moschata)

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