Anatomical Features of Vibrio fetus: Electron Microscopic Survey

Ritchie, A. E.; Keeler, R. F.; Bryner, John H.

doi:10.1099/00221287-43-3-427

Cited by 52 publications

(24 citation statements)

References 29 publications

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“…4A). Similar depressions have been found in thin sections of Campylobacter jejuni (2), Campylobacter fetus (formerly Vibrio fetus) (19), and Wolinella recta (17), suggesting that outer membrane, basal disk, and peptidoglycan layer are close together, at least in their central part around the flagellum. This is supported by electron micrographs of spheroplasts from W. succinogenes (Fig.…”

Section: Discussionmentioning

confidence: 53%

“…6) and Spirillum volutans (25). In several electron micrographs of various bacteria, similar but not so differentiated cytoplasmic areas of high electron density may be observed, for example, in C. jejuni (2) and C. fetus (19). From the elements described above, we propose a hypothetical model of the HBB complex with respect to the cell wall (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Location of the basal disk and a ringlike cytoplasmic structure, two additional structures of the flagellar apparatus of Wolinella succinogenes

Schuster

Bäeuerlein

1992

J Bacteriol

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The basal body of Wolinella succinogenes consists of a central rod, a set of two rings (L and P rings), a basal disk front 70 to 200 nm in diameter, and a terminal knob. In negatively stained preparations of flagellar hook-basal body complexes, some disks remain fixed perpendicularly to the grid and show that such a disk is located on the distal side of the P ring. The basal disks have been isolated with and without the P ring; in both cases there is a hole in the center of the disk. The diameter of the disk is smaller in the presence of the P ring. The L-P ring complex is therefore assumed to be a bushing for the rod. Thin sections of whole bacteria and spheroplasts reveal that the disk is attached to the inner surface of the outer membrane. At the insertions of the flagellar hook-basal body-basal disk complexes, depressions are visible in negatively stained preparations of whole bacteria and spheroplasts. A new ringlike structure is connected to an elongation of the basal body into the cytoplasm in both preparations. Its diameter (60 nm) is larger than that of the M ring. A heavily stained compartment can be seen in between the new ringlike structure and the basal disk, which may be formed by the energy transducing units.For a long time the entity called the intact flagellum (6-8) was thought to be the essential rotary device of the flagellar motor. In Escherichia coli and Salmonella typhimurium, this assembly consists of the flagellar filament, the hook, and the basal body. The basal body appears as a set of four rings coaxial with the rod. Two rings are very likely to be correlated to membranes: the L ring to the outer membrane and the M ring to the inner membrane. The P ring is in the periplasmic space and is assumed to interact with the peptidoglycan layer. The S ring is clearly visible in negatively stained electron micrographs in both bacteria, but from genetic, biochemical, and physiological studies no indications for its existence emerged until now (10, 11). In an extensive search for flagellar and motility mutants with the paralyzed or switch-defective phenotype, none mapped to the gene of the M ring (fliF) (10); all mapped to five genes encoding proteins that are not present on isolated basal bodies. Three of them (fiG, fliM, fliN) encode flagellar components that participate in both energy transduction and switching (15), whereas the other two (motA, motB) encode proteins that appear to function in energy transduction only (5,18,21,22). It is therefore unlikely that the M ring is the active rotor of the bacterial flagellar apparatus. Furthermore, it was proposed about 13 years ago by Coulton and Murray (3, 4) that essential structures of the flagellar apparatus were lost during the procedures for isolation of intact flagella. They found circlets of about 15 particles around the rod in the cytoplasmic membrane and outer membraneassociated large disks, called concentric membrane rings, in Aquaspirillum serpens.From Wolinella succinogenes, an anaerobic, gram-negative bacterium with one polar flagellum,...

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Location of the basal disk and a ringlike cytoplasmic structure, two additional structures of the flagellar apparatus of Wolinella succinogenes

Schuster

Bäeuerlein

1992

J Bacteriol

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“…1). Ultrastructurally, both C. jejuni and C. coli resembled C. fetus (Werner, Levy and Spurlock, 1961;Ritchie, Keeler and Bryner, 1966) and recently examined isolates of C. jejuni from man (Pead, 1979). No surface appendages other than flagella were present, nor were the flagella sheathed.…”

Section: Resultsmentioning

confidence: 99%

Role of DNA and bacteriophage in campylobacter auto-agglutination

Ritchie

Bryner

Foley

1983

Journal of Medical Microbiology

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SUMMARY. Auto-agglutinated and non-agglutinated cells of Campylobacter jejuni and C. coli were examined by transmission electronmicroscopy in phosphotungstate negative stain. Agglutination was induced by three factors (I) extracellular DNA, (2) an aggregated protein, probably a bacteriophage precursor, and (3) free phage-tail sheaths. Auto-agglutinated cells were often "leaky," with a mantle of adhering DNA. About 80% of the auto-agglutinated cells could be resuspended after treatment with DNAase. Flagella were loosely embedded in protein aggregates, especially in phage-infected cultures. They were clumped in a side-by-side arrangement by free phage-tail sheaths. These findings suggest that auto-agglutination could be minimised in suspensions of organisms intended for use in agglutination tests by harvesting early logarithmic-phase cells containing no more than a low phage population. The most common C. jejuni phage had a contractile tail, a head diameter of 60-70 nm, and an overall length of 180-2 10 nm. A phage isolated from C. jejuni strain 1590 was morphologically identical with C. coli phage.

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“…The vibrio had morphologic features similar to V. ,fetus [8]. Those features used in identifying the agents seen here in sections as vibrio were (1) comma-shaped to undulating body form; (2) irregular multilaminated, loosely applied cell wall ( fig.…”

Section: Electron Microscopymentioning

confidence: 93%

Intraepithelial Vibrio Associated with Acute Typhlitis of Young Rabbits

et al. 1974

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Abstract. A retrospective morphologic study was conducted on tissues from weanling laboratory rabbits with diarrheal disease of unknown cause. The consistent lesion was in the cecum and consisted of exaggerated degeneration and loss and replacement of surface absorptive cells. This was accompanied by hyperplasia of crypt epithelial cells and acute diffuse inflammation of the lamina propria and submucosa. Numerous bacteria, identified morphologically as vibrio sp., were in surface epithelial cells in the cecum of affected rabbits. This was demonstrated in 10 of 12 affected rabbits via light microscopic examination of Levaditi-stained histologic sections. The occurrence, intraepithelial location, and identification of the vibrio, as well as the associated cytopathology, was confirmed in four of the six ceca from affected rabbits examined by electron microscopy. Lesions were not seen in cecal mucosa of six healthy weanlings from the colony. Vibrio was seen in the intestinal and cecal lumina of one control by both Levaditi and electron microscopic methods; none had invaded the mucosa, however. The possibilities that vibrio had either a primary or secondary causal association with acute typhlitis and that the disease had a pathogenesis similar to that of primate shigellosis are discussed.

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Anatomical Features of Vibrio fetus: Electron Microscopic Survey

Cited by 52 publications

References 29 publications

Location of the basal disk and a ringlike cytoplasmic structure, two additional structures of the flagellar apparatus of Wolinella succinogenes

Location of the basal disk and a ringlike cytoplasmic structure, two additional structures of the flagellar apparatus of Wolinella succinogenes

Role of DNA and bacteriophage in campylobacter auto-agglutination

Intraepithelial Vibrio Associated with Acute Typhlitis of Young Rabbits

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