Anatomical and biochemical changes in leaves of Vitis labrusca L. cv. Niagara Rosada in response to infection by Elsinoë ampelina Shear

Braga, Zélia Valente; Muniz, L.; Manarim, Gislene Roberta; Aguiar, Cláudio Lima de; Appezzato‐da‐Glória, Beatriz

doi:10.1007/s40415-020-00677-6

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…The epidermal and mesophyll cells of infected R. occidentalis and R. niveus accumulated polyphenols that may vastly hamper the development of haustoria. The accumulation of phenolic compounds as a response to pathogen infection has been confirmed for several pathosystems [ 19 , 20 , 24 , 69 , 81 ]. Their role is not only associated with the chemical activity but also with the structural function.…”

Section: Discussionmentioning

confidence: 99%

“…Raspberries are economically relevant crops in almost all continents [ 17 , 18 ], thus diseases affecting these plants can not be neglected. Histopathological and biochemical studies have allowed an understanding of the important pathosystems [ 19 , 20 , 21 , 22 , 23 , 24 ] and have shed light on studies of the disease cycle and its epidemiology [ 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum

Dias

Spósito

Tessmer

et al. 2023

JoF

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Late leaf rust is a fungal disease in raspberries caused by Aculeastrum americanum (Farl.) M. Scholler U. Braun (syn. Thekopsora americana (Farl.) Aime McTaggart) leading to early defoliation and yield losses. Red raspberries (Rubus idaeus L.) are susceptible to this pathogen, although this susceptibility varies among cultivars. In contrast, black raspberries were previously reported to be more resistant (Rubus occidentalis L.) and immune (Rubus niveus Thunb.) to this pathogen, raising their importance in plant breeding programs. However, what features make them respond differently to the same pathogen? In this study, we characterize for the first time the pre- and post-formed structural and biochemical defense mechanisms of R. idaeus cv. Autumn Bliss, R. occidentalis and R. niveus. Ultrastructural and histopathological analyses were used to uncover the interactions between these raspberries and A. americanum. The ultrastructural results indicate that the pathogen germinates on both leaf surfaces but can only form appressoria on the stomata. Although the three raspberry species were infected and colonized by A. americanum, a clear difference in susceptibility was observed between them. A compact mesophyll, pre- and post-formed phenolic compounds, and post-formed pectic compounds were the main plant defense mechanisms against fungal colonization. These findings provide new information about raspberries’ defense mechanisms in response to A. americanum and elucidate the interactions occurring in these pathosystems.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum

Dias

Spósito

Tessmer

et al. 2023

JoF

Self Cite

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“…In the leaves of 'Niagara Rosada', a susceptible variety with V. labrusca and V. vinifera background, vesicular-like structures on the epidermis were observed 2 days after E. ampelina inoculation (Braga et al 2019). Furthermore, in the susceptible V. vinifera variety, Thompson Seedless, E. ampelina hyphae infected the leaves within 24 hours after inoculation, subsequently growing through the palisade tissues and penetrating the mesophyll cells between 48 and 72 hours after inoculation (Han et al 2020), resulting in the collapse and death of the epidermal and mesophyll cells (Braga et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Resistance of Vitis germplasm to anthracnose in Southern Brazil

Menon,

Modesto,

Bogo¹

et al. 2023

Preprint

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Anthracnose is a fungal disease that affects all parts of grapevine in warm and humid regions of the world. The present study aimed to assess 13 Vitis spp. germplasm accessions for resistance to grapevine anthracnose, caused by E. ampelina in southern Brazil, during the 2014/2015 and 2015/2016 growing seasons. Anthracnose incidence and severity were assessed biweekly under natural infection from budburst (October – BBCH-09) until flowering (November – BBCH-63). The accessions were compared based on the following epidemiological parameters: a) beginning of symptoms appearance (BSA), b) time to reach the maximum disease incidence and severity (TRMDI and TRMDS), c) maximum disease incidence and severity (Imax, Smax), and d) areas under incidence and severity disease progress curves (AUIDPC and AUSDPC). Additionally, histological analyses and quantification of hydrogen peroxide production were conducted with the less and high susceptible accessions to anthracnose. In general, there was significant difference in estimated epidemiological parameters according to accessions. The principal parameters that differentiated accessions were Imax, Smax, AUIDPC, and AUSDPC. V. berlandieri, V. shuttleworthii, V. candicans, and Baco 1 and V. thunbergii and V. betulifolia were the less and high susceptible accessions to anthracnose, respectively. V. thunbergii and V. betulifolia showed the highest Imax, Smax, AUIDPC, and AUSDPC when compared to the less susceptible V. berlandieri, V. shuttleworthii, and V. candicans. Additionally, V. betulifolia exhibited higher cell death in leaf tissues around the E. ampelina infected points than V. shuttleworthii. The variation in susceptibility level to anthracnose among accessions can be utilized in further grapevine breeding programs.

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“…The total flavonoids concentration of the wort samples were measured according to Mabry et al (2012) with modifications described by Braga et al (2021). The samples were diluted with ethanol (70 % v v -1 ) 50 times and measured in triplicate.…”

Section: Total Flavonoidsmentioning

confidence: 99%

Wort disinfection treatment with electron beam for bioethanol production

Calegari

Silva

et al. 2023

Sci. agric. (Piracicaba, Braz.)

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Microbial contamination of the wort during the fermentation process causes significant losses in ethanol production worldwide and creates a dependence of the industry on chemicals and antibiotics to control contamination. Therefore, this study used electron beam (e-beam) to disinfect wort from sugarcane (Saccharum officinarum L.) molasses and investigate the bioethanol fermentation. Four treatments (T0 -T3) were carried out using ionizing doses of radiation through the electron accelerator: 0 (control), 10, 20, and 40 kGy. Total mesophiles, total bacteria, sucrose, glucose, fructose, phenolics, flavonoids, hydroxymethylfurfural (5-HMF), and Furfural were measured. An alcoholic fermentation assay was performed after the irradiation process. The irradiated treatments showed no inversion of sugars and formation of the inhibitory by-products flavonoids, furfural and 5-HMF, except for the phenolic compounds. The lower dose tested (10 kGy) reduced more than 99.9 % of the total mesophiles and more than 99.99 % of the total bacteria in the substrate. In the fermentation, the irradiated worts presented similar (p > 0.05) yields (92, 93, and 94 %) and ethanol productivity levels (0.89, 0.88, and 0.87 g L -1 h -1 , for T1, T2, and T3 respectively). However, all treatments presented higher yields and productivity (p < 0.05) when compared to the control (88 % and 0.85 g L -1 h -1 ), highlighting the possible use of e-beam in wort fermentation at a lower dose (10 kGy). This allows reduction in losses caused by microbial contamination, besides increasing fermentation yield and productivity with lower energy consumption.

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Anatomical and biochemical changes in leaves of Vitis labrusca L. cv. Niagara Rosada in response to infection by Elsinoë ampelina Shear

Cited by 3 publications

References 31 publications

Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum

Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum

Resistance of Vitis germplasm to anthracnose in Southern Brazil

Wort disinfection treatment with electron beam for bioethanol production

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