ANATI QUANTI: software de análises quantitativas para estudos em anatomia vegetal

Aguiar, T.V.; Sant’Anna-Santos, Bruno Francisco; Azevedo, Aristéa Alves; Ferreira, Ricardo

doi:10.1590/s0100-83582007000400001

Cited by 74 publications

(44 citation statements)

References 14 publications

(10 reference statements)

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“…The stomata were counted and the stomatal index (SI) was calculated in the Anati Quant 2® UFV software (Aguiar et al 2007). Three individuals from each phytophysiognomy were analyzed; four sections per individual, thus totaling 12 sections.…”

Section: Methodsmentioning

confidence: 99%

Leaf anatomy of Qualea parviflora (Vochysiaceae) in three phytophysiognomies of the Mato Grosso State, Brazil

Ariano

Silva

2016

Acta Amaz.

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Leaves have a variety of morphological and anatomical characters mainly influenced by climatic, edaphic and biotic factors. The aim of this study was to describe the anatomical leaf traits of Qualea parviflora from three phytophysiognomies. The studied phytophysiognomies were Amazon Savannah on rocky outcrops (ASR), Transition Rupestrian Cerrado (TRC), and Cerradão (CDA). Freehand sections of the leaf blade were made and stained with 0.5% astra blue and with basic fuchsin. From the adaxial and abaxial leaf surface, freehand paradermal sections were made for epidermis analysis. The Jeffrey´s method, with modifications, was used in the epidermis dissociation process. The samples from the TRC phytophysiognomy had relatively smaller ordinary epidermal cells, higher abundance of trichomes, and mesophyll with few intercellular spaces, in comparison to the other phytophysiognomies. The leaves from the ASR phytophysiognomy had higher stomatal index (SI = 21.02), and five to six layers of sclerenchyma surrounding the midrib vascular bundle. The secondary vascular bundles had thicker cell walls and the bundle sheath extended up to the epidermal tissue of both leaf sides. Leaves from the CDA phytophysiognomy had mesomorphic environmental traits, such as a thinner cuticle. It is concluded that trees from ASR and TRC phytophysiognomies have xeromorphic traits following the environmental conditions where they occur. KEYWORDS: Adaptations, Amazon, Cerrado, Environmental conditions. Anatomia foliar de Qualea parviflora (Vochysiaceae) em três fitofisionomias do Estado Mato Grosso, Brasil RESUMOAs folhas são órgãos vegetativos que expressam uma variedade de características morfológicas e anatômicas influenciadas, principalmente, por fatores climáticos, edáficos e bióticos. O presente estudo objetivou levantar as características anatômicas das folhas de Qualea parviflora Mart. presente em três fitofisionomias: Savana Amazônica sobre afloramentos rochosos (SAR), Cerrado Rupestre de Transição (CRT) e Cerradão (CDA). Os cortes anatômicos foram realizados a mão livre e corados com azul de astra e fucsina básica 0,5%. Para a dissociação das epidermes, foi utilizado o método de Jeffrey modificado. As amostras da fitofisionomia de CRT apresentaram células epidérmicas comuns relativamente menores, maior quantidade de tricomas e mesofilo com poucos espaços intercelulares em relação as demais fitofisionomias. As amostras da fitofisionomia de SAR apresentaram maior índice estomático (IE: 21,05), maior quantidade de esclerênquima envolvendo o feixe vascular da nervura central (5 a 6 camadas), feixes vasculares secundários com células de paredes mais espessadas, com bainha que se estende até as células epidérmicas em ambas as faces. As amostras da fitofisionomia de CDA apresentaram caracteres de ambientes mesomórficos, como cutícula mais delgada. Pôde-se concluir que os espécimes que ocorrem em SAR e CRT apresentaram características xeromórficas que estão relacionados ao ambiente de ocorrência.

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Section: Methodsmentioning

confidence: 99%

Leaf anatomy of Qualea parviflora (Vochysiaceae) in three phytophysiognomies of the Mato Grosso State, Brazil

Ariano

Silva

2016

Acta Amaz.

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“…The middle third region of one leaf of each repetition was evaluated, and 10 fields were qualified. For studies related to the characterization of the stomata (average number per mm 2 , polar and equatorial diameter), the slides were photomicrographed with a 20x objective lens through the image capturer, coupled to the light microscope Leica  DMLB type, and the analyses were made through the program Anati Quant 2  UFV (Aguiar et al, 2007).…”

Section: Methodsmentioning

confidence: 99%

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

et al. 2017

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The aim of this study was to compare vitroplants Catasetum x apolloi grown under natural light and artificial light and different concentrations of potassium silicate, providing data on the anatomical differentiation that aids the acclimatization process of this species. Plants from in vitro seeding were used; 5 protocorms of approximately 0.5 cm were inoculated into vials with a capacity of 500 mL containing 100 mL of alternative culture medium plus potassium silicate (0.0, 0.5; 1.0 mL L -1 ), pH adjusted to 5.5 ± 0.5 and gelated with 4GL -1 agar before the autoclaving process. Cultures were maintained under natural light (TNE) and artificial light (TAE) for 90 days, and micromorphometric analysis was performed for polar and equatorial diameter, density and stomatal index, blade thickness in the central rib, and secondary veins. Applications in K 2 SiO 4 alternative medium provided the following: elongation of the hypodermis, thicker mesophyll, and more prominent midrib; elipptical guard cells; formation of epistomatal chamber; and lower stomatal density and stomatal with lower equatorial and polar diameters. The conditions that favored the acclimatization were lower light intensities and lower potassium silicate doses.Keywords: leaf anatomy, light, orchids, plants vitro. Anatomia e análise micromorfométrica foliar de Catasetum x apolloi Benelli & Grade com adição de silicato de potássio em diferentes fontes de luzResumo O objetivo desse trabalho foi comparar vitroplantas de Catasetum x apolloi cultivadas sob luz natural e luz artificial e diferentes concentrações de silicato de potássio, fornecendo dados sobre diferenciação anatômica que auxiliem no processo de aclimatação dessa espécie. Utilizou-se plantas provenientes da semeadura in vitro, 5 protocormos de aproximadamente 0,5 cm foram inoculados em frascos com capacidade para 500 mL contendo 100 mL de meio de cultura alternativo, acrescido de silicato de potássio (0,0; 0,5; 1,0 mL L -1 ), pH ajustado para 5,5 ±0,5 e gelificado com 4gL -1 de ágar antes do processo de autoclavagem. As culturas foram mantidas sob luz natural (TAA) e luz artificial (TAN) por 90 dias, e feitas análises micromorfométricas (diâmetro polar e equatorial, densidade e índice estomático, espessura do limbo na nervura central e nervuras secundárias). As aplicações de K 2 SiO 4 em meio alternativo, propiciaram: alongamento da hipoderme; mesofilo mais espesso e nervura central mais proeminente; células guardas elípticas; formação de câmaras supraestomáticas; menor densidade estomática e estômatos com menores diâmetros equatorial e polar. As condições que podem favorecer a aclimatação são menores intensidades de luz e menores doses de silicato de potássio.Palavras-chave: anatomia foliar, luminosidade, orquídeas, vitroplantas.

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“…Based on the photomicrographs and by means of the Anati Quanti 2  UFV program (Aguiar et al, 2007), the tissues from 144 transversal cuts of roots and 144 transversal cuts of leaves were measured, and the epidermal cells of 216 paradermal images were counted.…”

Section: Methodsmentioning

confidence: 99%

Death of pastures syndrome: tissue changes in Urochloa hybrida cv. Mulato II and Urochloa brizantha cv. Marandu

2017

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The quality of forage production is a prerequisite to raising livestock. Therefore, income losses in this activity, primarily cattle raising, can result in the impossibility of economic activity. Through the qualitative and quantitative anatomical study of Urochloa hybrida cv. Mulato II and U. brizantha cv. Marandu, we searched for descriptions and compared changes in the individual vegetative body from populations with death syndrome pastures (DPS). Specimens were collected at different physiological stages from farms in northern Mato Grosso. After collection, the individuals were fixed in FAA 50 and stored in 70% alcohol. Histological slides were prepared from the middle third of the sections of roots, rhizomes, and leaves, and the proportions and characteristics of tissues were evaluated in healthy, intermediate, and advanced stages of DPS. Changes were compared between cultivars. With the advancement of the syndrome, the following changes were observed: a more marked decrease in the length of roots in U. hybrida; disorganization of the cortical region of the roots and rhizome cultivars; fungal hyphae in roots and aerenchyma formation in U. hybrida; a decrease in sclerenchyma fiber proportions in roots and leaves; sclerification of the epidermis of U. brizantha rhizomes; and an increase in pericyclic fibers in U. hybrida. Furthermore, there was a decrease in the volume of epidermal cells of the abaxial face of the leaves of both cultivars, with a greater reduction in U. hybrida; a gradual decrease in thickness in the midrib of leaves similar to leaf mesophyll; conduction system obstructions; partial or total cell lysis in roots and rhizomes affected by the syndrome. Obstructions in sieve tube element and companion cells, and sometimes obstruction in xylem vessel elements. The evolution of DPS in cultivars was similar, but there were variations, arising probably from the physiological response to stress, such as aerenchyma formation in the root and increased pericycle in the rhizome of U. hybrida.Keywords: forage, plant anatomy, fungal hyphae. Síndrome da morte das pastagens: Alterações teciduais em Urochloa hybrida cv. Mulato II e Urochloa brizantha cv. Marandu ResumoA produção de forragem de qualidade é um pressuposto indispensável à atividade pecuária moderna. Dessa forma, perdas de rendimento nesta atividade preliminar à criação bovina, podem inviabilizar esta atividade econômica. Através de estudos anatômicos qualitativos e quantitativos em Urochloa hybrida cv. Mulato II e U. brizantha cv. Marandu buscou-se descrever e comparar as alterações do corpo vegetativo de indivíduos de populações que apresentam síndrome da morte das pastagens (SMP). Foram coletados indivíduos em estádios morfológicos distintos em propriedades rurais no norte de Mato Grosso, Amazônia Meridional. Após a coleta, os indivíduos foram fixados em FAA 50 e armazenados em álcool 70%. Lâminas histológicas foram confeccionadas a partir de secções do terço médio de raízes, rizomas e folhas, onde foram avaliadas as características e p...

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ANATI QUANTI: software de análises quantitativas para estudos em anatomia vegetal

Cited by 74 publications

References 14 publications

Leaf anatomy of Qualea parviflora (Vochysiaceae) in three phytophysiognomies of the Mato Grosso State, Brazil

Leaf anatomy of Qualea parviflora (Vochysiaceae) in three phytophysiognomies of the Mato Grosso State, Brazil

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

Death of pastures syndrome: tissue changes in Urochloa hybrida cv. Mulato II and Urochloa brizantha cv. Marandu

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