Anaphylactoid-Inflammation-Promoting Factor

Abstract

The experiments reported here indicate that, when exposed to insulin, viable lymphocytes rapidly released into the incubation medium a factor capable of increasing the dextran-induced anaphylactoid reaction, but having no effect on the inflammatory response evoked by 5-HT. This pro-inflammatory factor was shown to be elaborated by cell suspensions derived from lymph nodes of rats, rabbits, pigs or calves as well as from human tonsils. Thymus cells showed no such activity. The pro-inflammatory factor was termed as anaphylactoid-inflammation-promoting factor (AIPF). Its production depended upon the dose of insulin, and the time of exposure. AIPF was found to have an elution pattern in Sephadex G-100 gels similar to that of BSA (67,000 daltons). The activity was abolished by heat or incubation with DNase or α-chymotrypsin, but was not influenced by RNase. AIPF by itself did not induce increased vascular permeability, and proved to be distinct from the permeability factors present in the lysate of lymph node cells.

Insulin and the Dextran Reaction in the Rat

“…1 0 '4 10-3 10"2 Dextran concentration, g/ml ilar values were obtained when the experi ment was repeated in bilaterally adrenalectomised rats which were stated by Koltai et al [7] to be more sensitive and more consis tent than control animals when demonstrat ing insulin-induced sensitization of the dex tran anaphylactoid reaction.…”

“…However, in 1974, Koltai et al [8] found that the insulin-induced increase in the severity of the dextran anaphylactoid reaction was inhibited by several nucleic acid and protein synthesis inhibitors al though the hypoglycaemic action of the hor mone was not altered. Later, these authors [7] reported that the hormone initiated the de novo synthesis of a protein in lympho cytes which enhanced the interaction of rat mast cells with dextran, resulting in more mediator release and an increased anaphy lactoid reaction. They suggested that the enhancing effect of insulin may be due to the elaboration and release of this protein from lymphatic tissue.…”