Analyzing carotenoids of snow algae by Raman microspectroscopy and high-performance liquid chromatography

Osterrothová, Kateřina; Culka, Adam; Němečková, Kateřina; Kaftan, David; Nedbalová, Linda; Procházková, Lenka; Jehlička, Jan

doi:10.1016/j.saa.2019.01.013

Cited by 40 publications

(35 citation statements)

References 42 publications

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“…Comparative analysis of the ITS2 rRNA secondary structure showed that C. hindakii is closely genetically related to an uncultured environmental clone, ALBC6, from a glacier forefield in Switzerland (accession number JX435348, [51]) and to C. nivalis Gassan-B from snow in Japan (accession number LC012758, [52]), indicating that the closest relatives also thrive in cold habitats. However, by using solely light microscopy, the cysts of C. hindakii have probably been reported several times as those of C. nivalis (as “ Scotiella nivalis ”) in the Krkonoše [48,53,54,55,56] and High Tatra Mountains [49,57,58,59,60]. On the other hand, this is the first report about cryoflora from the Jeseníky Mountains.…”

Section: Discussionmentioning

confidence: 89%

Ecophysiology of Chloromonas hindakii sp. nov. (Chlorophyceae), Causing Orange Snow Blooms at Different Light Conditions

et al. 2019

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Slowly melting snowfields in mountain and polar regions are habitats of snow algae. Orange blooms were sampled in three European mountain ranges. The cysts within the blooms morphologically resembled those of Chloromonas nivalis (Chlorophyceae). Molecular and morphological traits of field and cultured material showed that they represent a new species, Chloromonas hindakii sp. nov. The performance of photosystem II was evaluated by fluorometry. For the first time for a snow alga, cyst stages collected in a wide altitudinal gradient and the laboratory strain were compared. The results showed that cysts were well adapted to medium and high irradiance. Cysts from high light conditions became photoinhibited at three times higher irradiances (600 µmol photons m−2 s−1) than those from low light conditions, or likewise compared to cultured flagellates. Therefore, the physiologic light preferences reflected the conditions in the original habitat. A high content of polyunsaturated fatty acids (about 60% of total lipids) and the accumulation of the carotenoid astaxanthin was observed. They are regarded as adaptations to cope with extreme environmental conditions of snow that include low temperatures, freeze-thaw cycles, and variable light intensity. The intraspecific ability of adaptation of the photosynthetic apparatus to different irradiance regimes seems to be advantageous for thriving in different snow habitats.

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Section: Discussionmentioning

confidence: 89%

Ecophysiology of Chloromonas hindakii sp. nov. (Chlorophyceae), Causing Orange Snow Blooms at Different Light Conditions

et al. 2019

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“…[16,19,25] Raman spectra of carotenoid pigments lutein, zeaxanthin, peridinin, and astaxanthin are characteristic for cyanobacteria or algae. [45,46] Long-chain 50-C carotenoids such as bacterioruberin and its derivatives are characteristic for halophiles. In this case, the position of the ν 1 (C═C) of the polyene chain fall to the area 1,506-1,509 cm −1 .…”

Section: Sample G17-07mentioning

confidence: 99%

Raman spectroscopic screening of cyanobacterial chasmoliths from crystalline gypsum—The Messinian crisis sediments from Southern Sicily

Jehlička

Culka

Mareš

2019

J Raman Spectroscopy

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This study shows the first results on Raman spectroscopic investigations of pigments from cyanobacterial endolithic colonisations in gypsum of Messinian age (Miocene). Gypsum from outcrops of sedimentary series close to Eraclea Minoa (Sothern Sicily) is sometimes inhabited by endoliths. Raman spectroscopic investigations allow to specify type of colonisation through identification of their pigments. Coccoid cyanobacteria (Chroococcidiopsis sp., Gloeocapsopsis pleurocapsoides, Gloeocapsa compacta, and Synechococcus sciophilus), filamentous heterocytous Nostoc sp., and filamentous bundle‐forming Symplocastrum cf. aurantiacum and Microcoleus sp. were found to inhabit different parts of the gypsum samples. Carotenoids (as assumed frequently β‐carotene) are produced in all the samples studied (optimised detection using 514‐nm excitation), scytonemin in the samples colonised by G. pleurocapsoides (G1, G2, and G6), and phycobiliproteins in the sample G3 (those detected using 785‐nm excitation). This study is the first initiative to collect better knowledge on detecting biomarker traces in the frame of Messinian‐age gypsum sequence of relevance for old Martian environments.

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“…It is now well established that the wavenumber of ν 1 (C═C) Raman feature of carotenoids decreases as the number of double bonds in polyene chain increases 36 . This general observation was reported by Kuzmany 54 and discussed in the case of carotenes of vegetal or microbial origin, for example, by de Oliveira et al 37b or Osterrothová et al 55 …”

Section: Resultsmentioning

confidence: 55%

Fast screening of carotenoids of gypsum endoliths using portable Raman spectrometer (Messinian gypsum, Sicily)

Němečková

Jehlička

Culka

2020

J Raman Spectroscopy

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We tested the potential of a portable Raman spectrometer—RaPort (G) by EnSpectr with a 532‐nm laser for use in field detection of biomarkers of endolithic colonization in gypsum. This study extends our previous research on gypsum endolithic colonies using laboratory Raman microspectrometer (514.5‐ and 785‐nm lasers). In the current study, variously coloured layers were found and screened separately on the outcrop under natural conditions. In the recorded Raman spectra, stretching vibrations of carotenoids were observed—ν1 (C═C) around 1,513 cm−1, and ν2 (C—C) around 1,154 cm−1, as well as the feature at around 1,005 cm−1. The exact identification of different carotenoids by portable Raman spectrometer is limited. Nevertheless, the portable Raman spectrometer allowed fast and in situ detection of carotenoids of variously pigmented gypsum endolithic zones. Application of miniature Raman instruments is important in the context of forthcoming astrobiological missions to Mars (Exomars and Mars 2020).

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Analyzing carotenoids of snow algae by Raman microspectroscopy and high-performance liquid chromatography

Cited by 40 publications

References 42 publications

Ecophysiology of Chloromonas hindakii sp. nov. (Chlorophyceae), Causing Orange Snow Blooms at Different Light Conditions

Ecophysiology of Chloromonas hindakii sp. nov. (Chlorophyceae), Causing Orange Snow Blooms at Different Light Conditions

Raman spectroscopic screening of cyanobacterial chasmoliths from crystalline gypsum—The Messinian crisis sediments from Southern Sicily

Fast screening of carotenoids of gypsum endoliths using portable Raman spectrometer (Messinian gypsum, Sicily)

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