Analyzing bean extracts using time-dependent SDS trapping to quantify the kinetic stability of phaseolin proteins

Thibeault, Jane; Church, Jennifer; Ortiz-Perez, Brian; Addo, Samuel; Hill, Shakeema; Khalil, Areeg; Young, M A; Xia, Ke; Colón, Wilfredo

doi:10.1016/j.bbrc.2017.07.166

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…7,8,[13][14][15][16][17] AtrbohE is involved in modulating programmed cell death, 18 and AtrbohI regulates drought stress response in seeds and roots. 19 Both AtrbohH and AtrbohI are key regulators of pollen tube tip growth and polar root hair growth. 20,21 NOXs also play pivotal roles in numerous cellular processes regulated by phtohormones.…”

Section: Reactive Oxygen Species (Ros) Such As Superoxide Anion (Omentioning

confidence: 99%

NADPH oxidases, essential players of hormone signalings in plant development and response to stresses

Sun

Zhao

Hao

2019

Plant Signaling & Behavior

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Plasma membrane NADPH oxidases (NOXs), also named respiratory burst oxidase homologues (Rbohs), are critical generators of reactive oxygen species (ROS), which as signal molecules regulate growth and development, and adaptation to various biotic and abiotic stresses in plants. NOXs-dependent ROS production is frequently induced by diverse phytohormones. The ROS commonly function downstream of, and interplay with hormone signalings, coordinately modulating plant development and stress tolerance. In this review, we summarize recent advances on the roles and molecular mechanisms of Rbohs in mediating signalings of multiple hormones including auxin, gibberellins, abscisic acid, ethylene and brassinosteroids in plants.

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Section: Reactive Oxygen Species (Ros) Such As Superoxide Anion (Omentioning

confidence: 99%

NADPH oxidases, essential players of hormone signalings in plant development and response to stresses

Sun

Zhao

Hao

2019

Plant Signaling & Behavior

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“…However in common bean seed case, the presence of phaseolin represents about 50% of total amount of protein [25]. So, in germinative stage this storage glycoproteins have the function of provide amino acids to seed [26]. The phaseolin protein show low enzymatic proteolysis and digestibility, but under certain conditions can be hydrolyzed, becoming small peptides with antioxidant and iron chelating potential [27,28].…”

Section: Introductionmentioning

confidence: 99%

Phaseolin Induction on Common-Bean Cultivars and Biological Control of Colletotrichum lindemuthianum 89 Race by Baccharis trimera (Less.) Dc.

Pozza

Pandini

Hein

et al. 2021

Braz. arch. biol. technol.

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The objective of this work was to evaluate the fungitoxic effect of the aqueous extracts of Baccharis trimera on the mycelial growth of Colletotrichum lindemuthianum 89 race, as well as its effect on the accumulation of phaseolin in hypocotyls of different cultivars and common bean varieties. It was obtained 20% aqueous extract from plants collected in municipalities of the Western Region of Paraná. Blocks containing C. lindemuthianum mycelium were transferred to Petri dishes containing medium with the different extracts and incubated at 25 °C. The colonies diameter was measured until the 12th day. Effects of aqueous extracts on phaseolin production was evaluated in hypocotyls of Carioca, Cnpf 8104, Soberana, Tibatã, Uirapurú cultivars, as well as Rosinha and Vermelho varieties. Each one cultivar and variety hypocotyl was transferred separately to test tubes containing 500 μL of 20% aqueous extracts. Sterile water, Bion®, and UV was used as controls. The phaseolin production was measured in spectrophotometer [280 nm]. Results of the evaluation of the antifungal activity of aqueous extracts of Baccharis sp. specimens collected indicate that approximately 50% of the samples presented capacity to reduce between 74 and 92% of C. lindemuthianum growth. Cultivar Tibatã and Vermelho variety showed greater sensitivity over the applied HIGHLIGHTS  Baccharis trimera aqueous extracts can inhibit Colletotrichum lindemuthianun 89 race growth.  C. lindemuthianum filtrate can be used as a phaseolin inductor on common bean.  There is no evidence of phaseolin induction through application of aqueous extracts of B. trimera in common bean hypocotyls.

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Sarkosyl: A milder detergent than SDS for identifying proteins with moderately high hyperstability using gel electrophoresis

Thibeault

Patrick

Martin

et al. 2019

Analytical Biochemistry

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Analyzing bean extracts using time-dependent SDS trapping to quantify the kinetic stability of phaseolin proteins

Cited by 4 publications

References 15 publications

NADPH oxidases, essential players of hormone signalings in plant development and response to stresses

NADPH oxidases, essential players of hormone signalings in plant development and response to stresses

Phaseolin Induction on Common-Bean Cultivars and Biological Control of Colletotrichum lindemuthianum 89 Race by Baccharis trimera (Less.) Dc.

Sarkosyl: A milder detergent than SDS for identifying proteins with moderately high hyperstability using gel electrophoresis

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