Analytically derived material properties of multilaminated extracellular matrix devices using the ball-burst test

Hybrid nanofibrous scaffolds from electrospinning of a synthetic biodegradable elastomer and urinary bladder matrix

“…5). The maximum stresses and maximum strains of the biohybrid scaffolds were similar or greater than previously reported values for lyophilized sheets of UBM (0.3-0.4 MPa and 47-67% strain, respectively) [19,28]. It is believed that addition of larger amounts of UBM may cause some disruptions in the PEUU physical cross-linking or hydrogen bonding (in addition to the mass reduction effect) that could have lead to the reductions in tensile strengths and breaking strains.…”

Clinically relevant mechanical testing of hernia graft constructs

“…Ball-burst tests (Whitson et al, 1998;Freytes et al, 2005;Deeken et al, 2012;Eliason et al, 2011;Sahoo et al, 2014), planar-biaxial tests (Rohrnbauer and Mazza, 2014;Sun et al, 2005;Lyons et al, 2014;Sahoo et al, 2014), and also inflation tests (Rohrnbauer and Mazza, 2013) can each partly replicate this complex physiologic loading mechanism through their respective modes of loading, and offer different considerations as options for testing hernia grafts. This study used ball-burst and planar-biaxial tests to test hernia grafts as sutured constructs to model the manner in which the grafts are placed and loaded clinically for ventral hernia repair.…”

“…Further, the test modes and strain analysis methods are inherently different. Ball-burst tests constrain the graft edges and apply an orthogonal load to the central region of the graft (Freytes et al, 2005). Any graft anisotropy is obscured due to the geometric constraints.…”

“…Data analysis (details in Appendix A). Dilatational strain: Using the previously described analytical solution for ball-burst testing (Freytes et al, 2005;Deeken et al, 2011aDeeken et al, , 2011b, the geometry of the test setup ( Fig. 3B) and the axial ball displacement (ℓ, mm) were used to estimate dilatational strain (DS, %), defined as the percent change in graft area while the ball traversed the construct:…”

Reprint of: Extracellular matrix as a biological scaffold material: Structure and function

“…the tissue for which it was originally intended to function as a biological reinforcement scaffold) and imparts isotropy to natively anisotropic materials. Lamination via vacuum pressing of ECM scaffolds also reduces the extensibility, and changes the ultrastructural morphology of the resulting construct [22,64]. Vacuum pressing is an effective method for constructing a variety of 3-D shapes of ECM scaffold materials (Fig.…”