Reprint of: Extracellular matrix as a biological scaffold material: Structure and function

Badylak, Stephen F.; Freytes, Donald O.; Gilbert, Thomas W.

doi:10.1016/j.actbio.2015.07.016

Cited by 449 publications

(91 citation statements)

References 113 publications

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“…To date, clinical application with decellularized ECM has included acellular administration, mainly in the form of ECM sheets as surgical patches [90, 91], and recently the initiation of a clinical trial with an injectable cardiac ECM derived hydrogel (clinicaltrials.gov identifier NCT02305602). The acellular route is undoubtedly easier in terms of translation and has the potential to modulate the fate of endogenous progenitor cells.…”

Section: Current Challenges and Future Opportunitiesmentioning

confidence: 99%

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Agmon

Christman

2016

Current Opinion in Solid State and Materials Science

150

104

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Decellularized tissues have become a common regenerative medicine platform with multiple materials being researched in academic laboratories, tested in animal studies, and used clinically. Ideally, when a tissue is decellularized the native cell niche is maintained with many of the structural and biochemical cues that naturally interact with the cells of that particular tissue. This makes decellularized tissue materials an excellent platform for providing cells with the signals needed to initiate and maintain differentiation into tissue-specific lineages. The extracellular matrix (ECM) that remains after the decellularization process contains the components of a tissue specific microenvironment that is not possible to create synthetically. The ECM of each tissue has a different composition and structure and therefore has unique properties and potential for affecting cell behavior. This review describes the common methods for preparing decellularized tissue materials and the effects that decellularized materials from different tissues have on cell phenotype.

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Section: Current Challenges and Future Opportunitiesmentioning

confidence: 99%

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Agmon

Christman

2016

Current Opinion in Solid State and Materials Science

150

104

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“…9 For example, the release of cytokines and growth factors, with basic fibroblast growth factor (bFGF) and transforming growth factor (TGF)- μ 1 being the most abundant, 29 may play important roles in promoting cell proliferation, matrix production, and angiogenesis. These bioactive factors may account for at least some of the positive effects observed with MatriStem in the current study.…”

Section: Commentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extracellular matrix regenerative graft attenuates the negative impact of polypropylene prolapse mesh on vagina in rhesus macaque

Liang

Knight

Barone

et al. 2017

American Journal of Obstetrics and Gynecology

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BACKGROUND The use of wide pore lightweight polypropylene mesh to improve anatomical outcomes in the surgical repair of prolapse has been hampered by mesh complications. One of the prototype prolapse meshes has been found to negatively impact the vagina by inducing a decrease in smooth muscle volume and contractility and the degradation of key structural proteins (collagen and elastin), resulting in vaginal degeneration. Recently, bioscaffolds derived from extracellular matrix have been used to mediate tissue regeneration and have been widely adopted in tissue engineering applications. OBJECTIVE Here we aimed to: (1) define whether augmentation of a polypropylene prolapse mesh with an extracellular matrix regenerative graft in a primate sacrocolpopexy model could mitigate the degenerative changes; and (2) determine the impact of the extracellular matrix graft on vagina when implanted alone. STUDY DESIGN A polypropylene-extracellular matrix composite graft (n = 9) and a 6-layered extracellular matrix graft alone (n = 8) were implanted in 17 middle-aged parous rhesus macaques via sacrocolpopexy and compared to historical data obtained from sham (n = 12) and the polypropylene mesh (n = 12) implanted by the same method. Vaginal function was measured in passive (ball-burst test) and active (smooth muscle contractility) mechanical tests. Vaginal histomorphologic/ biochemical assessments included hematoxylin-eosin and trichrome staining, immunofluorescent labeling of α-smooth muscle actin and apoptotic cells, measurement of total collagen, collagen subtypes (ratio III/ I), mature elastin, and sulfated glycosaminoglycans. Statistical analyses included 1-way analysis of variance, Kruskal-Wallis, and appropriate posthoc tests. RESULTS The host inflammatory response in the composite mesh-implanted vagina was reduced compared to that following implantation with the polypropylene mesh alone. The increase in apoptotic cells observed with the polypropylene mesh was blunted in the composite (overall P < .001). Passive mechanical testing showed inferior parameters for both polypropylene mesh alone and the composite compared to sham whereas the contractility and thickness of smooth muscle layer in the composite were improved with a value similar to sham, which was distinct from the decreases observed with polypropylene mesh alone. Biochemically, the composite had similar mature elastin content, sulfated glycosaminoglycan content, and collagen subtype III/I ratio but lower total collagen content when compared to sham (P = .011). Multilayered extracellular matrix graft alone showed overall comparable values to sham in aspects of the biomechanical, histomorphologic, or biochemical end-points of the vagina. The increased collagen subtype ratio III/I with the extracellular matrix graft alone (P = .033 compared to sham) is consistent with an ongoing active remodeling response. CONCLUSION Mesh augmentation with a regenerative extracellular matrix graft attenuated the negative impact of polypropylene mesh on the vagina. Applicat...

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“…9 For example, the release of cytokines and growth factors, with basic fibroblast growth factor (bFGF) and transforming growth factor (TGF)-μ1 being the most abundant, 29 may play important roles in promoting cell proliferation, matrix production, and angiogenesis. These bioactive factors may account for at least some of the positive effects observed with MatriStem in the current study.…”

Section: Commentmentioning

confidence: 99%

“…It has been shown that these grafts, when placed in the appropriate micro-and loading environments, can promote site-specific functional tissue remodeling by limiting destructive inflammatory responses, recruiting requisite cell populations including progenitor cells, and supporting cell attachment, proliferation, and matrix production. 9,10 We chose to use MatriStem Surgical Matrix RS (MatriStem RS, ACell Inc, Columbia, MD), a noncross-linked degradable acellular porcine urinary bladder matrix, because it facilitates reconstructive remodeling of smooth muscle and connective tissue as shown in its application in cardiac defect repair, esophagus reinforcement, and chronic wound repairs. [11][12][13][14] It is important to distinguish regenerative matrices such as MatriStem (noncross-linked) from biologic materials that have been chemically cross-linked (eg, Pelvicol, Bard, Murray Hill, NJ) to provide mechanical support similar to polypropylene mesh.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Matrix Regenerative Graft Attenuates the Negative Impact of Polypropylene Prolapse Mesh on Vagina in Rhesus Macaque

Liang

Knight

Barone

et al. 2017

Obstet Gynecol Surv

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BACKGROUND-The use of wide pore lightweight polypropylene mesh to improve anatomical outcomes in the surgical repair of prolapse has been hampered by mesh complications. One of the prototype prolapse meshes has been found to negatively impact the vagina by inducing a decrease in smooth muscle volume and contractility and the degradation of key structural proteins (collagen and elastin), resulting in vaginal degeneration. Recently, bioscaffolds derived from extracellular matrix have been used to mediate tissue regeneration and have been widely adopted in tissue engineering applications.

show abstract

Reprint of: Extracellular matrix as a biological scaffold material: Structure and function

Cited by 449 publications

References 113 publications

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Extracellular matrix regenerative graft attenuates the negative impact of polypropylene prolapse mesh on vagina in rhesus macaque

Extracellular Matrix Regenerative Graft Attenuates the Negative Impact of Polypropylene Prolapse Mesh on Vagina in Rhesus Macaque

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