Analytical validation and clinical evaluation of a commercially available high-sensitivity immunoassay for the measurement of troponin I in humans for use in dogs

Winter, Randolph L.; Saunders, Ashley B.; Gordon, Sonya G.; Miller, Matthew W.; Sykes, K.T.; Suchodolski, Jan S.; Steiner, Jörg M.

doi:10.1016/j.jvc.2014.03.002

Cited by 40 publications

(62 citation statements)

References 37 publications

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“…Littermate #3 had the highest serum cTnI concentration (0.847 ng/mL), suggesting that analysis of serum cTnI concentrations should be considered in identifying acute myocarditis in dogs with Chagas disease. A cause for the detectable serum cTnI concentration in the dam and littermates (#1 and #2) was not identified, and results may have been normal based on concentrations that were within the range reported for healthy dogs …”

mentioning

confidence: 97%

Diagnostic Features of Acute Chagas Myocarditis with Sudden Death in a Family of Boxer Dogs

Vitt

Saunders

O'Brien

et al. 2016

Veterinary Internal Medicne

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mentioning

confidence: 97%

Diagnostic Features of Acute Chagas Myocarditis with Sudden Death in a Family of Boxer Dogs

Vitt

Saunders

O'Brien

et al. 2016

Veterinary Internal Medicne

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“…Additionally these cTn kits have been validated for use in the cow [27] , calf [28,29] and lambs [30] . Recently, an high-sensitivity immunoassay (direct chemiluminometric method) has been described in clinically affected dogs [12,13] . But these …”

Section: Discussionmentioning

confidence: 99%

“…The kits and assays that used to detect the levels of the troponins in humans have also been used in dogs [9][10][11][12][13] . In recent publications, the concentration of cTnI has been investigated in different canine cardiovascular diseases, including congenital cardiac diseases [12,14,15] , acquired cardiac heart diseases such as arrhythmogenic right ventricular dysplasia [16] , pericardial effusion [15,17,18] , cardiac contusion [19] , experimental infarction [20] , cardiovascular injury, pacing, cardiotoxic drugs with positive and inotropic effects [21] neoplasia [22] dogs with dilated cardiomyopathy [23] and parvoviral enteritis [24] . A previous study also showed to use cTnl to evaluate the efficacy of antiarrhythmia treatment in dogs [25] .…”

Section: The Rapid Analyses Of Cardiac Troponins In Dogs With Dilatedmentioning

confidence: 99%

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Dilate Kardiyomiyopati, Distemper yada Parvoviral İnfeksiyonlu Köpeklerde Kardiyak Troponinin Hızlı Analizi

Güneş¹,

Uyanik²,

Eren³

et al. 2014

Kafkas Univ Vet Fak Derg

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In this study, concentrations of cardiac troponins (cTnI and cTnT) and using a cassette kit which is a qualitative assay were used to investigate the diagnosis of myocardial damages due to different diseases in dogs. Study groups were composed of dogs with distemper infection (DI) (Group I, n=8), parvoviral infection (PVI) (Group II, n=18) and dilated cardiomyopathy (DCM) (Group III, n=18). Healthy dogs were included for each group as control. Levels of troponins (cTnI, cTnT) were determined by commercially available ELISA kits and cassette kits were used for qualitative assays. The mean concentrations of cTnI in Groups I, II and III were 2.26±0.41 ng/ml, 2.41±0.98 ng/ml and 5.076±1.32 ng/ml, respectively. Positive cTnI and cTnT expressions were determined in groups as 5/8 (62.5%) and 4/8 (50%) in group I; 5/18 (27.7%) and 5/18 (27.7%) in group II and 13/18 (72.2%) and 6/18 (33.3%) in group III, respectively. In healthy control dogs, cTnI and cTnT were determined to be negative. The cTnI concentrations were more reliable cardiac marker in dogs with cardiomyopathy. Moreover, cTnI and cTnT casette kits used in this study may be valuable diagnostic tools to diagnose the myocardial damage in dogs with DI, PVI and DCM for the small animal practioners. Özet Bu çalışmada, köpeklerdeki farklı hastalıklara bağlı miyokardial dejenerasyonların teşhisinde kardiyak troponin (cTnI, cTnT) konsantrasyonları ve kalitatif analiz yapan kaset kitlerinin kullanımı araştırıldı. Çalışma grupları distemper enfeksiyonlu (DE) (Group I, n=8), parvoviral enfeksiyonlu (PVE) (Group II, n=18) ve dilate kardiyomiyopatili (Group III, n=18) köpeklerden oluşturuldu. Her bir grubun kontrolü için sağlıklı köpekler çalışmaya dahil edildi. Ticari ELISA kitleri ile troponin (cTnI, cTnT) düzeyleri belirlendi ve kalitatif analizler için kaset kitler kullanıldı. Grup I, II ve III'de ortalama cTnI düzeyleri sırasıyla 2.26±0.41 ng/ml, 2.41±0.98 ng/ml ve 5.076±1.32 ng/ml idi. Pozitif cTnI ve cTnT varlığı da sırasıyla grup I'de 5/8 (62.5%) ve 4/8 (50%); grup II'de 5/18 (27.7%) ve 5/18 (27.7%), ve grup III'te 13/18 (72.2%) ve 6/18 (33.3%) olarak belirlendi. Sağlıklı kontrol köpeklerinde cTnI ve cTnT testlerinin negatif olduğu belirlendi. Kardiyomiyopatili köpeklerde kalp biyobelirteçleri daha güvenilirdi. Ayrıca cTnI ve cTnT kaset kitlerinin küçük hayvan pratisyenleri için; DE, PVE ve kardiyomiyopatili köpeklerde miyokarditisi belirlemek için faydalı bir teşhis aracı olabileceği belirlendi.

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“…23,36 Assays were carried out in a batched analysis and in duplicate to minimise possible measurement variation. Samples were transported for analysis on dry ice.…”

Section: Laboratory Measurementsmentioning

confidence: 99%

NT‐proBNPand cardiac troponin I concentrations in dogs with tick paralysis caused byIxodes holocyclus

Nicolson

McGrath

Webster³

et al. 2016

Aust Veterinary J

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This study did not detect significant cardiac involvement in dogs with tick paralysis induced by I. holocyclus. Evidence for reduced preload in dogs with tick paralysis was provided by lower NT-proBNP concentrations compared with control dogs. Severe hypoxaemia may not be a significant component of the clinical picture in many of the dogs presenting with tick paralysis. Dogs with severe hypoxaemia may have loss of cardiomyocyte integrity, reflected by elevated cTnI concentrations.

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Analytical validation and clinical evaluation of a commercially available high-sensitivity immunoassay for the measurement of troponin I in humans for use in dogs

Cited by 40 publications

References 37 publications

Diagnostic Features of Acute Chagas Myocarditis with Sudden Death in a Family of Boxer Dogs

Diagnostic Features of Acute Chagas Myocarditis with Sudden Death in a Family of Boxer Dogs

Dilate Kardiyomiyopati, Distemper yada Parvoviral İnfeksiyonlu Köpeklerde Kardiyak Troponinin Hızlı Analizi

NT‐proBNPand cardiac troponin I concentrations in dogs with tick paralysis caused byIxodes holocyclus

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