Analytical techniques for griseofulvin

Abstract

Griseofulvin is an antifungal antibiotic used to treat various pathogenic mycotic diseases of animals and plants. Various analytical techniques for the determination of griseofulvin in pure form, in different chemical mixtures, in biological¯uids and in fermentation samples are described in this review. The present communication reviews the use of spectrophotometry, spectro¯uorimetry, paper chromatography, thin layer chromatography, gas chromatography, high performance liquid chromatography and microbiological assay for the estimation of griseofulvin.

CaCO3-based carriers with prolonged release properties for antifungal drug delivery to hair follicles

“…A quantitative assessment of the Gf amount immobilized into the CaCO 3 microcarriers was carried out by UV-visible spectrophotometry, which is known to be routinely utilized for its determination in various media. 67,68 Preliminary comparison of the spectrometric and HPLC data was done as previously described. 69 In order to evaluate the loading capacity of vaterite particles with Gf, the solutions collected after Gf-loaded microcarriers’ dissolution were measured.…”

Transformations of Griseofulvin in Strong Acidic Conditions – Crystal Structures of 2′-Demethylgriseofulvin and Dimerized Griseofulvin

“…[(2S,6'R)-7-chloro-2',4,6-trimethoxy-6'-methyl-3H,4'H-spiro[1-benzofuran-2,1'-cyclohex [2]ene]-3,4'-dione] (I) is a classical antifungal antibiotic used clinically for the treatement of dermatomycoses. It was also identified as an inhibitor of centrosomal clustering (important cellular event that enables biopolar mitosis for cancer cell lines harboring supernumerary centrosomes).…”

Detection of griseofulvin in a marine strain of Penicillium waksmanii by ion trap mass spectrometry

“…extract with a high degree of certainty, which could not be obtained with classical methods such as TLC or HPLC-UV (Fischer and Riegelman, 1966;Zia et al, 1980;Frisvad and Filtenborg, 1989;Venkatadasu et al, 2000). Our study is a typical application of the use of ESI/MS providing "mass profiles" of fungal extracts (Smedsgaard and Frisvad, 1996).…”