Analytical pyrolysis-based study on intra-skeletal organic matrices from Mediterranean corals

Adamiano, Alessio; Goffredo, Stefano; Dubinsky, Zvy; Levy, Oren; Fermani, Simona; Fabbri, Daniele; Falini, Giuseppe

doi:10.1007/s00216-014-7995-1

Cited by 19 publications

(19 citation statements)

References 52 publications

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“…Secondly, persistent organic material in the denuded skeleton likely includes the organic matrix, for which lipids have been shown to play a significant role in forming ( Marin & Luquet, 2008 ; Adamiano et al, 2014 ). Changes in production rates of the organic matrix has been attributed to growth and skeletal deposition ( Stolarski, 2003 ).…”

Section: Discussionmentioning

confidence: 99%

A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences

Conlan

Rocker

Francis

2017

PeerJ

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Lipids are involved in a host of biochemical and physiological processes in corals. Therefore, changes in lipid composition reflect changes in the ecology, nutrition, and health of corals. As such, accurate lipid extraction, quantification, and identification is critical to obtain comprehensive insight into a coral’s condition. However, discrepancies exist in sample preparation methodology globally, and it is currently unknown whether these techniques generate analogous results. This study compared the two most common sample preparation techniques for lipid analysis in corals: (1) tissue isolation by air-spraying and (2) crushing the coral in toto. Samples derived from each preparation technique were subsequently analysed to quantify lipids and their constituent classes and fatty acids in four common, scleractinian coral species representing three distinct morphotypes (Acropora millepora, Montipora crassotuberculata, Porites cylindrica, and Pocillopora damicornis). Results revealed substantial amounts of organic material, including lipids, retained in the skeletons of all species following air-spraying, causing a marked underestimation of total lipid concentration using this method. Moreover, lipid class and fatty acid compositions between the denuded skeleton and sprayed tissue were substantially different. In particular, the majority of the total triacylglycerol and total fatty acid concentrations were retained in the skeleton (55–69% and 56–64%, respectively). As such, the isolated, sprayed tissue cannot serve as a reliable proxy for lipid quantification or identification in the coral holobiont. The in toto crushing method is therefore recommended for coral sample preparation prior to lipid analysis to capture the lipid profile of the entire holobiont, permitting accurate diagnoses of coral condition.

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Section: Discussionmentioning

confidence: 99%

A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences

Conlan

Rocker

Francis

2017

PeerJ

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“…As a source of SOM, symbiotic and a‐symbiotic coral species were used, which allowed the features related to the daytime photosynthetic activities of the organism to be highlighted. The content of SOM extracted from B. europaea was higher than in L. pruvoti , whereas their amino acid compositions were almost identical.…”

Section: Discussionmentioning

confidence: 91%

In Vitro Coral Biomineralization under Relevant Aragonite Supersaturation Conditions

Džakula

Fermani

Dubinsky

et al. 2019

Chemistry A European J

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The biomineralization of corals occurs under conditions of high and low supersaturation with respect to aragonite, which corresponds to day‐ or night‐time periods of their growth, respectively. Here, in vitro precipitation of aragonite in artificial seawater was investigated at a high supersaturation, allowing spontaneous nucleation and growth, as well as at low supersaturation conditions, which allowed only the crystal growth on the deliberately introduced aragonite seeds. In either chemical systems, soluble organic matrix (SOM) extracted from Balanophyllia europaea (light sensitive) or Leptopsammia pruvoti (light insensitive) was added. The analyses of the kinetic and thermodynamic data of aragonite precipitation and microscopic observations showed that, at high supersaturation, the SOMs increased the induction time, did not affect the growth rate and were incorporated within aggregates of nanoparticles. At low supersaturation, the SOMs affected the aggregation of overgrowing crystalline units and did not substantially change the growth rate. On the basis of the obtained results we can infer that at high supersaturation conditions the formation of nanoparticles, which is typically observed in the skeleton's early mineralization zone may occur, whereas at low supersaturation the overgrowth on prismatic seeds observed in the skeleton fiber zone is a predominant process. In conclusion, this research brings insight on coral skeletogenesis bridging physicochemical (supersaturation) and biological (role of SOM) models of coral biomineralization and provides a source of inspiration for the precipitation of composite materials under different conditions of supersaturation.

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“…content was evaluated according to the weight loss observed between 550 and 950°C as already described in the literature for carbonated HA [35,36].…”

Section: -mentioning

confidence: 99%

On the use of superparamagnetic hydroxyapatite nanoparticles as an agent for magnetic and nuclear in vivo imaging

et al. 2018

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The ability of iron-doped hydroxyapatite nanoaprticles (FeHA) to work in vivo as imaging agents for magnetic resonance (MR) and nuclear imaging is demonstrated. FeHA applied an higher MR contrast in the liver, spleen and kidneys of mice with respect to Endorem®. The successful radiolabeling of FeHA allowed for scintigraphy/X-ray and ex vivo biodistribution studies, confirming MR results and envisioning FeHA application for dual-imaging.

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Analytical pyrolysis-based study on intra-skeletal organic matrices from Mediterranean corals

Cited by 19 publications

References 52 publications

A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences

A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences

In Vitro Coral Biomineralization under Relevant Aragonite Supersaturation Conditions

On the use of superparamagnetic hydroxyapatite nanoparticles as an agent for magnetic and nuclear in vivo imaging

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