Analytical profiling and stability evaluation of liposomal drug delivery systems: A rapid UHPLC-CAD-based approach for phospholipids in research and quality control

Weber, Florian; Rahnfeld, Lisa; Luciani, Paola

doi:10.1016/j.talanta.2020.121320

Cited by 20 publications

(8 citation statements)

References 41 publications

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“…After liposome preparation, the lipid content of the different formulations was analyzed by a UHPLC‐CAD‐based approach (see Supporting Information) using a method previously developed in our group. [ 82 ]…”

Section: Methodsmentioning

confidence: 99%

“…After liposome preparation, the lipid content of the different formulations was analyzed by a UHPLC-CAD-based approach (see Supporting Information) using a method previously developed in our group. [82] Size Evaluation and Zeta Potential: The hydrodynamic diameter and the polydispersity index (PDI) of the liposomes were determined by a Litesizer 500 (Anton Paar, Graz, Austria) equipped with a 175 backscatter angle detector and a semiconductor laser with λ ¼ 658 nm. Briefly, the liposome formulations were diluted to a concentration of 100 μM, and 1 mL of the diluted dispersion was transferred into disposable semimicro cuvettes.…”

Section: Methodsmentioning

confidence: 99%

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Beyond Trial and Error: A Systematic Development of Liposomes Targeting Primary Macrophages

Weber

Ivan

Proulx

et al. 2021

Advanced NanoBiomed Research

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Monocytes/macrophages are phagocytic innate immune cells playing a pivotal role in tissue homeostasis, inflammation, and antitumor immunity in a microenvironment‐dependent manner. By expressing pattern recognition and scavenger receptors on their surface, macrophages selectively take up pathogens, cellular debris, and often—undesirably—drug delivery systems. On the other hand, the propensity of phagocytic cells to internalize particulate drug carriers is used to load them with a cargo of choice, turning the monocytes/macrophages into a diagnostic or therapeutic Trojan horse. Identifying the ideal physicochemical properties of particulate carriers such as liposomes to achieve the most efficient macrophage‐mediated drug delivery has been object of extensive research in the past, but the studies reported so far rely solely on trial‐and‐error approaches. Herein, a design of experiment (DoE) strategy to identify the optimal liposomal formulation is proposed, fully characterized in terms of size, surface charge, and membrane fluidity, to maximize macrophage targeting. The findings are validated using mouse bone marrow‐derived macrophages, a primary preparation modeling in vivo monocyte‐derived macrophages, thus confirming the robustness and versatility of the systematic and iterative approach and suggesting the promising potential of the DoE approach for the design of cell‐targeting delivery systems.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Beyond Trial and Error: A Systematic Development of Liposomes Targeting Primary Macrophages

Weber

Ivan

Proulx

et al. 2021

Advanced NanoBiomed Research

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“…epoxy-13-cis retinoic acid, tretinoin, and others unknown. Some impurities were observed with the related substance test of the liposomal formulation 30 . The results were compared with the USP monograph limits.…”

Section: Related Substances (Detection Of Impurity)mentioning

confidence: 99%

Method Development and Characterization of Liposomal Formulation of Isotretinoin

Ahmad¹,

Kumar²,

Singh³

et al. 2021

Borneo J Pharm

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This study aims to develop a liposomal drug delivery system of isotretinoin, an acne drug-using spray drying, as a cost-effective and time-effective technique. The liposomal formulation was prepared by using spray drying; three different strategies were adopted: suspension spray drying (SSD), thin-film hydration and spray drying (TFHSD), and emulsion spray drying (ESD). Isotretinoin was 99% bound with lipid, so lipids hydrogenated soy phosphatidylcholine (HSPC), distearoyl phosphatidylglycerol (DSPG), and cholesterol were selected for the formulation development. The HSPC, DSPG, cholesterol, and isotretinoin were taken in the ratio 4 : 1 : 0.16 : 3.1 mmol. In vitro drug release studies, microscopy, drug content, and related substance characterizations were done to formulate each strategy of spray drying prepared dry liposomes of isotretinoin. Results were compared with the USP monograph of isotretinoin. It was revealed that isotretinoin's liposomal formulation using ESD was having drug release according to the USP limits. Drug content was also according to the USP requirement; no free drug crystals were found in microscopy, multivesicular vesicles were found in shape, a particle size of up 60 µ was found. The ESD technique was a successful, time-effective, and cost-effective technique for preparing a liposomal drug delivery system for isotretinoin.

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“…The literature shows that most of the HPLC-CAD systems proposed for lipid compound analysis are based primarily on silica [ 3 , 12 , 13 , 14 ] or, as a second choice, on diol [ 4 , 15 , 16 ] columns although, C8, C18 and HILIC stationary phases were also used [ 17 , 18 , 19 ]. Mobile phases composed of solvent mixtures, such as n-heptane, n-hexane, isopropanol, chloroform, methanol, iso-octane or methyl t-butyl ether [ 3 , 4 , 6 , 12 , 15 ], were usually selected.…”

Section: Introductionmentioning

confidence: 99%

“…Mobile phases composed of solvent mixtures, such as n-heptane, n-hexane, isopropanol, chloroform, methanol, iso-octane or methyl t-butyl ether [ 3 , 4 , 6 , 12 , 15 ], were usually selected. Among the described studies, CAD detection was used mainly for testing pharmaceuticals (lipid excipients, liposomes) [ 18 , 20 ] or food samples such as edible oils, eggs and dairy products [ 4 , 12 , 13 , 15 , 16 ]. Despite the increasing use of CAD, a limited number of reports concern lipid studies in biological material such as cells or tissues [ 3 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of Glycerophospholipids in Biological Material Using High-Performance Liquid Chromatography with Charged Aerosol Detector HPLC-CAD—A New Approach for Isolation and Quantification

2022

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The method of using high-performance liquid chromatography with a charged aerosol detector method (HPLC-CAD) was developed for the separation and determination of phospholipids isolated from cell membranes. The established cell lines—normal and neoplastic prostate cells and normal skin fibroblasts and melanoma cells—were selected for the study. Chromatographic separation was performed in the diol stationary phase using a gradient elution based on a mixture of n-hexane, isopropanol and water with the addition of triethylamine and acetic acid as buffer additives. Taking the elements of the Folch and Bligh–Dyer methods, an improved procedure for lipid isolation from biological material was devised. Ultrasound-assisted extraction included three extraction steps and changed the composition of the extraction solvent, which led to higher recovery of the tested phospholipids. This method was validated by assessing the analytical range, precision, intermediate precision and accuracy. The analytical range was adjusted to the expected concentrations in cell extracts of various origins (from 40 µg/mL for PS up to 10 mg/mL for PC). Both precision and intermediate precision were at a similar level and ranged from 3.5% to 9.0%. The recovery for all determined phospholipids was found to be between 95% and 110%. The robustness of the method in terms of the use of equivalent columns was also confirmed. Due to the curvilinear response of CAD, the quantification was based on an internal standard method combined with a power function transformation of the normalized peak areas, allowing the linearization of the signal with an R2 greater than 0.996. The developed method was applied for the isolation and determination of glycerophospholipids from cell membranes, showing that the profile of the tested substances was characteristic of various types of cells. This method can be used to assess changes in metabolism between normal cells and neoplastic cells or cells with certain pathologies or genetic changes.

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Analytical profiling and stability evaluation of liposomal drug delivery systems: A rapid UHPLC-CAD-based approach for phospholipids in research and quality control

Cited by 20 publications

References 41 publications

Beyond Trial and Error: A Systematic Development of Liposomes Targeting Primary Macrophages

Beyond Trial and Error: A Systematic Development of Liposomes Targeting Primary Macrophages

Method Development and Characterization of Liposomal Formulation of Isotretinoin

Determination of Glycerophospholipids in Biological Material Using High-Performance Liquid Chromatography with Charged Aerosol Detector HPLC-CAD—A New Approach for Isolation and Quantification

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