Analytical Methods of Phenolic Compounds

Ignat, Ioana; Volf, Irina; Popa, Valentin I.

doi:10.1007/978-3-642-22144-6_56

Cited by 15 publications

(11 citation statements)

References 124 publications

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“…The extraction was favored when the stirring time was increased. The plant material, type of solvent used and extraction method are factors that may affect the yield of extraction of phenolic content [22]. Tables 3 and 4 present an analysis of variance (ANOVA) for the experimentally obtained data (y) and the values calculated from Equation 2 (SN L ).…”

Section: Phenolic Contentmentioning

confidence: 99%

Influence of Polar Solutions on the Extraction of Phenolic Compounds from Capulín Fruits (Prunus serotina)

et al. 2017

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<p>The phenolic compounds extraction of Prunus serotina Erhr with different ratios of acetone:water, methanol:water and ethanol:wa-ter was optimized using the Taguchi method. The factors evaluated were the solvent mixture and stirring time. The total phenolic content in extracts was assessed by the Folin-Ciocalteu method using gallic acid (GA) as the standard. The maximum value was 20.3 ± 0.86 mg <br />GAE g–1db using acetone:water (7:3, v/v) and 30 min of stirring time.</p>

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Section: Phenolic Contentmentioning

confidence: 99%

Influence of Polar Solutions on the Extraction of Phenolic Compounds from Capulín Fruits (Prunus serotina)

et al. 2017

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“…Various analytical techniques have been developed for the separation and detection of chlorogenic acids, where the most widely employed method is reversed phase high performance liquid chromatography (RP-HPLC), typically coupled with photodiode array (PDA) detection. [3,27] Another analytical method that has been used to confirm structural information about chlorogenic acids in a variety of food products, including apples, coffee beans and dried plums is HPLC coupled to mass spectrometry (MS). [28][29][30] MS is a superior platform for the analysis of phenolics due to its high sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

“…It allows for the identification and quantification of minor chlorogenic acids in a complex phenolic mixture. [27] To fully utilize MS, an understanding of analyte dissociation patterns during tandem (MS/MS) and multi-stage (MS n ) mass spectrometric analysis is required. The establishment of MS/MS fingerprints can be used for structural elucidations as well as for identifying unique product ions that can be used for targeted multiple reaction monitoring (MRM) quantitative analysis.…”

Section: Introductionmentioning

confidence: 99%

Analysis of a series of chlorogenic acid isomers using differential ion mobility and tandem mass spectrometry

Willems

Khamis

Mohammed-Saeid

et al. 2016

Analytica Chimica Acta

104

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Chlorogenic acids are among the most abundant phenolics found in the human diet. Of these, the mono-caffeoylquinic acids are the predominant phenolics found in fruits, such as apples and pears, and products derived from them. In this research, a comprehensive study of the electrospray ionization (ESI) tandem mass spectrometric (MS/MS) dissociation behavior of the three most common mono-caffeoylquinic acids, namely 5-O-caffeoylquinic acid (5-CQA), 3-O-caffeoylquinic acid (3-CQA) and 4-O-caffeoylquinic acid (4-CQA), were determined using both positive and negative ionization. All proposed structures of the observed product ions were confirmed with second-generation MS(3) experiments. Similarities and differences between the dissociation pathways in the positive and negative ion modes are discussed, confirming the proposed structures and the established MS/MS fingerprints. MS/MS dissociation was primarily driven via the cleavage of the ester bond linking the quinic acid moiety to the caffeic acid moiety within tested molecules. Despite being structural isomers with the same m/z values and dissociation behaviors, the MS/MS data in the negative ion mode was able to differentiate the three isomers based on ion intensity for the major product ions, observed at m/z 191, 179 and 173. This differentiation was consistent among various MS instruments. In addition, ESI coupled with high-field asymmetric waveform ion mobility spectrometry-mass spectrometry (ESI-FAIMS-MS) was employed for the separation of these compounds for the first time. By combining MS/MS data and differential ion mobility, a method for the separation and identification of mono-caffeoylquinic in apple/pear juice samples was developed with a run time of less than 1 min. It is envisaged that this methodology could be used to identify pure juices based on their chlorogenic acid profile (i.e., metabolomics), and could also be used to detect juice-to-juice adulteration (e.g., apple juice addition to pear juice).

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“…On the other hand, the plant material, type of solvent used and extraction method are factors that may affect the yield of extraction of phenolic content [21]. The extraction time can also affect the extraction performance due to the prolonged exposure of light and the stability of the phenolic compounds, however, in this research; the samples were protected from light during the entire extraction process and quantification.…”

Section: Discussionmentioning

confidence: 99%

Total Phenols, Flavonoids and Antioxidant Activity in Annona muricata and Annona purpurea Callus Culture

Ov¹,

o-Dom韓guez²,

Luj醤-Hidalgo³

et al. 2019

Phyton

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Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation was the treatment supplemented with 3 mg L-1 NAA for A. muricata (100%) while for A. purpurea in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mg L-1 the one that induced the greater formation of shoots for A. muricata (23 shoots/explant) and A. purpurea (28 shoots/explant). The content of total phenols, flavonoids and antioxidant activity was measured in the callus obtained from both species. The results showed that a higher content of total phenols was quantified in callus of A. purpurea (27.8 mg g-1 dw) compared to A. muricata (23.2 mg g-1 dw). The highest content of total flavonoids was observed in the callus of A. purpurea (8.0 μg g-1 dw). Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydracil radical assay. The concentration required for 50% inhibition (IC50) of the 2,2-diphenyl-1-picrylhydracil radicals were 4.22 μg mL-1 in methanolic extracts of callus of A. muricata, while in extracts of callus of A. purpurea was 2.86 μg mL-1 , in both cases was greater than that found for leaves. Callus culture of the species studied in this work represents an alternative for the production of natural antioxidants.

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Analytical Methods of Phenolic Compounds

Cited by 15 publications

References 124 publications

Influence of Polar Solutions on the Extraction of Phenolic Compounds from Capulín Fruits (Prunus serotina)

Influence of Polar Solutions on the Extraction of Phenolic Compounds from Capulín Fruits (Prunus serotina)

Analysis of a series of chlorogenic acid isomers using differential ion mobility and tandem mass spectrometry

Total Phenols, Flavonoids and Antioxidant Activity in Annona muricata and Annona purpurea Callus Culture

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